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Selective medium for culture of trichoderma harzianum

A Trichoderma harzianum, selective technology, applied in the field of microorganisms, can solve problems such as limited oxygen

Inactive Publication Date: 2016-12-07
北京普仁生态技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Trichoderma harzianum is widely used in many aspects, so a large number of rapid cultivation of Trichoderma harzianum is currently a difficult problem. The existing culture media for cultivating Trichoderma harzianum are some conventional microbial culture media, which can only meet the basic requirements for microbial growth. conditions, but because Bacillus subtilis is an aerobic bacterium, a large amount of oxygen is needed during the cultivation process, and the oxygen in the conventional medium is limited, so it cannot be allowed to reproduce and grow in large quantities

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A kind of selective culture medium that is used for Trichoderma harzianum culture, and its preparation method is as follows: (1) take by weighing 3 weight parts polytetrafluoroethylene, be ground into fineness and be 100 mesh powders, be dispersed in 30 weight parts (2) Weigh the quartz sand material of 3 parts by weight, evenly spray the dispersion in step 1 on the surface of the carrier material, air-dry it and then pulverize it with a pulverizer, and the obtained mixed powder is the Oxygen carrier powder; (3) After weighing 8 parts by weight of bamboo powder, 30 parts by weight of water and the oxygen carrier powder in step 2 and stirring evenly, feed saturated steam to make the temperature of the medium rapidly reach 130°C for sterilizing treatment, the time is 1min , and then rapidly cooled to 25°C, the obtained selective medium can be used for the cultivation of Trichoderma harzianum.

[0017] The selective culture medium that the present embodiment obtains is mad...

Embodiment 2

[0019] A selective culture medium for the cultivation of Trichoderma harzianum, its preparation method is as follows: (1) take 1 weight part of polytetrafluoroethylene, 2 weight parts of fluorocarbon resin, and grind it into a fineness of 100 mesh The powder is dispersed in 30 parts by weight of water to form a dispersion; (2) Weigh 3 parts by weight of quartz sand material, evenly spray the dispersion in step 1 on the surface of the carrier material, and pulverize it with a pulverizer after air-drying. The mixed powder obtained is the oxygen-carrying carrier powder; (3) after taking 15 parts by weight of bamboo powder, 30 weight water and the oxygen-carrying carrier material in step 2 and stirring evenly, feed saturated steam to make the culture medium temperature reach 130 ℃ rapidly ℃ for 1 min, and then rapidly cooled to 25 ℃, the obtained selective medium can be used for the cultivation of Trichoderma harzianum.

[0020] The selective culture medium that the present embodi...

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PUM

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Abstract

The invention discloses a selective medium for culture of trichoderma harzianum. According to the selective medium, oxygen carrier powder and bamboo powder are uniformly added into a microbiological medium, then trichoderma harzianum is inoculated, and fast culture of trichoderma harzianum can be achieved. The selective medium has the oxytropic property, ultraviolet resistance, oxidation resistance, microbial decomposition resistance and other good properties, and trichoderma harzianum can breed and grow largely under the condition of sufficient oxygen; meanwhile, other contaminating microorganisms can not survive in bamboo powder, and thus obtained trichoderma harzianum is quite high in purity.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to a selective medium for cultivating Trichoderma harzianum. Background technique [0002] Trichoderma is a kind of ubiquitous and broad-spectrum antagonistic biocontrol bacteria. Weindling reported the antagonistic properties of Trichoderma for the first time in 1932, and suggested that the fungus be used for the biological control of soil-borne plant diseases, which opened the prelude to the research on Trichoderma biocontrol. Many researchers have done a lot of research on Trichoderma, including The morphological structure, distribution, classification, biological control mechanism, fermentation production, and field application of Trichoderma have achieved remarkable results. In recent years, with the successful development of a variety of commercial Trichoderma preparations, the research on the application of Trichoderma to the biological control of plant fungal diseases ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/14C12R1/885
CPCC12N1/14
Inventor 秦泽荣王福友郭靖张迪迪王鑫龙
Owner 北京普仁生态技术有限公司
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