30results about How to "Increase product volume" patented technology

According to the invention, based on Illumina common tag library sequencing and methylation normal sequencing and combining a common library tag sequencing method, a new method for accurate detection of whole genome methylation sites by utilizing trace genome DNA (deoxyribonucleic acid) is built. According to the method, in the construction of a methylation library by using trace genome DNA, exogenous DNA is innovatively added to carry out bisulfite high-efficiency co-treatment; and at the same time, fragment size selection is not needed, and PCR (polymerase chain reaction) amplification is directly carried out after bisulfite treatment. By the method, the defects that in the common methylation sequencing, samples can not be mixed, the PCR amplification efficiency is low and the trace DNAsample can not be researched are overcome.

The invention discloses an air conditioner room for cultivating edible fungi. The air conditioner room comprises a cultivating room, and an air conditioner device communicated with the internal part of the cultivating room is arranged outside the cultivating room; the air conditioner device comprises a case, and the internal chamber of the case is divided in upper, medium and lower three chambers; the upper chamber is communicated with the medium chamber; an air feeder is arranged in the upper chamber, the air feeder is communicated with a blast pipe in the cultivating room; the medium chamber is communicated with the cultivating chamber through a return air pipe; the lower chamber is separated into an exhaust chamber and an internal chamber by a vertical partition board and a full-heat exchanger; an exhaust fan is arranged in the exhaust chamber; the internal chamber is separated into a return air chamber and an intake chamber by a horizontal partition board; an intake electric valve is arranged on an air inlet of the intake chamber; the air return chamber is communicated with the return air pipe through an exhaust pipe; the exhaust pipe is provided with an exhaust electric valve; the intake chamber is communicated with the medium chamber through the full-heat exchanger; and the exhaust chamber is communicated with the return air chamber through the full-heat exchanger. The room provided by the invention can realize ventilation, adjust temperature and humidity and air flow speed and realize sterilization, so that the growth cycle of the edible fungi is steady, and both the yield and quality of the edible fungi can be increased.

Provided herein are methods of increasing the efficiency of biomass saccharification. In particular, the methods include ways of avoiding feedback inhibition during the production of useful products.

The invention provides a production method of a dihydromyrcenol. The technological processes are shown as the figure and carried out through the following step of: introducing dihydromyrcenol (hereafter referred to as DHM), water and a low-viscosity organic solvent X via a DHM feed pipe 1 and a water and solvent input pipe2 into a forced circulation radiation flow fixed bed (hereafter referred to as IRRFB) reactor 3. The pressure in the reactor is 0.12-0.28MPa; the IRRFB reactor 3 is provided with a barrel-shaped housing; a centre pipe 5 with two sealed ends and apertures covered on a side wall is arranged in the housing; and a catalyst fixed bed layer 4 made of metal nets and filled with catalysts is arranged outside the centre pipe 5. A reaction solution is pumped into the centre pipe 5, is introduced into the catalyst fixed bed layer 4 at a high speed in a radiation manner via the apertures on the side wall, penetrates through the catalyst fixed bed layer 4 to enter the housing of the reactor 3 and carries out a next round of circulation via a first pipeline 6 at the bottom. Firstly, oil and water are separated in a reacted liquid and a product is rectified twice, so that a product with a purity of 99.61% is obtained; and the once conversion rate is 10.6%, and the selectivity is 98.1%.

The invention belongs to the technical field of food processing, and provides a gluten-free grain fermentation type flour product. The gluten-free grain fermentation type flour product is prepared from the following raw materials in parts by weight: 1.0 to 1.2 parts of gluten-free grain flour, 0.1 to 0.2 part of plant protein powder without gluten proteins, 0.01 to 0.03 part of yeast powder, 0.001to 0.05 part of TG enzyme, 0.01 to 0.05 part of lactobacillus plantarum powder, 0.01 to 0.03 part of salt, 0.01 to 0.02 part of bread improver and 0.6 to 0.8 part of water. According to the invention, after the raw materials are matched in the ratio, gas tightness of a gluten-free grain dough can be improved, and elasticity and a product volume of the gluten-free grain fermentation type steamed flour product in an initial stage of steaming are improved, so that the finally obtained gluten-free grain fermentation type steamed flour product is rich in nutrition, good in taste and excellent in flavor.

