Tissue culture media of Glossostigma elatinoides

A tissue culture medium, culture medium technology, applied in horticulture, botany equipment and methods, gardening tools/equipment and other directions, can solve the problems of quality and yield decline, planting benefit decline, virus accumulation, etc., to improve yield and quality, Improve the survival rate of carrier culture and the effect of strong vitality and tenaciousness

Active Publication Date: 2017-02-22
日照新睿招商发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional seedling breeding method of dwarf pearls has reduced yield, serious virus accumulation, species degradation, greatly reduced quality and yield, and reduced planting benefits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The tissue culture medium of dwarf pearls includes three kinds of culture medium at different stages, and the raw materials and component concentrations of each culture medium are as follows,

[0014] The primary medium is: on the basis of ER medium, add potassium iodate 0.1mg / L, add 2,4-D 0.6mg / L, add paclobutrazol 1.2mg / L;

[0015] The subculture medium is: on the basis of MS medium, first adjust the concentration of ammonium nitrate to 1200mg / L, then add urea 100mg / L, add ABA3mg / L, add indole acetic acid 0.5mg / L, add glutamine Amide 5.0mg / L, add activated carbon 200mg / L;

[0016] The rooting medium is: in N 6 On the basis of the culture medium, 0.01 mg / L ammonium molybdate, 1.5 mg / L TDZ, 130 mg / L amylopectin were added, and the pH was adjusted to 7.0-7.2 with sodium hydroxide.

Embodiment 2

[0018] The tissue culture medium of dwarf pearls includes three kinds of culture medium at different stages, and the raw materials and component concentrations of each culture medium are as follows,

[0019] The primary medium is: on the basis of ER medium, add potassium iodate 0.5mg / L, add 2,4-D 0.8mg / L, add paclobutrazol 1.3mg / L;

[0020] The subculture medium is: on the basis of MS medium, first adjust the concentration of ammonium nitrate to 1300mg / L, then add urea 120mg / L, add ABA 3.5mg / L, add indole acetic acid 0.8mg / L, add Glutamine 5.5mg / L, add activated carbon 300mg / L;

[0021] The rooting medium is: in N 6 On the basis of the culture medium, 0.02 mg / L ammonium molybdate, 2.0 mg / L TDZ, 150 mg / L amylopectin were added, and the pH was adjusted to 7.2 with sodium hydroxide.

Embodiment 3

[0023] The tissue culture medium of dwarf pearls includes three kinds of culture medium at different stages, and the raw materials and component concentrations of each culture medium are as follows,

[0024] The primary medium is: on the basis of ER medium, add potassium iodate 0.3mg / L, add 2,4-D 0.7mg / L, add paclobutrazol 1.25mg / L;

[0025] The subculture medium is: on the basis of MS medium, first adjust the concentration of ammonium nitrate to 1250mg / L, then add urea 110mg / L, add ABA 3.2mg / L, add indole acetic acid 0.7mg / L, add Glutamine 5.2mg / L, add activated carbon 250mg / L;

[0026] The rooting medium is: in N 6 On the basis of the culture medium, 0.015 mg / L of ammonium molybdate, 1.8 mg / L of TDZ and 140 mg / L of amylopectin were added, and the pH was adjusted to 7.1 with sodium hydroxide.

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PUM

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Abstract

The invention relates to tissue culture media of Glossostigma elatinoides. The tissue culture media comprise an initial culture medium, a subculture medium and a rooting culture medium which are used for different stages. The initial culture medium prepared by adding potassium iodate, 2,4-D and paclobutrazol into an ER culture medium. The subculture medium is prepared by using an MS culture medium as the basis, regulating the concentration of ammonium nitrate to be 1200-1300mg / L and then adding urea, ABA, indole acetic acid, glutamine and activated carbon. The rooting culture medium is prepared by adding ammonium molybdate, TDZ and amylopectin into an N6 culture medium and using sodium hydroxide to regulate pH to be 7.0-7.2. When the in-vitro tissue culture media are used to perform tissue culture on the Glossostigma elatinoides, the Glossostigma elatinoides is high in survival rate, the obtained Glossostigma elatinoides seedlings are robust and has tenacious vitality, and the Glossostigma elatinoides can fast adapt to natural growth conditions after transplanting.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to the tissue culture medium of dwarf pearls. Background technique [0002] Although dwarf pearls are aquatic plants with stems, the stems are not upright, but crawling and growing at the bottom. Because the creeping stolons will spread out in a diffuse shape, and finally cover the entire bottom, and the leaves on the stolons grow densely, which feels like a lawn. Generally, when planting dwarf pearls, in order to prevent the soft fine roots from being able to cling to the bottom sand, you should try to use finer sand in the choice of bottom sand. Dwarf pearls, barely 1 cm high, green creeping shoots with short root tissue, symmetrically grown spoon-shaped leaves 0.8 cm long and 0.3 cm wide, with blunt, slightly serrated tips, petioles almost as long as the leaves. Aerial leaf glands can produce short-stemmed white single-petal flowers, 0.3 cm in diameter, with 5 petals and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 不公告发明人
Owner 日照新睿招商发展有限公司
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