Rapid culture method for peanut pod rot pathogen

Abstract

The invention relates to a rapid culture method for a peanut pod rot pathogen, which belongs to the technical field of culture methods for peanut pathogens. The method comprises the following steps: with peanut attacked by peanut pod rot as a material, screening the material by using a gradient dilution method and carrying out purification so as to obtain a peanut pod rot pathogen Calonectria sp. with high pathogenicity; transferring and inoculating the purified pathogenic pathogen onto a liquid medium by using a punching method, carrying out enrichment culture for 12 h, then coating a flat plate with an obtained enrichment liquid, carrying out dark culture and selecting a single colony in two days for subculturing; and subjecting obtained pure culture to rapid passage, selecting a bacterial strain with stable pathogenicity obtained after passage for 50 generations and preserving the bacterial strain. For a cryopreserved bacterium, a method comprising a first step of activation, a second step of enrichment and a third step of enlarged cultivation is adopted to meet a demand for large quantity in a short period in screening of anti-disease varieties of peanut. The method provided by the invention can effectively shorten culture time for fungal pathogens, realizes rapid and large-quantity culture and is of important significance to breeding of anti-disease varieties of peanut and research on to the resistance mechanism of the anti-disease varieties of peanut.

Description

technical field [0001] The invention relates to a rapid cultivation method for pathogenic bacteria of peanut fruit rot, and belongs to the technical field of cultivation methods for peanut pathogenic bacteria. Background technique [0002] Peanut is an important oil crop and economic crop in my country. In recent years, peanut fruit rot, commonly known as fruit rot disease, has been increasing year after year in my country's peanut production areas, especially in the northern production areas. Fruit rot is one of the common soil-borne diseases of peanuts in the world. It is a serious hazard and difficult to control. Generally, the yield loss of diseased fields can reach about 15%, and severe disease fields can lead to crop failure. Therefore, the screening, cultivation and pathogenicity research of the pathogenic bacteria of fruit rot have become very important. The key to control. [0003] Due to the particularity of peanuts growing in the soil, the diversity of adherent ...

AI Technical Summary

Problems solved by technology

[0003] Due to the particularity of peanuts growing in the soil, the diversity of adherent and endophytic bacteria on the surface of peanuts is very high, which increases the difficulty of screening pathogenic bacteria

However, most fungi have the characteristics of long growth cycle and slow passage. Therefore, in order to quickly obtain pure cultures with stable pathogenicity, it is necessary to adopt appropriate selective culture methods and shorten the passage cycle.