40results about How to "Multiply" patented technology

An engineering method for restoring the degenerated river bank by reed includes such steps as culturing reed seedlings, and transplanting them in the slope of river bank by different engineering approaches.

The artificial breeding method of wide-body golden-thread leech includes the following steps: building artificial breeding pool of wide-body golden-thread leech, seed leech selection, culturing larvaand cultivation of the wide-body golden-thread leech in large pool. Its propagation coefficient is obviously raised and stable, and is above 1:80, generally. The breeding method only has requirementsfor 2-3 years.

The invention discloses a plutella xylostella cotesia rubecula artificial propagation production method which comprises the following steps: collecting and propagating plutella xylostella cotesia rubecula species, producing hosts, conducting inoculation, propagation, development and feeding on plutella xylostella, conducting inoculation and cultivation on plutella xylostella cotesia rubecula, separating the plutella xylostella cotesia rubecula from the plutella xylostella, and conducting collecting, reserving and seed stock on plutella xylostella cotesia rubecula pupae. By means of the scheme of the plutella xylostella cotesia rubecula artificial propagation production method, advantaged plutella xylostella cotesia rubecula specie selection, propagation and pasturing technology is completed, high-quality, efficient and mass plutella xylostella cotesia rubecula propagation is achieved, mass production can be carried out, the plutella xylostella cotesia rubecula artificial propagation production method is applied to the technical field of biological control over agricultural pests, the plutella xylostella cotesia rubecula artificial propagation production method is used for preventing and treating primary pests such as the plutella xylostella on brassicaceous vegetables and meets production requirements of organic vegetables and green vegetables.

The invention discloses an intermediate aquaculture system for pinctada maxima. The intermediate aquaculture system comprises an outer ring slot and an inner slot formed by encircling the outer ring slot, wherein a communicating plate, a water level plate, a screen and a baffle are arranged in the outer ring slot; a clearance is reserved between the bottom of the communicating plate and the bottom surface of the outer ring slot; the water level plate and the baffle are plugged in the outer ring slot to form a filtering water chamber; the screen covers the top of the filtering water chamber and is inclined from the water level plate to the baffle; a sewage drain pipe is arranged at the lower end of the screen; an attachment device for attaching beneficial bacteria is arranged in the inner slot; a clean water outlet communicated with an aquaculture region is formed in the slot wall of the inner slot; water in the aquaculture region flows into the filtering water chamber after being filtered by the screen and then is extracted to the inner slot; by beneficial bacteria purification treatment, the water flows into the aquaculture region from the clean water outlet so as to realize water body circulation. The intermediate aquaculture system for pinctada maxima can be used for automatically purifying aquaculture water, so that the water quality can be kept in a good state for a long time; therefore the water change frequency is reduced, irritation to pinctada maxima stress is alleviated, and loss of bait algae along with water drainage is also reduced, so that the supply of baits is reduced, and the labor intensity is correspondingly reduced.

The invention discloses an artificial rearing, breed conservation and breeding production method for cryptolaemus montrouzieri mulsant. According to the method, a host plant type of mealybugs as efficient breeding hosts is selected; ladybirds are reared according to four stages of eggs, larvae, pupae and adults, the technology for extracting laid egg collection lures is found, and ladybird eggs are collected with the combination of the technology and a ladybird egg laying mechanism; the proper rearing technology, temperature and humidity conditions and storage conditions of the ladybirds in different stages are raised; the adults are packaged through boxes, the larvae and the eggs are packaged through paper bags, and therefore field release, application and popularization are facilitated. By the adoption of the method, the cryptolaemus montrouzieri mulsant is bred in high quality and efficiency and in large batches and the requirement for production is met.

