Quick reproduction of xiangxuelan

A technology of freesia, which is applied in the field of flower cultivation and reproduction, can solve the problems of low reproduction rate, inability to meet the needs of the market, and the inability of the freesia to meet the rapidly growing market demand, so as to achieve high reproduction coefficient, save land and shorten the breeding cycle. short effect

Inactive Publication Date: 2004-12-08
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are many disadvantages in the method of dividing into balls: 1. Low reproduction rate: unable to meet the needs o

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The best embodiment of the present invention will be described in detail below by way of examples.

[0021] 1. Induction of callus or direct somatic embryos:

[0022] The explants were surface-sterilized with 70% alcohol under aseptic conditions, and then soaked in 0.1% mercury liter for 3-5 minutes. After being washed several times with sterile water, the explants were cut into small pieces of a certain length [young spike (1-2 mm), inflorescence rachis (2-3 mm), stem (1-3 mm), leaf (2 -3 mm), flower base (3-4 mm), mature embryo (1-1.5 mm), immature embryo (0.5-1 mm), root (1-2 mm)]. Small pieces of explants can be inoculated on the following two induction media to induce the formation of somatic embryos or callus.

[0023] Induction medium I (for direct induction of somatic embryos): N 6 +1~2mg / L 2,4-dichlorophenoxyacetic acid (2,4-D)+2~4mg / L 6-benzylaminopurine (6-BA) solid agar medium;

[0024] Induction medium II [for callus induction]: solid agar medium of MS+...

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Abstract

A fast reproduction technology for the Freesia by in vitro tissue culture and soilless seedball reproduction includes such steps as using the young stachys, rhachis, stem, leaf, floral base, mature bud, root, etc of Freesia X hybrida as explant, preparing culture medium, inducing regeneration, and special treating. A freeze storage method for the calli is also disclosed. Its advantages are high reproduction coefficient, short reproduction period, and no need of soil.

Description

technical field [0001] The invention belongs to flower cultivation and propagation technology, in particular to in vitro tissue culture and soilless bulb propagation technology of modern polyploid Freesia x hybrida. Background technique: [0002] Freesia is also known as freesia, calamus, fragrant iris, and tuberose. The classification of freesia is a perennial bulbous herbaceous flower belonging to the genus of the family Iridaceae. [0003] The modern polyploid Snowberry is cultivated from the wild diploid Snowberry by artificial breeding technology, and its somatic cell chromosome number is 33 or 44. Modern polyploid Snowland is a rare cut flower variety, which has the advantages of long flowering period, tall plant, large flower, bright color, fragrant flower and long flower arrangement period. Another feature of Fragrant Snowland is that its flowering period is in winter and spring, and it can be supplied to the market in early spring. It can be cultivated as a cut f...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王丽
Owner NORTHEAST NORMAL UNIVERSITY
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