The invention belongs to the technical field of fermentation engineering, and particularly relates to a high-temperature fermentation regulation and control method of erythritol. The method comprises the following steps of: (1) activating and culturing strains into a domesticated single plant; (2) inoculating the domesticated single plant in the step (1) into a first-stage seed culture medium, and carrying out seed amplification culture to a logarithmic phase to obtain a first-stage seed solution; (3) inoculating the first-stage seed solution obtained in the step (2) into a second-stage seed culture medium according to a proportion of 0.01-0.1% (V/V), and carrying out amplification culture to the logarithmic phase to obtain a second-stage seed solution; and (4) transferring the second-stage seed solution obtained in the step (3) into a sterilized fermentation culture medium according to the proportion of 10% (V/V,), and carrying out aerobic fermentation to obtain the erythritol. According to the method, temperature adaptive domestication culture needs to be carried out on strains from strain domestication, seed amplification culture and seed fermentation, human intervention is carried out on a thallus growth environment, the temperature in a fermentation process is increased in a stepped manner, thalli are effectively stimulated, the product amount is increased, the yield of the erythritol is increased, and the production cost is reduced.