The invention relates to a salt and alkali decomposing biological bacterial fertilizer. The salt and alkali decomposing biological bacterial fertilizer is prepared through the following components by mass percentage: 65 to 80% of humic acid, 1 to 4% of EM bacteria, 5 to 15% of nitrogenous fertilizer, 5 to 11% of phosphatic fertilizer, and 5 to 11% of potash fertilizer. The salt and alkali decomposing biological bacterial fertilizer is prepared by the following steps: (1) mixing and uniformly agitating humic acid and EM bacteria, and fermenting for 72 to 96 hours at a temperature of 20 to 30 DEG C until the number of beneficial bactera in the mixture per kg is less than 20 million, thus obtaining a fermented material; and (2) adding nitrogenous fertilizer, phosphatic fertilizer and potash fertilizer to the fermented material obtained in the step (1); and uniformly mixing to obtain the salt and alkali decomposing biological bacterial fertilizer. The salt and alkali decomposing biological bacterial fertilizer is subjected to site-directed experience of three years in Ninghe County, Tianjin; the salt and alkali decomposing biological bacterial fertilizer added to the soil of which the pH (Power Of Hydrogen) is below 10 can decrease the pH of the soil by about 0.8 averagely each year; and after the salt and alkali decomposing biological bacterial fertilizer is used in three consecutive years, the pH of soil can reach a normal level, and as a result, the rate of survival of crops is greatly increased.

The invention relates to a preparation method of a semiconductor wafer cutting blade material. In the preparation method, high-purity green silicon carbide and black silicon carbide are adopted as raw materials, and a wafer cutting blade material is prepared through the steps of jaw crushing and sifting, wet ball milling classification, pickling, overflow size separation, concentrating and dewatering, drying, blending, fine screening and the like. Silicon carbide blade materials prepared through the method are most in equiareal shape and keep sharp edges and strong cutting power, can minimize TTV of a silicon wafer, and have strong adaptability to cutting fluid such as polyethylene glycol and the like because blade material particles have larger specific area and clean surfaces. Products crushed by a wet ball mill are most in equiareal shape, have high output, and can avoid excessive crushing. The preparation method of the invention can reach over 50% of rate of finished products, and products milled with the technology have more uniform size and distribution of particles, thereby better cutting effect to silicon wafers can be achieved.

The invention discloses a method for rapidly propagating tupelos, which comprises the following steps of: a. explant inoculation; b. proliferation culture; c. root induction; and d. acclimatization and transplanting. The method facilitates the seed source retention and variation, maintains excellent traits of tupelos as well as the consistency of seedlings, has the advantages of rapid and mass superior cultivar propagation, and can produce mass tupelo seedlings in line within 6 months to 7 months.

An embodiment of the invention discloses a reservoir water eutrophication in-situ control method. The method includes following steps: step 100, removing floating garbage in polluted water by means ofartificial fishing or automatic fishing; step 200, building an artificial wetland, an ecological floating bed and submerged plants to form a three-in-one three-dimensional purifying system, and detecting quality of water in the three-dimensional purifying system to obtain a three-dimensional monitoring map; step 300, partitioning the three-dimensional purifying system according to the three-dimensional monitoring map, and isolating partitions through a three-dimensional screen; step 400, arranging a secondary purifying microorganism island shelf in a highly-polluted isolation area; step 500,performing continuous culture until purifying is finished, collecting purifying organisms, taking a water sample, and detecting water quality after coarse filtration. The embodiment further provides acontrol system, the three-in-one purifying system is formed to perform differentiated purifying on water in a targeted manner, and dual objectives of control and collection can be achieved.

The invention discloses a winter greenhouse grafting method for English hawthorn 'Scarlet' planted in Beijing area. A wild hawthorn (C.pinatifida) is selected as a rootstock and the English hawthorn 'Scarlet' is selected as a scion. The winter greenhouse grafting method includes putting the rootstock in a pot and transplanting; waking the rootstock and the scion; cutting inside and connecting in greenhouse;and managing cleft and grafted seedlings. By the winter greenhouse grafting method, the survival rates of the English hawthorn 'Scarlet' are 96.1% and 98% respectively. After a winter growth, the sprout of the grafted seedlings reaches an average length of 70cm and a thickness of 4 mm. By greenhouse grafting, the winter pruned branches of the English hawthorn 'Scarlet' are fully utilized, the grafted period is increased and the growth time of the grafted seedlings is prolonged.

The invention relates to the field of biological control of agricultural pests and aims at providing an artificial propagation and production method for diadromus collaris. The method comprises the following steps: collecting and propagating bee species, producing hosts, inoculating and propagating plutella xylostella, developing plutella xylostella pupae, inoculating the diadromus collaris, culturing and collecting the diadromus collaris, and reserving the diadromus collaris species. According to the method, the superior bee species selection, bee propagation and beekeeping technology is perfected and the diadromus collaris is subjected to high-quality efficient large-batch propagation; in addition, the large-scale continuous production can be performed, the method is applied in the field of agricultural pest control and used for preventing the plutella xylostella, the agricultural production needs are met to a great extent, and very convenience is provided for biological control of cruciferous vegetable pests.