The invention provides a metal and alloy hollow powder preparing method. The method comprises the steps that a proper amount of main salt, sodium hypophosphite and sodium hydroxide are dissolved in deionized water to obtain a 50-200ml solution, and the solution concentration of the main salt, the sodium hypophosphite and the sodium hydroxide is 0.2-1.0mol / L, 0.3-3.0mol / L and 0.5-3.0mol / L respectively; a mechanical stirring rod is placed in the middle mouth of a three-mouth flask, the mail salt solution is poured into the three-mouth flask through an edge port, the three-mouth flask is placed in an oil bath which is 80-150 DEG C to be preheated, and the other two solutions are placed in the same oil bath to be preheated for 3-5min at the same time; the sodium hydroxide solution is poured into the three-mouth flask slowly through the edge port and then stirred for 5-10min with the mechanical stirring rod at a speed of 100-1000rpm; a proper number of stabilizers are added to prevent colloid decomposition; the sodium hypophosphite solution is poured into the three-mouth flask through the edge port and then stirred for 1-8h with the mechanical stirring rod at a speed of 100-500rpm till reaction ends.

The invention discloses an alpha-glycosidase gene mutant and application thereof in preparation of 2-O-alpha-D-glucosyl-L-ascorbic acid, and the alpha-glycosidase gene mutant is prepared by mutating tyrosine at 270 into phenylalanine, or / and mutating tryptophan at 373 into leucine, or / and mutating methionine at 411 into phenylalanine, or / and mutating tryptophan at 273 into leucine, or / and mutating methionine at 411 into leucine. Or / and mutating arginine at the 491st site into lysine, or / and mutating tryptophan at the 504th site into leucine. The mutant is simple to prepare, and the yield of AA-2G generated through catalysis is improved. Under the same conditions, the specific activity of the mutant is obviously improved compared with that of the original enzyme, the highest yield of AA-2G generated through catalysis can reach 18.9 g / L, and the conversion rate of AA-2G is obviously improved compared with that of the original enzyme.

The invention discloses a high-yield and energy-saving electrolytic furnace for rare-earth metals. The high-yield and energy-saving electrolytic furnace comprises a cathode, a graphite anode, electrolyte, a graphite groove, a crucible, an inner steel sleeve, an outer steel sleeve, a filler layer, diamantane and a furnace panel. The high-yield and energy-saving electrolytic furnace has the characteristics that the furnace building cost is low, the service life is long, the heat balance is good, the content of impurities in a product is less, the yield is high, the energy is saved and the operation is convenient and the like.

The embodiment of the invention provides a liquid material evaporation numerical simulation method in a spray drying tower and a storage medium, and belongs to the field of liquid material evaporation. Through the above technical scheme, the full-size three-dimensional geometric model of the spray drying tower is established according to the geometric parameters of the spray drying tower and meshgeneration is carried out; solving is carried out, an output variable result is obtained for analyzing the evaporation process of the liquid material; compared with the prior art that measurement cannot be directly carried out in a drying tower, a data result obtained by adopting experience of a designer and a method of carrying out small-scale trial amplification on an experimental result of a small device has deviation from an actual result, through establishing and solving a full-size three-dimensional geometric model of the spray drying tower, the obtained result is more precise.

The invention relates to a multifunctional module combined ocean platform and a use method. The multifunctional module combined ocean platform comprises a functional deck module (1), lower supporting auxiliary single modules (2) and connecting modules (3), wherein the functional deck module (1) floats on the water surface, and the connecting modules (3) are used for connecting the functional deck module (1) with the auxiliary single modules (2). The functional deck module (1) is of an unpowered flat plate structure and used for configuring operation equipment, each auxiliary single module (2) is of a cylindrical structure with a circular or polygonal horizontal section, a generating set is arranged in each auxiliary single module (2) and used for driving a jet propulsion device by the aid of an electrically-driven pump, and according to different use requirements, the ocean platform can be variously combined to use so as to achieve different requirements. The multifunctional module combined ocean platform is capable of arriving at a destination by means of self-propulsion, maneuvering performance can be improved, deck operation area can be widened, material storage capacity can be improved, power configuration can be enhanced, and operation capability is improved. The multifunctional module combined ocean platform adopts the modular generalized design, so that product batches are effectively increased, assembly line production of marine work equipment is realized, cycle is shortened, and cost is reduced.

