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Method for rapid seedling of red-core hylocereus undatus through in vitro tissue culture

A kind of red pitaya, in vitro technology, applied in the field of plant reproduction, can solve the problems of poor effect, lack of in vitro fast propagation system of red pitaya, and achieve the effects of shortening production cycle, obvious synergistic effect, and robust growth.

Active Publication Date: 2018-11-09
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Above-mentioned method is the tissue culture method of common dragon fruit, and these methods are ineffective in the tissue culture application of red heart dragon fruit
[0004] At present, there is still a lack of a set of stable and efficient in vitro rapid propagation system for red dragon fruit

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the method for the rapid growth of seedlings of the red pitaya tissue culture disclosed in this embodiment comprises the steps:

[0028] (1) Selection and disinfection of explants: Take the red heart pitaya stem section with a thorn seat, soak the explant with a detergent solution and brush the thorn seat part with a brush, and then rinse with water for 30 minutes; then remove the explant Soak in 75% alcohol for 10 seconds, take it out and rinse it twice with sterile water, then put the explants in 0.1% mercuric solution for detoxification for 5-8 minutes, take it out and rinse it repeatedly with sterile water for 6-8 times, Pull out the thorns with a sterilized camera;

[0029] (2) Induction culture: The sterilized and punctured explants were planted vertically in MS + 6-BA 2.0mg / L + NAA 1.5mg / L + sucrose 20g / L + agar 6.0g / L, the pH value was On the induction medium of 5.8~6.2, and the thorn seat of the explant was immersed in the culture, placed in the ...

Embodiment 2

[0034]Embodiment 2: the method for the rapid growth of seedlings of the red pitaya tissue culture disclosed in this embodiment comprises the following steps:

[0035] (1) Selection and disinfection of explants: Take the red heart pitaya stem section with a thorn seat, soak the explant with a detergent solution and brush the thorn seat part with a brush, and then rinse with water for 30 minutes; then remove the explant Soak in 75% alcohol for 10 seconds, take it out and rinse it twice with sterile water, then put the explants in 0.1% mercuric solution for detoxification for 5-8 minutes, take it out and rinse it repeatedly with sterile water for 6-8 times, Pull out the thorns with a sterilized camera;

[0036] (2) Induction culture: The sterilized and punctured explants were planted vertically in MS + 6-BA 3.0mg / L + NAA 1.0mg / L + sucrose 30g / L + agar 4.0g / L, the pH value was On the induction medium of 5.8~6.2, and the thorn seat of the explant was immersed in the culture, place...

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Abstract

The invention belongs to the technical field of plant propagation and particularly relates to a method for rapid seedling of red-core hylocereus undatus through in vitro tissue culture. The method comprises the following steps: by taking stems with areole of the red-core hylocereus undatus as an explant, disinfecting, then picking off small thorns, respectively carrying out induction culture, enrichment culture, rooting culture and transplanting culture. The improved areole is immersed into a culture medium, the sprouting of gemmule can be obviously accelerated, and the quantity of the femmuleis increased; in the rooting culture process of the gemmule, through primary sprouting of adventitious roots, namely transplanting culture, the production period of seedlings of the red-core hylocereus undatus is greatly shortened, the growth coefficient is 3-5 times, the rooting percentage is 100%, and the rate of survival after transplanting is 96% or above, the production period of the seedlings is about 90d. the method is a new technology for rapid propagation of the red-core hylocereus undatus, is suitable for industrialized, large-scale and intensive production of the seedlings of the red-core hylocereus undatus.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a method for quickly growing seedlings of red pitaya by tissue culture in vitro. Background technique [0002] Red pitaya, also known as red pitaya, is a dragon fruit ( Hylocereus undatus ) is a new type of fruit with good health benefits. Its pulp contains a large amount of natural anthocyanins and is red. The fruit of red dragon fruit is rich in nutrition, rich in calcium, phosphorus, iron and other minerals and vitamins, and also rich in plant albumin, anthocyanin and high water-soluble dietary fiber, and its sweetness is much higher than ordinary Dragon fruit has changed the common understanding of "dragon fruit is good-looking or not delicious" in the past, and is deeply loved by people. It has been widely introduced and cultivated in tropical and subtropical regions of my country. The demand for seedlings is large, and the supply of seedlings is in sho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 何承忠田斌李旦罗一然丁文沙韩国伟何小帆
Owner SOUTHWEST FORESTRY UNIVERSITY
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