The present invention provides a fermentation medium and a fermentation method for Enterococcus faecalis. The fermentation medium for Enterococcus faecalis comprises, by weight, 2-10 parts of yeast extract, 5-20 parts of brown sugar, 0.03-0.1 part of phosphoric acid, 0.01-0.05 part of ammonium carbonate, 1-5 parts of peptone, 0.001-0.005 part of a vitamin B complex, 0.005-0.05 part of Tween and 500-1000 parts of water. The medium does not contain water-insoluble components, so a subsequent purification step for removing the water-insoluble components is omitted; and the medium can improve thefermentation level and reduce the raw material cost by optimizing nutrient composition.

The invention relates to a rapid culture method for a peanut pod rot pathogen, which belongs to the technical field of culture methods for peanut pathogens. The method comprises the following steps: with peanut attacked by peanut pod rot as a material, screening the material by using a gradient dilution method and carrying out purification so as to obtain a peanut pod rot pathogen Calonectria sp. with high pathogenicity; transferring and inoculating the purified pathogenic pathogen onto a liquid medium by using a punching method, carrying out enrichment culture for 12 h, then coating a flat plate with an obtained enrichment liquid, carrying out dark culture and selecting a single colony in two days for subculturing; and subjecting obtained pure culture to rapid passage, selecting a bacterial strain with stable pathogenicity obtained after passage for 50 generations and preserving the bacterial strain. For a cryopreserved bacterium, a method comprising a first step of activation, a second step of enrichment and a third step of enlarged cultivation is adopted to meet a demand for large quantity in a short period in screening of anti-disease varieties of peanut. The method provided by the invention can effectively shorten culture time for fungal pathogens, realizes rapid and large-quantity culture and is of important significance to breeding of anti-disease varieties of peanut and research on to the resistance mechanism of the anti-disease varieties of peanut.

The invention discloses a method for rapid greening of exposed beach land of land-and-water ecotone. The method comprises the steps: selecting a herbaceous plant capable of breeding through creeping stems; selecting a period, when the atmospheric temperature is appropriate, the beach land is exposed and the germinating and rooting of the creeping stems are facilitated, as planting time; collecting the creeping stems of the plant in the field, chopping the creeping stems into segments according to length of internode, carrying out cultivation by using seeding nursery soil, and realizing rapid large-scale propagation by using a creeping stem breeding manner, so as to obtain plants for later use; selecting exposed boulder beaches, which are far away from the low-water-season mean water level of a river by at least 10m, of the land-and-water ecotone as quick recovery areas; and carrying out land preparation, planting and plant maintenance so as to form stable biocenosis, and then, cultivating part of plants in seeding nurseries, so as to carry out complementary planting on planting furrows and herb grid areas with silted river sand in spring next year. According to the method, the rapid greening of the exposed beach land of the land-and-water ecotone can be realized, the vegetation coverage is improved, both moisture and soil are conserved, the stable biocenosis is gradually constructed, and ecological restoration is promoted.

The invention discloses a method for rapid propagation and separation of pine wood nematodes. The method comprises the following steps: inoculating Sphaeropsis sapinea onto a PDA culture medium, carrying out culturing in an incubator with a constant temperature of 26 DEG C for 5 days, and inoculating the pine wood nematodes into culture dishes when the culture dishes are almost full of hyphae; uniformly spraying a proper amount of sterile water onto the inner surfaces of culture dish covers, adding 5 to 10 ml of sterilized water after 4 days so as to flush the culture dish covers, collecting water obtained by flushing of the culture dish covers, and carrying out centrifuging so as to be able to obtain a great amount of pine wood nematodes; after completion of collection, continuing spraying a proper amount of sterile water onto the inner surface of the culture dishes uniformly, carrying out repeated culturing for 2 to 3 times, after completion of culturing, flushing the culture dish covers with sterile water, collecting water obtained by flushing of the culture dish covers, and carrying out centrifuging so as to be able to obtain the pine wood nematodes; and slicing the culture medium into small pieces at the same time, placing the small culture medium pieces into a Baermann funnel so as to separate the pine wood nematodes from the culture medium, placing the separated pine wood nematodes into a refrigerator with a temperature of 4 DEG C, and carrying out storage for subsequent usage. Compared with a traditional culturing method, the method provided by the invention can rapidly propagate a great amount of pine wood nematodes, has simple separation method and can greatly save time.

