In-vitro tissue culture and rapid propagation method of Terminalia mantaly Tricolor

A technology of tissue culture and Terminalia jinye, which is applied in the field of in vitro tissue culture and rapid propagation of Terminalia jinye, can solve the problems of weather conditions, time-consuming and labor-consuming, and inability to produce seedlings on a large scale, and achieve easy operation and synchronization Good, fast reproduction effect

Inactive Publication Date: 2013-10-09
武爱龙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after many years of observation, no flowering and fruiting of this tree species have been found, so the sexual reproduction of this tree species is limited. At present, only asexual reproduction is carried out. There are three ways of asexual reproduction, one is cutting, one is grafting, and the other is It is in vitro tissue culture and rapid propagation. Through investigation, it is found that the seedlings of Terminalia jinye are generally produced by grafting. The method is primitive and time-consuming and labor-intensive. At the same time, it is affected by weather conditions and cannot produce seedlings on a large scale.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The Tissue Culture Propagation Method of Terminalia Jinye comprises the following steps:

[0030] A, selection and disinfection of explants: After selecting the leaves on the mother plant of Terminalia Jinye with good growth, wash them with tap water for 10 minutes, and put the leaves on the aseptic ultra-clean bench under aseptic conditions. Rinse with 75% alcohol for a while (within one second), transfer to 0.1% mercuric solution for disinfection for 5 minutes, rinse with sterile water for 3 times (note that the leaves should not be soaked in alcohol), cut off the leaves for later use.

[0031] B. Induction of explants: inoculate the whole leaves spared in step A on the callus induction medium, the induction medium is MS+6-BA 3 mg / L+2.4-D 0.1 mg / L+ NAA 0.2 mg / L, and adding natural organic mixture, the culture time is 45 days, and the light green callus is formed at the incision of the leaf.

[0032] C, induction and differentiation of adventitious buds: the callus ob...

Embodiment 2

[0037] The Tissue Culture Propagation Method of Terminalia Jinye comprises the following steps:

[0038] A, selection and disinfection of explants: After selecting the tender shoots on the mother plant of Terminalia Jinye with good growth and strong growth, wash them with tap water for 10 minutes, and cut them into 3 cm long tender shoots on a sterile ultra-clean bench. Branches, under aseptic conditions, soak the tender branches in 75% alcohol for 1 minute, transfer to 0.2% mercuric chloride solution for disinfection for 10 minutes, rinse 4 times with sterile water, cut off the sterilized and browned ends Finally, shorten it to 2 cm long and set aside.

[0039] B, explant induction: inoculate the twigs 2.0 cm long in step A on the callus induction medium, the callus induction medium is MS+6-BA 4mg / L+2.4-D 0.5 mg / L L+NAA 0.4mg / L, and adding natural organic mixture, the culture time is 50 days, and the light green callus is formed at the incision of the twig.

[0040] C. Indu...

Embodiment 3

[0045] The Tissue Culture Propagation Method of Terminalia Jinye comprises the following steps:

[0046] A, selection and disinfection of explants: After selecting the leaves on the mother plant of Terminalia Jinye with good growth, wash them with tap water for 5 minutes, and put the leaves on the aseptic ultra-clean table under aseptic conditions. Rinse with 75% alcohol for a while (within one second), transfer to 0.2% mercuric solution for disinfection for 5 minutes, rinse with sterile water 4 times (note that the leaves should not be soaked in alcohol), cut off the leaves for later use.

[0047] B. Induction of explants: inoculate the whole leaves that have been spared in step A on the callus induction medium, the induction medium is MS+6-BA 2 mg / L+2.4-D 0.2 mg / L+ NAA 0.1 mg / L, and natural organic mixture was added, and the culture time was 40 days, and a light green callus was formed at the incision of the leaf.

[0048] C, induction and differentiation of adventitious bu...

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PUM

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Abstract

The invention provides a Terminalia mantaly Tricolor tissue culture and propagation method. The Terminalia mantaly Tricolor tissue culture and propagation method comprises the following steps of: carrying out tissue culture and rapid propagation under different hormone ingredients and concentration levels by using MS as basic culture medium, adding a natural organic mixture, and using the leaves or twigs of the Terminalia mantaly Tricolor as explants; carrying out disinfection treatment and inoculation induction to form a callus tissue; carrying out callus tissue differentiation, bud multiplication and rooting culture to form a complete plant seedling; transplanting the complete seedling in a raising substrate for growing into a normal Terminalia mantaly Tricolor plant, wherein the basic culture medium is based on MS culture media and supplemented with the ingredients including 6-benzylaminoadenine, 2,4-dichlorphenoxyacetic acid, naphthylacetic acid, sucrose, active carbon and the like. According to the in-vitro issue culture and rapid propagation method of Terminalia mantaly Tricolor disclosed by the invention, the tissue culture is applied to overcome the problems that the Terminalia mantaly Tricolor is long in seedling raising period and low in propagation coefficient, so that rapid industrialized seedling can be carried out for meeting the requirements of the market on the Terminalia mantaly Tricolor seedling.

Description

technical field [0001] The invention relates to a method for in vitro tissue culture and rapid propagation of Terminalia jinye. Background technique [0002] Terminalia jinye is a cultivated variety of Terminalia microphylla, the plant height can reach 10m, and the side branches grow in whorls and spread out horizontally. The main trunk is straight, the tree is beautiful, and the layers are distinct. The greening and beautifying effects are very good, and it is suitable for garden greening. In recent years, the side branches are distributed horizontally in layers, and the light green leaves have milky white or milky yellow spots, which are very popular in greening projects in South China. Five or six years ago, all the plants used in the market came from Taiwan, so the price was relatively expensive. Each plant with a DBH of 5 to 6 cm cost 3,000 yuan. Around 2000, some nurseries in South China began to use Terminalia lobata with a height of 1 meter as rootstock for graftin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 武爱龙
Owner 武爱龙
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