A kind of Melaleuca prothallus induction and propagation method

A technology for prothallus and Melaleuca tower, which is applied in the field of prothallus induction and proliferation of Melaleuca tower, can solve the problems of browning, explant contamination, inability to grow, differentiate and proliferate, etc., so as to avoid sterilization difficulties and overcome the effect of not growing

Inactive Publication Date: 2011-12-21
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These explants have problems such as difficulty in explant sterilization, low rate of sterile explant acquisition,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Selection of explants

[0026] Gather Melaleuca from the wild [ Huperzia serrata (Thunb.) Trev.] sporangia, rinsed with tap water;

[0027] (2) Surface sterilization of explants

[0028] Dip the cleaned sporangia in an ethanol solution with a mass fraction of 70% for 30-60s in an ultra-clean workbench; 2 ) solution for 5-8 minutes, and then rinsed with sterile water for 3-5 times, as explants induced by Melaleuca prothallus;

[0029] (3) Induction culture of prothallus

[0030] The explants are inoculated on the prothallus induction medium, the composition of the prothallus induction medium is: 0.2 mg / L-2 mg / L of 3-indolebutyric acid is added to the conventional MS medium. Cultivate for 10-12 weeks at a culture temperature of 22±2°C, a light intensity of 300 Lx, and a light time of 12 / d-16 / d, and grow green prothallus tissue from the explants. The induction frequency is 70%;

[0031] (4) Proliferation of the prothallus

[0032] Cut the prothallus tissue obta...

Embodiment 2

[0034] (1) Selection of explants

[0035] Gather Melaleuca from the wild [ Huperzia serrata (Thunb.) Trev.] sporangia, rinsed with tap water;

[0036] (2) Surface sterilization of explants

[0037] Dip the cleaned sporangia in an ethanol solution with a mass fraction of 70% for 30-60s in an ultra-clean workbench; 2 ) solution for 5-8 minutes, and then rinsed with sterile water for 3-5 times, as explants induced by Melaleuca prothallus;

[0038] (3) Induction culture of prothallus

[0039] The explants were inoculated on the prothallus induction medium (the composition of the prothallus induction medium was the same as in Example 1), the culture temperature was 22±2°C, the light intensity was 1500 Lx, and the light time was 12 / d~16 / Cultivate under the condition of d for 10-12 weeks, grow green prothallus tissue from the explant, and the induction frequency of prothallus is 15%;

[0040] (4) Proliferation of the prothallus

[0041] Cut the prothallus tissue obtained in s...

Embodiment 3

[0043] (1) Selection of explants

[0044] Gather Melaleuca from the wild [ Huperzia serrata (Thunb.) Trev.] sporangia, rinsed with tap water;

[0045] (2) Surface sterilization of explants

[0046] Dip the cleaned sporangia in an ethanol solution with a mass fraction of 70% for 30-60s in an ultra-clean workbench; 2 ) solution for 5-8 minutes, and then rinsed with sterile water for 3-5 times.

[0047] Squeeze the sterilized sporangia to release the spores from the sporangia, and then collect the spores by centrifugation as explants induced by the prothallus of Melaleuca;

[0048] (3) Induction culture of prothallus

[0049] Take the spores in step (2), first dilute the spores to 100 / ml suspension with conventional MS liquid medium, inoculate 1ml of the spore suspension evenly on the prothallus induction medium (prothallium induction medium The composition is the same as that in Example 1), cultured for 10-12 weeks under the condition that the culture temperature is 22±2°C...

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PUM

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Abstract

The invention discloses a method for inducing and multiplying a prothallium of Huperzia serrata. The method comprises the following steps of taking a sporangium or a spore as an explant, performing in vitro tissue culturing on the explant; establishing an induced system of the prothallium of the Huperzia serrata, wherein the induction frequency of the prothallium reaches 15 percent; and under an artificial regulation, performing multiplication culturing for the prothallium until the month multiplication time reaches 5 times. The method is easy, has high efficiency, and can be used for establishing an import technical base for artificial propagation for the Huperzia serrata.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for inducing and proliferating a melaleuca prothallus. Background technique [0002] Melaleuca is Huperziaceae (Huperziaceae) Huperzia ( Huperzia Bernh .) Plant Hupermus serrata ( Huperzia serrata (Thunb.) Trevis), synonym: lice grass, dwarf fir tree, lice medicine. Melaleuca pagoda was first recorded in "Plant Names and Facts", and its nature and flavor are pungent, bitter, and flat. It has the effects of dissipating blood stasis, reducing swelling, detoxifying and relieving pain. In 1972, Chinese scientific and technological workers reported that huperzine A isolated from this plant has the effect of relaxing striated muscles. Afterwards, a large number of studies found that huperzine A is a highly efficient, low toxicity, reversible and highly selective inhibitor of acetylcholinesterase. agent. Due to the significant medical effects and ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 郭斌尉亚辉包日双徐玲玲
Owner NORTHWEST UNIV
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