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Tissue culturing, rapid propagating and transplanting method of Rhododendron mucronulatum Turcz.

A technology of tissue culture rapid propagation and rhododendron, applied in the biological field, can solve the problems of low rooting rate of tissue culture seedling transplantation that have not been reported before, and achieve the effects of high proliferation rate, stable proliferation, and high survival rate of transplanting

Inactive Publication Date: 2006-02-15
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The low rooting rate of tissue culture seedlings transplanted in the rapid propagation of Rhododendron has always been a concern
[0007] Tissue culture and rapid propagation of Rhododendron japonica has not been reported in domestic and foreign literature

Method used

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  • Tissue culturing, rapid propagating and transplanting method of Rhododendron mucronulatum Turcz.
  • Tissue culturing, rapid propagating and transplanting method of Rhododendron mucronulatum Turcz.

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Seeds of Rhododendron japonica collected in Yunmeng mountain area of ​​Beijing were soaked in sterile water for 30 to 60 minutes on a super-clean workbench, and the sunken seeds were removed, then sterilized in 0.1% HgCl2 solution for 5 minutes, and rinsed with sterile water. Rinse 3 times with sterile water, then sterilize with 0.1% HgCl2 for 5 minutes, rinse 4 times with sterile water, inoculate on sterile medium containing 3% sucrose and 0.7% agar. The culture conditions are 25±1°C, dark, after about 2 weeks of germination, transfer to the condition of 12 hours of light per day for cultivation, and about 2 months, take the epicotyl section of the shoot tip for primary culture.

[0031] The primary culture medium is rhododendron culture medium supplemented with 1mg / L IBA and 5mg / L ZT, the culture conditions are 25±1°C, 12 hours of light per day, and the light intensity is 2000-3000Lux. In about 60 days, a large number of clustered buds were induced, with about 30 bud ...

Embodiment 2

[0037] The culture procedures were all carried out according to Example 1, except that the source of the seeds was Yunmeng Mountain in Shandong, the explants of the primary culture were the shoot tips of sterile seedlings, and the cluster bud induction medium was improved. The B5 medium was supplemented with 6- Benzylaminoadenine 6-BA1mg / L, NAA0.1mg / L. The subculture medium is rhododendron medium supplemented with indole butyric acid IBA 1mg / L and 6-benzylaminoadenine 6-BA 5mg / L. The rooting induction medium is rhododendron medium supplemented with naphthalene acetic acid 2mg / L, 6-benzylaminoadenine 6-BA0.2mg / L.

Embodiment 3

[0039] The culture procedures were all carried out according to Example 1, except that the source of the seeds was Wuling Mountain, Hebei, and the primary culture medium was improved. The B5 medium was supplemented with 6-benzylaminoadenine 6-BA1.5mg / L, and Nai Acetate NAA0.2mg / L. The subculture medium is rhododendron medium supplemented with indole butyric acid IBA 1.5mg / L and 6-benzylaminoadenine 6-BA 10mg / L. The rooting induction medium is rhododendron medium supplemented with 1.5 mg / L of naphthalene acetic acid and 0.1 mg / L of 6-benzylaminoadenine 6-BA.

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Abstract

The invention disclose a technique for the tissue culturing and breeding of the red-welcome cuckoo as well as the technique for domestication and replanting, which comprises the first generation culture of inducing the clump sprout by applying the germ-free seedling as explant, subculture for enrichment-culture, root-growing inducing for the clump sprout, water-planting for domestication before replanting the tissue culture sprout, the final replanting to flowerpot and getting the red-welcome cuckoo. The invention is suitable for the red-welcome cuckoo from different places, the k-factor of the clump sprout is high, the root is uniform, and the survival rate after the water-culture domestication is high and is suitable for the red-welcome cuckoo commercial production.

Description

technical field [0001] The invention relates to tissue culture rapid propagation and domesticated transplanting technology of Rhododendron japonica. Specifically, it involves the complete production technology of taking the epicotyls of aseptic seedlings of Rhododendron japonica as explants, obtaining clustered buds through organogenesis, and multiplying, rooting, domesticating and transplanting in large quantities. Belongs to the field of biotechnology. Background technique: [0002] There are more than 900 species of Rhododendron (Rhododoendron) in the Rhododendron family (Ericaceae) in the world. They are concentrated in southeastern Asia, extending from the northwestern Himalayas to eastern Tibet, Myanmar and western China, and all the way to the south. Extending to Thailand and the Philippine Peninsula, 90% of Rhododendron species are distributed in this area. my country is the center of origin of Rhododendron, with 59% of the world's original species of Rhododendron....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00A01G7/00
Inventor 孙振元徐文忠赵梁军巨关升韩蕾杨学军
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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