Quick reproduction of xiangxuelan
A technology of freesia, which is applied in the field of flower cultivation and reproduction, can solve the problems of low reproduction rate, inability to meet the needs of the market, and the inability of the freesia to meet the rapidly growing market demand, so as to achieve high reproduction coefficient, save land and shorten the breeding cycle. short effect
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[0020] The best embodiment of the present invention will be described in detail below by way of examples.
[0021] 1. Induction of callus or direct somatic embryos:
[0022] The explants were surface-sterilized with 70% alcohol under aseptic conditions, and then soaked in 0.1% mercury liter for 3-5 minutes. After being washed several times with sterile water, the explants were cut into small pieces of a certain length [young spike (1-2 mm), inflorescence rachis (2-3 mm), stem (1-3 mm), leaf (2 -3 mm), flower base (3-4 mm), mature embryo (1-1.5 mm), immature embryo (0.5-1 mm), root (1-2 mm)]. Small pieces of explants can be inoculated on the following two induction media to induce the formation of somatic embryos or callus.
[0023] Induction medium I (for direct induction of somatic embryos): N 6 +1~2mg / L 2,4-dichlorophenoxyacetic acid (2,4-D)+2~4mg / L 6-benzylaminopurine (6-BA) solid agar medium;
[0024] Induction medium II [for callus induction]: solid agar medium of MS+...
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