The invention relates to a permanent magnetic ferrite sintering electric kiln and belongs to the field of kilns. The invention solves a technical problem of providing a permanent magnetic ferrite sintering electric kiln capable of cleaning products falling onto a heating element in time. The permanent magnetic ferrite sintering electric kiln of the invention comprises a kiln body, wherein the kiln body comprises a kiln wall and a furnace chamber enclosed by the kiln wall; and a sundries discharge channel is arranged below the furnace chamber. The permanent magnetic ferrite sintering electric kiln of the invention can clean the products falling onto the heating element in time, avoid the scrapping of the heating element and the products falling onto the heating element, improve the productquality at each time of sintering, reduce product sintering cost, improve product qualification rate and temperature control flexibility, improve the thermal insulation effect of the furnace chamber and reduce production cost.

The invention relates to a flexible positioning wear-less vibrating sand filling device for fuse protectors. The flexible positioning wear-less vibrating sand filling device comprises a vertical variable-frequency vibrator, a support, a prepressed track type sand filling valve component, a flexible compression positioning component, a flexible sand filling nozzle component, a sand filling cylinder, a sand filling sensor component, a multi-station turn plate and a vibrator power switch. The vertical variable-frequency vibrator is arranged on the multi-station turn plate, the support is arranged on the vertical variable-frequency vibrator, the prepressed track type sand filling valve component, the flexible compression positioning component and the flexible sand filling nozzle component are arranged on a cylinder movable seat of the sand filling cylinder, the cylinder movable seat is arranged on the support, the sand filling sensor component is arranged on the vertical variable-frequency vibrator and corresponds to the flexible sand filling nozzle component, and fuse protectors are arranged on a fuse protector support on the vertical variable-frequency vibrator. Compared with the prior art, the flexible positioning wear-less vibrating sand filling device for the fuse protectors has the advantages of small wear to workpieces, rapidness, convenience, high efficiency and the like.

The invention relates to an automatic screw tightening device which comprises a rack part, a guide part, a moving tightening part, a screw guiding and cutter relieving part and an auxiliary part, wherein the rack part consists of a bottom plate, a round guide rail and an upper cover board; the guide part consists of a moving guide plate, a screw guide barrel and a guide cylinder; the moving tightening part consists of a screwdriver rod, a torque transmission nail, an adjusting spring, a connecting sleeve, a moving plate, a moving cylinder and a pneumatic motor; the screw guiding and cutter relieving part consists of a spring, a lifting pin, a shaft pin, a swing iron and a fixing part; and the auxiliary part consists of a die and a control part. In the working process, a workpiece is fed into the die, a screw is conveyed to the screw guide barrel through a pneumatic conveying device and the screw is guided and limited to be separated from the screw guide barrel through the screw guiding and cutter relieving part. When the screw is tightened, the pneumatic motor of the moving tightening part is inflated to drive the screwdriver rod to rotate through a connecting sleeve and the torque transmission nail, and the moving cylinder is inflated to drive the moving tightening part to move so as to screw the screw tightly. Compared with the prior art, the automatic screw tightening device has the advantages of high efficiency, high quality, wide application range and the like.

The invention belongs to the technical field of fermentation engineering, and particularly relates to a high-temperature fermentation regulation and control method of erythritol. The method comprises the following steps of: (1) activating and culturing strains into a domesticated single plant; (2) inoculating the domesticated single plant in the step (1) into a first-stage seed culture medium, and carrying out seed amplification culture to a logarithmic phase to obtain a first-stage seed solution; (3) inoculating the first-stage seed solution obtained in the step (2) into a second-stage seed culture medium according to a proportion of 0.01-0.1% (V / V), and carrying out amplification culture to the logarithmic phase to obtain a second-stage seed solution; and (4) transferring the second-stage seed solution obtained in the step (3) into a sterilized fermentation culture medium according to the proportion of 10% (V / V,), and carrying out aerobic fermentation to obtain the erythritol. According to the method, temperature adaptive domestication culture needs to be carried out on strains from strain domestication, seed amplification culture and seed fermentation, human intervention is carried out on a thallus growth environment, the temperature in a fermentation process is increased in a stepped manner, thalli are effectively stimulated, the product amount is increased, the yield of the erythritol is increased, and the production cost is reduced.

The invention discloses a double-layer injection mold for a PVC trunking switch box cover, which comprises a front mold assembly and a rear mold assembly matched with the front mold assembly. Guide mechanisms penetrate the front mold assembly and the rear mold assembly, a gating system communicating with the front mold assembly and the rear mold assembly is arranged on the front mold assembly, andthe rear mold assembly is connected with an ejecting mechanism; and three parting surfaces are arranged between the front mold assembly and the rear mold assembly, wherein a first parting surface anda third parting surface are respectively provided with first in-mold cutting mechanisms and second in-mold cutting mechanisms for shearing a main runner and a gate in the gating system. By adopting amold structure with the three parting surfaces, products are ranked and superimposed into upper and lower layers, and by adopting an in-mold cutting technology in which the main runner and the gate can be cut at the same time, the main runner is not stuck in a mold after breaking off; and after the parting surfaces are opened, both the products and runners can smoothly fall off. Without increasing the length and width of the mold, the product quantity can be doubled, thereby reducing energy consumption and improving production efficiency.

