50 results about "In vitro tissue culture" patented technology

The invention discloses a method for endangered Chinese azalea in vitro tissue culture propagation and storage. The best rapid propagation system and storage system are built in the Chinese azalea tissue culture process. According to the method, an optimum explant selection method, an efficient multiple sterilization method, optimum culture medium components, adventive bud rooting, acclimatizating and transplanting condition control for Chinese azalea in vitro growth propagation and storage and other key technological problems are included, and finally large-quantity, rapid and high-quality propagation of Chinese azalea in a short time is achieved. The Chinese azalea test tube plantlet differentiation rate can reach 100%, the adventive bud growth coefficient month proliferation rate can reach 8-10.5, and the rooting rate and the acclimatizating survival rate are 85% or above after 40 d, so that the increasing requirement of people for Chinese azalea can be effectively met, then, damage by people to wild Chinese azalea is reduced, protection of the endangered wild Chinese azalea is facilitated, and huge scientific research value, economic value and ecologic value are achieved.

The invention provides a Terminalia mantaly Tricolor tissue culture and propagation method. The Terminalia mantaly Tricolor tissue culture and propagation method comprises the following steps of: carrying out tissue culture and rapid propagation under different hormone ingredients and concentration levels by using MS as basic culture medium, adding a natural organic mixture, and using the leaves or twigs of the Terminalia mantaly Tricolor as explants; carrying out disinfection treatment and inoculation induction to form a callus tissue; carrying out callus tissue differentiation, bud multiplication and rooting culture to form a complete plant seedling; transplanting the complete seedling in a raising substrate for growing into a normal Terminalia mantaly Tricolor plant, wherein the basic culture medium is based on MS culture media and supplemented with the ingredients including 6-benzylaminoadenine, 2,4-dichlorphenoxyacetic acid, naphthylacetic acid, sucrose, active carbon and the like. According to the in-vitro issue culture and rapid propagation method of Terminalia mantaly Tricolor disclosed by the invention, the tissue culture is applied to overcome the problems that the Terminalia mantaly Tricolor is long in seedling raising period and low in propagation coefficient, so that rapid industrialized seedling can be carried out for meeting the requirements of the market on the Terminalia mantaly Tricolor seedling.

The invention discloses a tissue culture method of raspberries. The method comprises the following steps: explant selection and disinfection, induction culture, enrichment culture, rooting culture, acclimatization and transplanting, and the like. The invention provides a raspberry in-vitro tissue culture rapid propagation packaged technology. Different hormones are utilized for adjustment in each phase to achieve the goals of enhancing the multiplication rate and reducing variation; the survived seedlings are convenient for transportation; and the method has the advantages of low requirements on the female parent material, low cost, short time, high survival rate and favorable product quality, and implements large-scale standardized production on raspberry seedlings.

The invention relates to a method of excysting and growing protozoal oocysts by in vitro tissue culture resulting in production of a continuous culture of merozoites. The invention also provides an economical and reliable supply of cultured Eimeria sp. for vaccine production, assays and research. Domesticated avians that have been vaccinated using the provied Eimeria sp. are also provided.

A porous chamber for tissue culture in vitro. The porous chamber includes a main body made of porous biologically absorbable polymer material, comprising an inner surface surrounding a hollow cavity, the hollow cavity having an aperture opened at an upper surface of the main body, wherein the aperture is communicated with outside of the main body for seeding tissue blocks in the hollow cavity, and an upper cover passing through the upper surface of the main body to seal the aperture to prevent the tissue blocks from outflowing.

The invention relates to a micro-needle based in vitro culture method for testicular tissue and applications thereof, and belongs to the technical field of biology. The method for in vitro culturing testicular tissue to promote proliferation of spermatogonial stem cells is provided. The method includes: soaking PEGDA micro-needles in a culture medium for replacement, so as to obtain a culture system including replaced PEGDA micro-needles and the culture medium; and adding isolated testicular tissue in the culture system to culture, so as to proliferate the spermatogonial stem cells in the isolated testicular tissue. The method improves situations of death of tissue centers during tissue culture in vitro, so that large tissue culture can be realized. Therefore, a physiological structure of the tissue can be maintained to the greatest extent, which is more in line with in vivo situations. In addition, the method can promote the proliferation of the spermatogonial stem cells in vitro, the self-renewal of the spermatogonial stem cells (SSCs) is supported, and the dryness of the spermatogonial stem cells can be maintained.

The invention relates to tissue culture media of Glossostigma elatinoides. The tissue culture media comprise an initial culture medium, a subculture medium and a rooting culture medium which are used for different stages. The initial culture medium prepared by adding potassium iodate, 2,4-D and paclobutrazol into an ER culture medium. The subculture medium is prepared by using an MS culture medium as the basis, regulating the concentration of ammonium nitrate to be 1200-1300mg / L and then adding urea, ABA, indole acetic acid, glutamine and activated carbon. The rooting culture medium is prepared by adding ammonium molybdate, TDZ and amylopectin into an N6 culture medium and using sodium hydroxide to regulate pH to be 7.0-7.2. When the in-vitro tissue culture media are used to perform tissue culture on the Glossostigma elatinoides, the Glossostigma elatinoides is high in survival rate, the obtained Glossostigma elatinoides seedlings are robust and has tenacious vitality, and the Glossostigma elatinoides can fast adapt to natural growth conditions after transplanting.

The invention discloses a method for in vitro tissue culture, reproduction and preservation of endangered sheep roe deer, establishes the best rapid propagation system and preservation system in the tissue culture process of sheep roe deer, including the most suitable selection method for explants, and is highly efficient. The multi-sterilization method, the most suitable medium composition for the in vitro growth and preservation of the sheep hemp , High-quality reproduction. The invention can make the differentiation rate of the test-tube seedlings of the goat deer reach up to 100%, the multiplication factor of the adventitious buds reaches 8-10.5 per month, and the rooting rate and domestication survival rate after 40 days are both over 85%. This can effectively meet people's increasing demand for sheep roe, thereby reducing people's damage to wild sheep roe, which is conducive to the protection of endangered wild sheep roe, and has great scientific research value, economic value and ecological value.

The invention discloses a method for creating germplasm resources of disease-resistant and high-quality head cabbage, which belongs to the technical field of cabbage breeding. This method selects the CMS male sterile line / maintainer line of cabbage with excellent comprehensive traits to be crossed with fertile cabbage plants with excellent comprehensive traits, and then adopts in vitro tissue culture from the hybrid offspring to recombine and screen for similar spherical shape, similar ripening period, and color. The sterile lines with similar comprehensive traits are backcrossed with similar fertile plants for 3 generations, and the fertile plants are self-crossed for one generation and then interplanted to breed new CMS male sterile lines with different ripeness, spherical shape and excellent color comprehensive traits / Maintainer lines, and other new excellent fertile strains. Through the improved culture of callus protoplasts, the frequency of protoplast regenerated plants can be increased, chimeras and polyploids can be eliminated, excellent fertile plants can be retained, and more and better new germplasm resources can be created.