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Tissue culture method of raspberries

A tissue culture and raspberry technology, which is applied in the field of raspberry tissue culture, can solve the problems of easy shedding, high requirements for parent materials, small fruits, etc., and achieves the effects of improving proliferation rate, good product quality and reducing variation.

Inactive Publication Date: 2017-10-13
广西桂平市蒙圩镇柱强种养专业合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conventional propagation methods of raspberry include root division method, branch method, cutting method and layering method, etc., but the propagation speed of the above-mentioned propagation methods is slow, and the seedlings produced have poor reproductive ability, small fruit, easy to fall off, low yield, and are harmful to the mother. This material has high requirements, a large amount of consumption, and is also limited by seasons. It is difficult to carry out large-scale, standardized production and large-scale promotion of fine varieties.
In addition, the current method of using tissue culture to reproduce and produce seedlings has high cost and long culture period.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for tissue culture of raspberry, including the following steps:

[0025] (1) Explant selection and disinfection: select the 2.5cm stem tip or the budded stem segment of the raspberry seedling as the raspberry explant, after removing the leaves, rinse with running water for 25 minutes; if the raspberry explant has fluff on the surface After washing with soap or Tween, rinse with running water for 30 minutes, and then perform disinfection treatment; the disinfection of the explants is: in a sterile ultra-clean table, the washed explants are sequentially placed at a volume concentration of 75% Sterilize in alcohol for 25s, sterilize in 0.1% mercuric chloride by mass concentration for 7 minutes, sterilize in sodium hypochlorite solution with volume concentration of 1.5% for 20 minutes, and then rinse with sterile water 5 times;

[0026] (2) Induction culture: The sterilized raspberry explants are inoculated into the induction medium under aseptic conditions, and culture...

Embodiment 2

[0031] A method for tissue culture of raspberry, including the following steps:

[0032] (1) Explant selection and disinfection: select the 3cm stem tip or budded stem segment of the raspberry seedling as the raspberry explant, after removing the leaves, rinse with running water for 35 minutes; if the surface of the raspberry explant has fluff, Wash with soap or Tween, then rinse with running water for 40 minutes, and then perform disinfection; the disinfection of the explants is: in a sterile ultra-clean table, the washed explants are sequentially placed in a volume concentration of 75% Sterilize in alcohol for 35s, sterilize in mercuric chloride with a mass concentration of 0.15% for 9 minutes, sterilize in a sodium hypochlorite solution with a volume concentration of 2% for 25 minutes, and rinse with sterile water for 6 times.

[0033] (2) Induction culture: the sterilized raspberry explants are inoculated into the induction medium under aseptic conditions, and cultured at 26°C ...

Embodiment 3

[0038] A method for tissue culture of raspberry, including the following steps:

[0039] (1) Explant selection and disinfection: select the 2.6~2.9cm stem tip or budded stem segment of the raspberry seedling as the raspberry explant, after removing the leaves, rinse with running water for 28~32min; if the raspberry explant There are fluff on the surface, wash with soap or Tween, rinse with running water for 33~37min, and then perform disinfection treatment; the disinfection of the explants is: in a sterile ultra-clean table, the washed explants are sequentially Sterilize in 75% alcohol by volume for 27~33s, sterilize in 0.11~0.14% mercury chloride by mass concentration for 7.5~8.5min, and sterilize in 1.6~1.9% by volume sodium hypochlorite solution 21~24min, then rinse with sterile water 5~6 times, that’s all;

[0040] (2) Induction culture: the sterilized raspberry explants are inoculated into the induction medium under aseptic conditions, and cultured at 23~25℃ for 23~27 days, w...

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PUM

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Abstract

The invention discloses a tissue culture method of raspberries. The method comprises the following steps: explant selection and disinfection, induction culture, enrichment culture, rooting culture, acclimatization and transplanting, and the like. The invention provides a raspberry in-vitro tissue culture rapid propagation packaged technology. Different hormones are utilized for adjustment in each phase to achieve the goals of enhancing the multiplication rate and reducing variation; the survived seedlings are convenient for transportation; and the method has the advantages of low requirements on the female parent material, low cost, short time, high survival rate and favorable product quality, and implements large-scale standardized production on raspberry seedlings.

Description

Technical field [0001] The invention belongs to the technical field of plant cultivation, and specifically relates to a method for tissue culture of raspberries. Background technique [0002] Raspberry, also known as raspberry, belongs to the genus Rubus in the Rosaceae family. It is a small perennial shrub deciduous fruit tree. Raspberry has wide adaptability and strong growth. Widely distributed in northern my country, raspberry is the third-generation golden fruit that has the fastest development in the world and integrates nutrition and health care. At present, the research and development of raspberry mainly focus on the introduction of raspberry and the processing and utilization of fruit. The conventional propagation methods of raspberry include root division method, ramification method, cutting method and layering method, etc. However, the above-mentioned propagation method has a slow propagation speed, and the cultivated seedlings have poor breeding ability, small frui...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 岑新标
Owner 广西桂平市蒙圩镇柱强种养专业合作社
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