The invention discloses a method for in vitro tissue culture, reproduction and preservation of Huperzia serrata. An optimal rapid reproduction system in the tissue culture process of Huperzia serratais established, comprising a most suitable selection method of explants, and an efficient multi-sterilization method. The key technical problems of in vitro growth, reproduction and preservation of different genotypes of Huperzia serrata are solved, and the large-scale, rapid and high-quality reproduction and preservation of Huperzia serrata within a short time are finally realized. The Huperzia serrata from different provinces and different genotypes can be reproduced in vitro, and the in vitro Huperzia serrata tissues can produce huperzine A. The method can effectively meet the growing demand for the Huperzia serrata and further reduce the damage to the wild Huperzia serrata, is conducive to the protection of the wild Huperzia serrata, and has huge scientific research value, economic value and ecological value.

The invention discloses a selective medium for culture of trichoderma harzianum. According to the selective medium, oxygen carrier powder and bamboo powder are uniformly added into a microbiological medium, then trichoderma harzianum is inoculated, and fast culture of trichoderma harzianum can be achieved. The selective medium has the oxytropic property, ultraviolet resistance, oxidation resistance, microbial decomposition resistance and other good properties, and trichoderma harzianum can breed and grow largely under the condition of sufficient oxygen; meanwhile, other contaminating microorganisms can not survive in bamboo powder, and thus obtained trichoderma harzianum is quite high in purity.

The invention discloses a sewage treatment system which comprises a plurality of serially connected sewage treatment tanks. Each sewage treatment tank comprises a water inlet, a water outlet and a carrier, and the carriers are used for providing growing environments of microorganisms in biological sewage treatment. The system specifically treats pollutant by a multistage grading treatment method and is good in treatment effect, low in expense and investment, small in occupied area, high in biological concentration, high in impact resistance, less in producing sludge, free from backflow and odor, and different sources of microorganisms exist in the carries by the aid of a stereoscopic structure.

The invention relates to the field of agricultural pest biological control and aims at providing a pupal parasitized wasp artificial breeding production method capable of enhancing drosophila biological control effects. The method comprises collecting drosophila melanogaster pupas with parasitized trichopria drosophilae perkins, performing eclosion for continuous three generations for parasitizing drosophila hydei pupas, and collecting wasp species; inoculating and breeding drosophila hydei, parasitizing and breeding the drosophila hydei pupas with queen parasite wasps, and collecting hatched parasite wasps for storage and reservation. The pupal parasitized wasp artificial breeding production method capable of enhancing the drosophila biological control effects controls the parasite time of the trichopria drosophilae perkins during production and inoculation processes, avoids low parasitic rate caused by over-short parasite time or low yield rate caused by excessive parasitism, facilitates reasonable inoculation and utilization of the parasite wasps through cultivation of the trichopria drosophilae perkins, is applicable to large-scale continuous production, optimizes dominant host selection and wasp breeding technology, achieves high-quality, efficient and large-scale propagation of the trichopria drosophilae perkins and lays a good foundation for propagation and utilization of the trichopria drosophilae perkins.

The invention relates to the field of biological control of agricultural pests and aims at providing an artificial propagation and production method for diadromus collaris. The method comprises the following steps: collecting and propagating bee species, producing hosts, inoculating and propagating plutella xylostella, developing plutella xylostella pupae, inoculating the diadromus collaris, culturing and collecting the diadromus collaris, and reserving the diadromus collaris species. According to the method, the superior bee species selection, bee propagation and beekeeping technology is perfected and the diadromus collaris is subjected to high-quality efficient large-batch propagation; in addition, the large-scale continuous production can be performed, the method is applied in the field of agricultural pest control and used for preventing the plutella xylostella, the agricultural production needs are met to a great extent, and very convenience is provided for biological control of cruciferous vegetable pests.