The invention relates to an intestine-regulating 
constipation-removing 
enzyme and a preparation method thereof. The 
enzyme comprises the following raw materials in percentage by weight: 5% of figs, 10% of 
sophora flowers, 15% of celery, 20% of pears, 20% of apples, 2% of radix paeoniae alba, 4% of plantain, 3% of liquorice, 20% of mulberries, 2% of 
dandelion, 20% of flaccid knotweed 
herb and 80% of coarse rice. The preparation method comprises the following steps: 1. 
steaming the coarse rice in the raw materials, alcoholizing the steamed coarse rice and the flaccid knotweed by 
hansenula anomala and 
saccharomyces cerevisiae, and after alcoholizing, adding water, acetifying by 
acetobacter pasteurianus and 
clostridium butyricum; 2. 
grinding the other materials, alcoholizing by honey yeasts, 
hansenula anomala, 
rhodotorula glutinis, 
saccharomyces cerevisiae and 
streptomyces microflavus, after finishing the steps 1 and 2, mixing according to a ratio of 1 to 1, lactifying by 
lactobacillus lactis and 
plant lactobacilli, and fermenting to obtain suspension liquid as the intestine-regulating 
constipation-removing 
enzyme. The intestine-regulating 
constipation-removing enzyme has the effects of promoting production of 
body fluid, moistening the 
lung and facilitating intestinal tract movement, and is suitable for 
crowds with constipation.