Method for in vitro tissue culture, reproduction and preservation of Huperzia serrata

Abstract

The invention discloses a method for in vitro tissue culture, reproduction and preservation of Huperzia serrata. An optimal rapid reproduction system in the tissue culture process of Huperzia serratais established, comprising a most suitable selection method of explants, and an efficient multi-sterilization method. The key technical problems of in vitro growth, reproduction and preservation of different genotypes of Huperzia serrata are solved, and the large-scale, rapid and high-quality reproduction and preservation of Huperzia serrata within a short time are finally realized. The Huperzia serrata from different provinces and different genotypes can be reproduced in vitro, and the in vitro Huperzia serrata tissues can produce huperzine A. The method can effectively meet the growing demand for the Huperzia serrata and further reduce the damage to the wild Huperzia serrata, is conducive to the protection of the wild Huperzia serrata, and has huge scientific research value, economic value and ecological value.

Description

technical field [0001] The invention belongs to the technical fields of plant biotechnology and cell engineering, and relates to a method for culturing and propagating Huperzia serrata in vitro. Background technique [0002] Huperzia serrate (Thunb) Trev., also known as Melaleuca pagoda, Serratia serrate (Thunb) Trev., etc., is a Huperzia fern in the Huperzia family. The whole plant has the effects of detoxification, pain relief, stasis and swelling. There are two ways for Huperzia serrata under natural conditions. One is to reproduce through spores, which require a long germination period for the spores to form gametophytes underground, and it takes 6-15 years to mature from spore germination to plant maturity; Breeding is rarely found, so regeneration of wild resources is extremely limited. [0003] Since China first reported in 1972 that the alkaloid Huperzine A contained in Huperzine serrata had the effect of relaxing striated muscles in animal experiments, a large numb...

AI Technical Summary

Problems solved by technology

The chemical synthesis of huperzine A is a hotspot in chemical synthesis research, but the synthesis methods reported so far have certain limitations, and it is impossible to artificially synthesize huperzine A with high biological activity. Extracting huperzine A from plants is still is the most effective way

[0004] However, under natural conditions, it is very difficult for Huperz serrata to reproduce naturally, and it grows slowly. It takes about 15 years from spore germination to a complete plant, and the plant height is only a few centimeters to ten centimeters, which cannot meet people's expectations. The increasing demand of Huperzia serrata has led to the large-scale predatory collection of Huperzia serrata plants, and its wild natural resources are decreasing day by day, which greatly restricts the regeneration of this kind of medicinal resources, so that the artificial propagation of Huperzia serrata has become a problem. focus on

Sheng Shujun et al., Qin Daji et al. explored the cultivation and propagation system of Hupermus serrata by cutting seedlings or artificial domestication, which laid a certain technical foundation for the sustainable development and utilization of Hupernia serrata resources, but it has not yet been industrialized.

Shen Xiaoxia et al. used the tip of Hupermus serrata as explants for tissue culture, and found that it is very difficult to sterilize it. Although it has been sterilized many times, it is still difficult to achieve success.

In recent years, although Tu Yisheng's research group has obtained the prothallus of Hupermus serrata from Lushan Mountain in Jiangxi through tissue culture, it has been found that the reproducibility of the in vitro regeneration system of Hupermus serrata is still very low, and due to different regions and different genotypes The content of huperzine A in Huperzine has obvious difference

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