Ilex crenata golden gem tissue culture breeding method

A technology of tortoiseshell holly and golden leaf, applied in the field of tissue culture and propagation of golden leaf tortoiseshell holly, can solve the problems of low reproduction coefficient and survival rate, limited popularization and application, and slow reproduction speed, so as to improve survival rate, shorten the reproduction cycle, and accelerate reproduction speed effect

Active Publication Date: 2015-01-07
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conventional propagation methods of golden leaf tortoiseshell holly include cutting propagation and grafting propagation. The propagation cycle of cutting propagation is about 1 year, the survival rate is about 90%, and the reproduction coefficient is less than or equal to 1; the

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0029] Example 1

[0030] Select the young stems with axillary buds of the golden leaf tortoiseshell wintergreen as the explants, and rinse the explants repeatedly for 1 hour under running water, add an appropriate amount of detergent to soak and clean them for 3 times, soak and shake to rinse off Then move to the ultra-clean workbench for the next step of disinfection. The disinfection treatment is as follows: first soak in 75% alcohol by volume concentration for 20s, rinse the shaking explants 3 times with sterile water for 7 minutes each time; then transfer to 0.1% mercury (HgCl) 2 ) Sterilize for 8 minutes, rinse with sterile water repeatedly, and absorb the moisture on the surface of the explant with sterile paper.

[0031] Inoculate the sterilized golden leaf tortoiseshell wintergreen explants on the primary culture medium. The formula of the primary culture medium is: MS+ZT 0.5mg / L+NAA0.1mg / L+ sucrose 25mg / L+ agar 7mg / L, pH value 5.6 . The culture conditions are: a sterile...

Example Embodiment

[0038] Example 2

[0039] Select the young stems with axillary buds of the golden leaf tortoiseshell wintergreen as the explants, and rinse the explants repeatedly for 1 hour under running water, add an appropriate amount of detergent to soak and clean them for 3 times, soak and shake to rinse off Then move to the ultra-clean workbench for the next step of disinfection. The disinfection treatment is as follows: first soak in 75% alcohol by volume for 20s, rinse the oscillating explants with sterile water 5 times for 6 minutes each time; then transfer to 0.1% mercury (HgCl) 2 ) Sterilize for 9 minutes, rinse with sterile water repeatedly, and absorb the water on the surface of the explant with sterile paper.

[0040] Inoculate the sterilized golden leaf tortoiseshell holly explants on the primary culture medium. The formula of the primary culture medium is: MS+ZT1.0mg / L+NAA0.1mg / L+sucrose 25mg / L+agar 7mg / L, PH value 5.8 . The culture conditions are: a sterile culture room with a t...

Example Embodiment

[0047] Example 3

[0048] Select the young stems with axillary buds of the golden leaf tortoiseshell wintergreen as the explants, and rinse the explants repeatedly for 1 hour under running water, add an appropriate amount of detergent to soak and clean them for 3 times, soak and shake to rinse off Then move to the ultra-clean workbench for the next step of disinfection. The disinfection treatment is as follows: first soak in 75% alcohol by volume for 20s, rinse the shaking explants with sterile water 5 times, each rinse for 5 minutes; then transfer to 0.1% mercury (HgCl) 2 ) Sterilize for 8 minutes, rinse with sterile water repeatedly, and absorb the moisture on the surface of the explant with sterile paper.

[0049] Inoculate the sterilized golden leaf tortoiseshell holly explants on the primary culture medium. The formulation of the primary culture medium is: MS+ZT 0.8mg / L+NAA0.1mg / L+sucrose 25mg / L+agar 7mg / L, PH value 5.7 . The culture conditions are: a sterile culture room wi...

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PUM

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Abstract

The invention discloses an ilex crenata golden gem tissue culture breeding method which comprises steps of sterilization, primary culture, secondary culture, strong seedling culture, rooting culture, seedling exercising and transplanting. Through the ilex crenata golden gem tissue culture breeding method disclosed by the invention, the breeding coefficient of ilex crenata golden gem can be improved, the breeding speed of the ilex crenata golden gem can be speeded up, the survival rate of the ilex crenata golden gem can be increased, technical support is provided for rapid breeding of excellent ilex crenata golden gem seedlings, and the ever-increasing demand of the market on the ilex crenata golden gem can be satisfied.

Description

technical field [0001] The present invention relates to the field of holly propagation, in particular to a tissue culture propagation method of golden leaf tortoise shell holly. Background technique [0002] The golden-leaf tortoiseshell holly is a colorful-leaved plant of the holly genus in the Ilex family. It has the characteristics of evergreen, low temperature resistance, and low and compact plants. It is a new variety of colorful leaves introduced from abroad in my country in recent years. The old leaves are dark green and shiny, and the new leaves are golden yellow, with extremely bright color. In recent years, tortoiseshell holly has been in great demand in the greening industry at home and abroad, so its propagation speed should be accelerated to meet its increasing market demand. The conventional propagation methods of golden leaf tortoiseshell holly include cutting propagation and grafting propagation. The propagation cycle of cutting propagation is about 1 year, ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 郑凯丁久玲王海珍李大功蔡泓宇
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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