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High-compliance tissue engineering blood vessel preparation template and tissue engineering blood vessel

A tissue engineering and compliance technology, which is applied in the direction of blood vessels, tissue regeneration, and human tubular structure devices, can solve the problems of stimulating thrombus formation, intimal hyperplasia, and prone to slipping, etc., to reduce the incidence of intimal hyperplasia, enhance Anti-kink performance, effect of reducing fiber exposure

Active Publication Date: 2020-09-29
领博生物科技(杭州)有限公司
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  • Abstract
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Problems solved by technology

However, the above-mentioned previous studies also reflected the following problems: First, some skeleton fibers in the tissue-engineered vascular lumen prepared after subcutaneous implantation using the above-mentioned templates were exposed, which would stimulate thrombus formation; The outer surface of the prepared tissue-engineered blood vessel is relatively smooth, and it is easy to slip after implantation, which reduces the integration rate of the blood vessel and the implantation site; third, the compliance of the blood vessel prepared by the above method is not good; fourth, the in vivo implantation intimal hyperplasia
[0005] Studies have found that one of the main reasons for intimal hyperplasia after implantation of artificial blood vessels is the mismatch between the compliance of artificial blood vessels and the compliance of natural blood vessels, which in turn causes the mechanical stimulation of the regenerated smooth muscle cells on the artificial blood vessels to be different from that of the natural blood vessels. The mechanical stimulation received does not match, causing excessive proliferation of smooth muscle cells, resulting in intimal hyperplasia

Method used

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  • High-compliance tissue engineering blood vessel preparation template and tissue engineering blood vessel
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  • High-compliance tissue engineering blood vessel preparation template and tissue engineering blood vessel

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preparation example Construction

[0050] The invention also discloses a method for preparing a skeleton body, which includes the following steps:

[0051] Step 1. Prepare a support layer using conventional support preparation techniques;

[0052] Step 2. Finally, a degradable layer is prepared and set on the outside of the support layer using conventional scaffold preparation technology;

[0053] In step 3, the support layer is prepared again outside the degradable layer using conventional support preparation techniques;

[0054] Step 4. Repeat steps 2-3 until the required number of layers is reached.

[0055] The invention also discloses a high-compliance tissue engineering blood vessel, which is prepared by using the high-compliance tissue engineering blood vessel preparation template as a template.

[0056] The highly compliant tissue engineering blood vessel includes an acellular matrix layer and supporting fibers; the supporting fibers are the fibers in the template supporting layer, and the acellular m...

Embodiment 1

[0074] The high-compliance tissue engineering blood vessel preparation template in this embodiment includes a skeleton body and an inner core; the inner core is arranged inside the skeleton body, and the skeleton body includes a support layer and a degradation layer; the degradation layer is arranged on Between two adjacent support layers; the degradation layer is 3 layers.

[0075] The template preparation process includes the following steps:

[0076] 1. A silicone tube with an outer diameter of 2 mm and an inner diameter of 1 mm is used as the inner core, and the outer diameter of the inner core determines the inner diameter of the prepared highly compliant tissue engineered blood vessel.

[0077] 2. Using polycaprolactone (PCL) as raw material, the first support layer is formed on the surface of the inner core by melt spinning technology. The fiber diameter in the first support layer is 20 μm, and the crossing angle between fibers in the first support layer is is 30°, and...

Embodiment 2

[0089] The high-compliance tissue engineering blood vessel preparation template in this embodiment includes a skeleton body and an inner core; the inner core is arranged inside the skeleton body, and the skeleton body includes a support layer and a degradation layer; the degradation layer is arranged on Between two adjacent support layers; the degradation layer is two layers.

[0090] The template preparation process includes the following steps:

[0091] 1. A polyurethane tube with an outer diameter of 4 mm and an inner diameter of 2 mm is used as the inner core, and the outer diameter of the inner core determines the inner diameter of the prepared tissue engineering blood vessel.

[0092] 2. Using poly(lactide-caprolactone) copolymer (PLCL) as a raw material, the first support layer (2) is formed on the surface of the inner core by melt spinning technology, and the fiber diameter of the first support layer is 40 μm. The crossing angle between the fibers of the first support...

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Abstract

The invention belongs to the field of tissue engineering, and particularly relates to a high-compliance tissue engineering blood vessel preparation template. The high-compliance tissue engineering blood vessel preparation template comprises a framework main body (1) and an inner core (2), wherein the inner core (2) is arranged inside the framework main body (1); the outer diameter of the inner core (2) is matched with the inner diameter of the framework main body (1); the main body of the framework main body (1) comprises support layers (11) and degradation layers (12) in alternate arrangement; the inner surface and the outer surface of the framework main body are the support layers (11); and the degradation layers (12) are made of degradable materials. The high-compliance tissue engineering blood vessel preparation template is used for being implanted under the skin of an animal or being used for in-vitro tissue culture; then, the inner core (2) is pulled away; and after the decellularization treatment, the high-compliance tissue engineering blood vessel can be prepared. The high-compliance tissue engineering blood vessel preparation template has the beneficial effects that the tissue engineering blood vessel prepared by using the high-compliance tissue engineering blood vessel preparation template has good compliance; the intimal hyperplasia can be inhibited.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and in particular relates to a highly compliant tissue engineering blood vessel preparation template and tissue engineering blood vessels. Background technique [0002] Vascular disease is the disease with the highest fatality rate in the world. Stenosis or blockage of blood vessels leads to reduced blood flow and lack of nutrients, resulting in tissue or organ damage, which is the main cause of the disease. Usually manifested as cerebrovascular disease, coronary heart disease, peripheral arterial disease. According to the prediction of the World Health Organization, by 2030, the number of people dying of cardiovascular-related diseases in the world will increase to 23.3 million each year. Vascular transplantation is still a routine method for the treatment of such diseases. At present, the collection and use of patients' autologous blood vessels (such as great saphenous vein, internal thoracic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/36A61L27/58A61F2/06A61L27/50A61L27/18A61L27/24
CPCA61L27/507A61L27/50A61L27/58A61L27/3604A61F2/06A61L27/18A61L27/24A61L2430/40
Inventor 王恺张杰许鹏赟
Owner 领博生物科技(杭州)有限公司
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