Microbial preparation for urban garbage percolate treatment and application thereof

A microbial agent and microbial technology, applied in the direction of microorganism-based methods, microorganisms, biological water/sewage treatment, etc., can solve the problems of difficult landfill leachate treatment, difficult backwashing, low power consumption, etc., and achieve improved removal effect , strong adaptability, and the effect of improving the removal rate

Inactive Publication Date: 2015-12-23
NINGBO BLUE ENVIRONMENT SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biological aerated filter integrates adsorption, oxidation and filtration, and has good treatment effect, less sludge, low power consumption, and good effluent quality. And other materials are used as filter materials, which have a large specificity, so the biggest problem encountered in operation is that it is difficult to backwash
Excessive ammonia nitrogen in traditional biological treatment will inhibit microorganisms, so it is currently difficult to treat landfill leachate to a discharge standard
Of course, the final check of RO technology has achieved the desired effluent quality, and the treatment cost is daunting

Method used

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  • Microbial preparation for urban garbage percolate treatment and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Preparation of liquid medium I: Take by weight 1 part of magnesium sulfate heptahydrate, 0.2 part of potassium chloride, 2.47 parts of sodium chloride, 0.3 part of sodium citrate, 1 part of yeast extract powder, 0.5 part of tryptone, add water to 100 parts , adjust the pH to be 7.0 and make liquid culture medium I;

[0018] Preparation of liquid medium II: weigh 1.2 parts of magnesium sulfate heptahydrate, 0.2 parts of potassium chloride, 2.4 parts of sodium chloride, 0.3 parts of sodium citrate, 0.2 parts of ammonium chloride, 0.5 parts of yeast extract powder, 0.15 parts of pancreatic Peptone, 0.7 part of starch, 0.5 part of glucose, add water to 100 parts, adjust pH to 7.0 to prepare liquid medium II.

Embodiment 2

[0019] Example 2 Preparation and application of microbial preparations for treating landfill leachate

[0020] (1) After activating the Erythrohalogenus bacterium Bacillus cereus, insert it into 1000 mL of the liquid culture medium I prepared in Example 1, and cultivate it at 28° C., 160 rpm, at normal pressure for 36 hours; insert the obtained bacterial liquid into 10 L of the liquid medium I prepared in Example 1 In the liquid culture medium I, at 28°C, 160rpm, normal pressure culture for 36h; then the obtained bacterial liquid was inserted into 200L liquid medium II prepared in Example 2 for large-scale production, and at 30°C, normal pressure culture for 48h to obtain Bacillus cereus suspension;

[0021] After activating the euryhalobacteria Bacillus marisflavi, insert it into 1000 mL of the liquid medium I prepared in Example 1, and cultivate it at 28° C., 160 rpm, at normal pressure for 36 h; insert the resulting bacterial liquid into 10 L of the liquid medium I prepared...

Embodiment 3

[0028] Example 3 Obtaining of transgenic euryhalohaline bacteria Bacillus cereus

[0029] Using DNAMAN software analysis, according to the amino acid sequence GenBank: WP_014597038.1 whole gene synthesis of the nucleotide sequence SL gene, the target fragment is obtained by amplification, and the target gene SL is obtained by PCR amplification (at the same time, the corresponding mutation is obtained by multiplex PCR Sites 59A / S, 82N / R, 100E / P, 123M / F, 139L / S, 147R / G, 179R / H, 191I / S, 203Q / K, 214A / R were introduced into the gene sequence, thus obtaining Different mutant genes), the PCR product was double-digested with restriction endonucleases, connected to the expression vector pSDK-1 that had also been double-digested, and the successfully verified recombinant plasmid was transferred from the large intestine by the usual method of plasmid transfer in the field. The bacilli are transformed into the euryhalobacteria bacillus cereus to obtain genetically engineered bacteria for ...

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Abstract

Provided is a microbial preparation for urban garbage percolate treatment and an application thereof. The invention relates to a microbial preparation for urban sewage treatment and an application thereof. Viable bacteria in the microbial preparation comprise euryhaline bacteria bacillus cereus, Bacillus marisflavi, rhodopuesdomonas pulstris and rhodopseudomonas acidophila, Nitrosomonas europaea, marine pseudomonas and paracoccus denitrificans and yeast rhodotorula glutinis. The invention further provides a production method of the microbial preparation. According to the microbial preparation, the effect on treating garbage percolate is obvious, operation is convenient, and the microbial preparation has better application and promotion value.

Description

technical field [0001] The invention belongs to the scope of microbial bacterial agents, and in particular relates to the preparation of a compound bacterial agent for efficient treatment of landfill leachate and its application in garbage treatment. Background technique [0002] With the improvement of human material living standards and the continuous increase of the number and scale of cities, municipal solid waste has become a serious social problem. According to relevant statistics, the average annual growth rate of garbage in my country has reached more than 10%, and it has become one of the countries with the most serious garbage siege in the world. handling and disposal. [0003] Since its inception, the sanitary landfill disposal method of garbage has been widely used in many countries due to its advantages of reliable technology, simple process, convenient management, low investment, low operating cost, wide application range, no strict requirements on garbage comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12N1/20C12N1/16C07K14/32C02F3/34C12R1/085C12R1/07C12R1/01C12R1/38C12R1/645
Inventor 冯国亮张正剑
Owner NINGBO BLUE ENVIRONMENT SCI & TECH CO LTD
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