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Ilex crenata golden gem tissue culture breeding method

A technology of tortoiseshell holly and golden leaf, applied in the field of tissue culture and propagation of golden leaf tortoiseshell holly, can solve the problems of low reproduction coefficient and survival rate, limited popularization and application, and slow reproduction speed, so as to improve survival rate, shorten the reproduction cycle, and accelerate reproduction speed effect

Active Publication Date: 2015-01-07
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conventional propagation methods of golden leaf tortoiseshell holly include cutting propagation and grafting propagation. The propagation cycle of cutting propagation is about 1 year, the survival rate is about 90%, and the reproduction coefficient is less than or equal to 1; the propagation cycle of grafting propagation is more than one year, and the survival rate is 60%. %-80%, the reproduction coefficient is less than or equal to 1, due to its slow reproduction speed, low reproduction coefficient and survival rate limit its popularization and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Select the young stems with axillary buds of the golden-leaf tortoiseshell holly as explants, place the explants under running water and rinse them repeatedly for 1 hour, add an appropriate amount of detergent to soak and wash them for 3 times, shake them while soaking, and rinse them clean Then move to the ultra-clean workbench for the next step of disinfection. Disinfection treatment is as follows: soak the explants in 75% alcohol for 20 seconds, rinse and shake the explants with sterile water for 3 times, each time for 7 minutes; then transfer to 0.1% mercury chloride (HgCl 2 ) for 8 minutes, rinsed repeatedly with sterile water, and then blotted the moisture on the surface of the explants with sterile paper.

[0031] Inoculate the sterilized explants of the holly-leaved tortoise shell on the primary medium, the formula of the primary medium is: MS+ZT 0.5mg / L+NAA0.1mg / L+sucrose 25mg / L+agar 7mg / L, pH value 5.6 . The culture conditions are: a sterile culture room wit...

Embodiment 2

[0039] Select the young stems with axillary buds of the golden-leaf tortoiseshell holly as explants, place the explants under running water and rinse them repeatedly for 1 hour, add an appropriate amount of detergent to soak and wash them for 3 times, shake them while soaking, and rinse them clean Then move to the ultra-clean workbench for the next step of disinfection. Disinfection treatment is as follows: soak the explants in 75% alcohol for 20 seconds, rinse and vibrate the explants 5 times with sterile water for 6 minutes each time; then transfer to mercuric chloride (HgCl 2 ) for 9 minutes, rinsed repeatedly with sterile water, and then blotted the moisture on the surface of the explants with sterile paper.

[0040] Inoculate the sterilized Ilex aureus explants on the primary culture medium, the formula of the primary culture medium is: MS+ZT1.0mg / L+NAA0.1mg / L+sucrose 25mg / L+agar 7mg / L, pH value 5.8 . The culture conditions are: a sterile culture room with a temperature...

Embodiment 3

[0048] Select the young stems with axillary buds of the golden-leaf tortoiseshell holly as explants, place the explants under running water and rinse them repeatedly for 1 hour, add an appropriate amount of detergent to soak and wash them for 3 times, shake them while soaking, and rinse them clean Then move to the ultra-clean workbench for the next step of disinfection. Disinfection treatment is as follows: soak the explants in 75% alcohol for 20 seconds, rinse and vibrate the explants 5 times with sterile water, and rinse for 5 minutes each time; then transfer to mercury chloride (HgCl 2 ) for 8 minutes, rinsed repeatedly with sterile water, and then blotted the moisture on the surface of the explants with sterile paper.

[0049] Inoculate the sterilized Ilex aureus explants on the primary culture medium, the formula of the primary culture medium is: MS+ZT 0.8mg / L+NAA0.1mg / L+sucrose 25mg / L+agar 7mg / L, pH value 5.7 . The culture conditions are: a sterile culture room with a ...

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PUM

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Abstract

The invention discloses an ilex crenata golden gem tissue culture breeding method which comprises steps of sterilization, primary culture, secondary culture, strong seedling culture, rooting culture, seedling exercising and transplanting. Through the ilex crenata golden gem tissue culture breeding method disclosed by the invention, the breeding coefficient of ilex crenata golden gem can be improved, the breeding speed of the ilex crenata golden gem can be speeded up, the survival rate of the ilex crenata golden gem can be increased, technical support is provided for rapid breeding of excellent ilex crenata golden gem seedlings, and the ever-increasing demand of the market on the ilex crenata golden gem can be satisfied.

Description

technical field [0001] The present invention relates to the field of holly propagation, in particular to a tissue culture propagation method of golden leaf tortoise shell holly. Background technique [0002] The golden-leaf tortoiseshell holly is a colorful-leaved plant of the holly genus in the Ilex family. It has the characteristics of evergreen, low temperature resistance, and low and compact plants. It is a new variety of colorful leaves introduced from abroad in my country in recent years. The old leaves are dark green and shiny, and the new leaves are golden yellow, with extremely bright color. In recent years, tortoiseshell holly has been in great demand in the greening industry at home and abroad, so its propagation speed should be accelerated to meet its increasing market demand. The conventional propagation methods of golden leaf tortoiseshell holly include cutting propagation and grafting propagation. The propagation cycle of cutting propagation is about 1 year, ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 郑凯丁久玲王海珍李大功蔡泓宇
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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