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Method for determining phytophthora nicotianae virulence through in-vitro shoots

A technology of Phytophthora tabacum and Phytophthora tabacum, applied in the direction of microorganism-based methods, measurement/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of high cost, large space occupation, time-consuming, etc., and achieve pathogenicity The effect of stable performance, labor cost saving, and simple method

Inactive Publication Date: 2016-08-17
YUNNAN ACAD OF TOBACCO AGRI SCI
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Problems solved by technology

Because the mycelium block inoculates the leaves quickly, it is more suitable for measuring the pathogenicity of the bacteria, but the pathogenicity of the bacteria cannot be distinguished, and it is not suitable for the simultaneous determination of the pathogenicity and the pathogenicity of the bacteria.
The other two methods both use potted living tobacco seedlings to measure. Due to the long time for cultivating tobacco seedlings and the large space occupied, it is labor-intensive, time-consuming, and expensive, which is not conducive to large-scale experiments. The range is relatively wide, and environmental factors cause some fluctuations in the severity of the disease, and the stability and repeatability of the experiment need to be improved

Method used

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Embodiment Construction

[0010] The present invention will be further described below in conjunction with the examples, but the present invention is not limited in any way, and any transformation or substitution made based on the teaching of the present invention belongs to the protection scope of the present invention.

[0011] The method for determining the pathogenicity of Phytophthora nicotianae by isolated stems of the present invention comprises the steps of preparation of Phytophthora nicotianae hyphae block, preparation of isolated stems, inoculation, pathogenicity determination and evaluation steps, specifically comprising:

[0012] A. Preparation of Phytophthora nicotiana mycelium block: inoculate Phytophthora nicotianae onto OA medium plate, cultivate in dark at 25-28°C for 7-10 days, cut the plate mycelia into mycelial blocks;

[0013] B. Preparation of isolated stems: Select the tobacco seedlings cultivated for 60-70 days or the newly grown axillary buds cultivated for 10-15 days, and cut ...

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Abstract

The invention discloses a method for determining phytophthora nicotianae virulence through in-vitro shoots. The method comprises the following steps of 1 preparing phytophthora nicotianae sclertium; 2, preparing in-vitro shoots; 3, performing inoculation; 4, determining and evaluating the virulence. The method is simple, convenient and easy to implement, the phytophthora nicotianae virulence is expressed stably, the repeatability is good, results are reliable, and the phytophthora nicotianae virulence and virulence intensity can be accurately reflected; in actual operation, determination can be performed in batches, the method has the advantages of being small in occupied space, saving the labor cost, and shortening the experimental period, and therefore the method is suitable for being applied and popularized.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a method for measuring the pathogenicity of Phytophthora tabacum in isolated stems. Background technique [0002] Phytophthora nicotianae (Phytophthora nicotianae) is a pathogenic oomycete that causes tobacco black shank. It is seriously harmful to tobacco production and is one of the main pathogenic bacteria affecting tobacco yield and quality. At present, there are mainly three methods for determining the pathogenicity of Phytophthora tabacum: (1) Determination of pathogenicity by sticking mycelium blocks at the base of the stem of the wounded and non-traumatized tobacco seedlings (Wang Ge, Zheng Xiaobo, Lu Jiayun, etc. Yunnan Research on pathogenicity differentiation of Tobacco black shank bacteria in the province, Journal of Nanjing Agricultural University. 1997,20(4): 30-35.); (2) Determination of pathogenicity of tobacco seedlings inoculated with fungus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/645
CPCC12Q1/04
Inventor 方敦煌肖炳光刘萍花张谊寒陈学军曾建敏
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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