Biological membrane used in vitro as well as preparation method and application of biological membrane

A biofilm and biological technology, applied in biochemical equipment and methods, microorganisms, animal cells, etc., can solve problems that need to be improved, improve tissue and cell adhesion function, accelerate cell growth, and prevent cell death or apoptosis Effect

Inactive Publication Date: 2019-03-01
武汉原生原代生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Therefore, current methods for using biofilms in vitro, in vitro tissue culture, and cell culture still need to be improved

Method used

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  • Biological membrane used in vitro as well as preparation method and application of biological membrane
  • Biological membrane used in vitro as well as preparation method and application of biological membrane
  • Biological membrane used in vitro as well as preparation method and application of biological membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 120

[0060] Example 1 Preparation of in vitro biofilm with 20% water content

[0061] (1) Take 100ml of double distilled water and pour it into a 200ml reaction vessel, place the reaction vessel on a mechanical stirrer and heat it to 55 degrees Celsius;

[0062] (2) Slowly add 5 parts by mass of collagen and silk protein (mass ratio 1:1), 10 parts by mass of gelatin and matrigel (mass ratio 1:1), 5 parts by mass of chitosan, hyaluronic acid And chondroitin sulfate (mass ratio 1:1:1), 1 mass part of alginate, 1 mass part of polyethylene glycol, and 1 mass part of cell matrix in the reaction vessel, mechanically stirred, stirring speed 150r / min, stirring time 10min;

[0063] (3) Slowly add vascular endothelial cell extracts of species with a mass percentage of 1% in sequence to the reaction vessel, and perform mechanical stirring at a stirring speed of 150 r / min for a stirring time of 10 min;

[0064] (4) Slowly add 1 mass part of polycarbonate and polycaprolactone (mass ratio 1:5...

Embodiment 2

[0069] The preparation of the in vitro biofilm of embodiment 2 60% water content

[0070] (1) Take 100ml of double distilled water and pour it into a 200ml reaction vessel, place the reaction vessel on a mechanical stirrer and heat it to 65 degrees Celsius;

[0071] (2) Slowly add 10 parts by mass of collagen and silk protein (mass ratio 1:1), 25 parts by mass of gelatin and Matrigel (mass ratio 1:1), 10 parts by mass of chitosan, hyaluronic acid And chondroitin sulfate (mass ratio 1:1:1), the alginate of 5 mass parts, the polyethylene glycol of 5 mass parts, the cell matrix of 5 mass parts are put in reaction container, carry out mechanical stirring, stirring speed 100r / min, stirring time 30min;

[0072] (3) Slowly add vascular endothelial cell extracts of 30% by mass species into the reaction vessel in turn, and perform mechanical stirring at a stirring speed of 100r / min for a stirring time of 30min;

[0073] (4) Slowly add 10 parts by mass of polycarbonate and polycaprol...

Embodiment 34

[0078] Example 3 Preparation of biofilms for in vitro use with 40% water content

[0079] (1) Take 100ml of double distilled water and pour it into a 200ml reaction vessel, place the reaction vessel on a mechanical stirrer and heat it to 60 degrees Celsius;

[0080] (2) Slowly add 8 parts by mass of collagen and silk protein (mass ratio 1:1), 20 parts by mass of gelatin and matrigel (mass ratio 1:1), 8 parts by mass of chitosan, hyaluronic acid and chondroitin sulfate (mass ratio 1:1:1), 3 mass parts of alginate, 4 mass parts of polyethylene glycol, and 2 mass parts of cell matrix in the reaction vessel, mechanically stirred, stirring speed 120r / min, stirring time 20min;

[0081] (3) Slowly add vascular endothelial cell extracts of 15% species in sequence to the reaction vessel, and perform mechanical stirring at a stirring speed of 120 r / min for a stirring time of 20 min;

[0082] (4) Slowly add 6 parts by mass of polycarbonate and polycaprolactone (mass ratio is 1:10) int...

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Abstract

The invention provides a biological membrane used in vitro. The biological membrane used in vitro comprises a matrix adsorber, a biological additive, a stent additive, a cross-linking agent and a permeation agent. The biological membrane used in vitro can keep the biological system of culture cells and stabilize growing status of the culture cells, and is suitable for detecting and analyzing drugscreening, drug therapeutic effect evaluation, drug adaptation disease evaluation, drug toxicity and side effect evaluation and drug compatibility study; meanwhile, the biological membrane used in vitro can detect and analyze biological activity and genetic characteristics of the tissue and the cells.

Description

technical field [0001] The present invention relates to the technical field of biofilms, in particular, the present invention relates to a biofilm for in vitro and its preparation method and application. Background technique [0002] Cell culture technology is to separate the tissue into individual cells, and culture them on culture vessels or square bottles under optimized artificial environmental conditions in vitro to make them grow and passage. Cells can also be frozen and revived, and then cultured again and grow. The tissue culture technology is to culture the tissue on a culture vessel or a square bottle under an optimized artificial environment in vitro, so that the cells in the tissue can grow and pass down, and the tissue can also be frozen and revived. However, the usual tissue and cell culture is on culture vessels or square bottles, which makes the cultured tissues and cells easy to lose their original biological characteristics, and genetics and other characte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12N5/071
CPCC12N5/0018C12N5/0602C12N5/067C12N5/0686C12N5/0688C12N5/0693C12N2533/30C12N2533/50C12N2533/54C12N2533/70C12N2533/72C12N2533/74C12N2533/80
Inventor 刘东旭
Owner 武汉原生原代生物医药科技有限公司
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