The invention discloses a pipeline catalytic reaction device for a liquid phase. The pipeline catalytic reaction device for the liquid phase comprises a reaction pipeline, wherein the reaction pipeline comprises a deceleration branch pipe and a reaction branch pipe, and a plurality of segments of ceramic ball joints are arranged in the deceleration branch pipe; a plurality of partition plates arearranged in parallel in the reaction branch pipe, and the partition plate is provided with an overflow plate and meshes. The ceramic ball joints of which the ceramic ball diameters are gradually increased are arranged in the deceleration branch pipe, and the flow rate of a liquid phase reactant is reduced by a physical means; the plurality of partition plates are arranged in parallel in the reaction branch pipe, and the contact time between the liquid phase reactant and a catalyst is effectively prolonged due to the blocking of the overflow plates; the liquid phase reactant reacts once while passing through one partition plate, so that the reactant fully reacts, the amount of the product is increased, and the transport and the reaction are integrated into one body, thereby shortening the total reaction time. The catalytic reaction device has a simple structure, is easy to manufacture, has low economic cost, and good practicability, and is suitable for widespread promotion.

A load assembly includes a supply mechanism structured to supply a plurality of container closures, and a conveyance assembly comprising a star wheel including a number of load pockets structured to receive the container closures and move the container closures from the supply mechanism to a processing assembly. The star wheel has an outer perimeter. Each of the load pockets is structured to receive a corresponding one of the container closures inboard of the outer perimeter of the star wheel.

The invention discloses an intrinsic safety and explosion isolation separated pneumatic valve positioner. According to the intrinsic safety and explosion isolation separated pneumatic valve positioner, an amplifier assembly is arranged in an intrinsic cavity; a circuit board, a nozzle assembly, a sensor assembly and other parts are arranged in an explosion isolation cavity. The size and weight ofthe explosion isolation positioner are greatly reduced, the mounting reliability of the positioner is enhanced, and accordingly, the explosion isolation positioner can be mounted on a valve conveniently.

The invention discloses soybean protein isolate gluten-free rice bread and a preparation method thereof, and belongs to the technical field of grain processing. The problems that existing gluten-free rice bread is difficult to form, prone to aging, lack of nutrition and the like are solved. The gluten-free rice bread uses rice flour as a main material and soybean protein isolate as a modifier, and comprises buckwheat flour, eggs, white granulated sugar, butter, yeast, water and other raw materials. The mixed flour of the rice flour and the soybean protein isolate is subjected to high-pressure treatment and then forms the dough together with the buckwheat flour, the gluten-free rice bread with good quality is obtained in cooperation with a bread making process, and the defects that the gluten-free rice bread is high in quality, nutritional, prone to aging and the like are overcome.

The invention discloses PCR premix liquid of a fluorescent quantitative PCR thermal cycler and a preparation method and application of the PCR premix liquid and belongs to the technical field of biology. According to the PCR premix liquid, DNA double chains are embedded into SYTO13, namely a fluorescence indicator, and dye CXR serves as reference fluorescence. The PCR premix liquid is specially developed for the fluorescent quantitative PCR thermal cycler, and the application effect of the premix liquid in the fluorescent quantitative PCR instrument is stable. SYTO13 serves as the fluorescence indicator, the dye has no inhibition for a PCR reaction, fluorescence intensity is higher, and higher specificity is achieved when the dye is combined with the double chains. The reference fluorescence dye CXR with proper concentration is added, and the PCR premix liquid can be matched with the fluorescent quantitative PCR instrument. Warm start Taq DNA polymerase is selected and used, the PCR experimental period is shortened while a primer is prevented from reacting with a template at normal temperature, and the PCR reaction efficiency and specificity are improved.

The invention provides a production method of a dihydromyrcenol. The technological processes are shown as the figure and carried out through the following step of: introducing dihydromyrcenol (hereafter referred to as DHM), water and a low-viscosity organic solvent X via a DHM feed pipe 1 and a water and solvent input pipe2 into a forced circulation radiation flow fixed bed (hereafter referred to as IRRFB) reactor 3. The pressure in the reactor is 0.12-0.28MPa; the IRRFB reactor 3 is provided with a barrel-shaped housing; a centre pipe 5 with two sealed ends and apertures covered on a side wall is arranged in the housing; and a catalyst fixed bed layer 4 made of metal nets and filled with catalysts is arranged outside the centre pipe 5. A reaction solution is pumped into the centre pipe 5, is introduced into the catalyst fixed bed layer 4 at a high speed in a radiation manner via the apertures on the side wall, penetrates through the catalyst fixed bed layer 4 to enter the housing of the reactor 3 and carries out a next round of circulation via a first pipeline 6 at the bottom. Firstly, oil and water are separated in a reacted liquid and a product is rectified twice, so that a product with a purity of 99.61% is obtained; and the once conversion rate is 10.6%, and the selectivity is 98.1%.

The invention provides a metal and alloy hollow powder preparing method. The method comprises the steps that a proper amount of main salt, sodium hypophosphite and sodium hydroxide are dissolved in deionized water to obtain a 50-200ml solution, and the solution concentration of the main salt, the sodium hypophosphite and the sodium hydroxide is 0.2-1.0mol / L, 0.3-3.0mol / L and 0.5-3.0mol / L respectively; a mechanical stirring rod is placed in the middle mouth of a three-mouth flask, the mail salt solution is poured into the three-mouth flask through an edge port, the three-mouth flask is placed in an oil bath which is 80-150 DEG C to be preheated, and the other two solutions are placed in the same oil bath to be preheated for 3-5min at the same time; the sodium hydroxide solution is poured into the three-mouth flask slowly through the edge port and then stirred for 5-10min with the mechanical stirring rod at a speed of 100-1000rpm; a proper number of stabilizers are added to prevent colloid decomposition; the sodium hypophosphite solution is poured into the three-mouth flask through the edge port and then stirred for 1-8h with the mechanical stirring rod at a speed of 100-500rpm till reaction ends.

The invention discloses a biosynthesis method of quercetin glycosides. The gene of the glycosyltransferase UGT73G1 mutant is connected with the sucrose synthase gene to obtain a recombinant plasmid, and a recombinant bacterium containing a double-enzyme system is constructed; the recombinant bacterium is inoculated in LB In liquid culture medium, cultivate, add inducer lactose to induce, fermented liquid is centrifuged to collect bacteria, the bacteria are broken, centrifuged to collect supernatant to obtain crude enzyme liquid; dissolve quercetin or isoquercetin in DMSO, add crude Enzyme solution, sucrose, reaction, and centrifugation to obtain supernatant quercetin-3,4'-diglucoside. The amino acid sequence of the glycosyltransferase UGT73G1 mutant is an amino acid sequence in which V371A and / or F381Y mutations occur in the sequence shown in SEQ ID NO:1. The mutant is simple to prepare, has a large yield, and realizes an increase in the yield of quercetin-3,4' diglucoside.

A textile mill system and associated method include a plurality of spinning mills each having textile machines. A computer system determines adapted machine parameters for the textile machines and processes within the spinning mills. The computer system includes a receiving and transmitting section configured to receive operational information from the spinning mills and the textile machines, and a first database configured to store the received operational information. A processing section includes an optimizer section with a neural network, wherein the neural network uses the operational information stored in the first database with processes for or derived from supervised or unsupervised machine or deep learning to determine the adapted machine parameters.

The invention provides a cloning method of microRNA precursor genes of moso bamboos. The cloning method comprises the following steps: respectively optimizing a PCR amplification system and PCR reaction conditions of the microRNA precursor genes of the moso bamboos, reducing the dosage of primers, increasing the number of formworks, controlling an annealing temperature to be 66-68 DEG C, controlling the number of times of cycle to be 30-32, changing the gel concentration, the voltage and the electrophoresis time of agarose gel electrophoresis, and realizing the purpose of efficiently cloning the microRNA precursor genes of the moso bamboos. The method disclosed by the invention is high in specificity, the quantity of target gene products is high, a base mispairing phenomenon is avoided in a key mature sequence region of the precursor genes, and the homology of the obtained precursor gene sequence and a target gene sequence is as high as 100%; the further construction of a plant expression vector is facilitated, the cloning method is used for cultivating genetically modified plants, and the cloning method has favorable application prospects in the respects of heredity improvement and molecular cultivation of farming and forestry crops.