The invention discloses a combined 
cyclone separation / collection device and a method for separating and collecting 
pollen based on the device. The device comprises a cylindrical 
settling chamber and a double-cylinder 
cyclone. The cylindrical 
settling chamber is down communicated with an umbrella-type taker. The sidewalls of the top of the cylindrical 
settling chamber are communicated with the outer cylinder of the 
cyclone. An air duct is arranged outside the cylindrical 
settling chamber, and is communicated with the cylindrical 
settling chamber and the inner cylinder of the cyclone, respectively. Besides, the air duct is connected to an 
external variable frequency fan. A cylinder screen, a reflecting cone and a three-way air blowback 
pipe are arranged in the inner space of the cylindrical 
settling chamber. The rotating central shaft of the reflecting cone rotates through connection with an 
external variable speed motor. A stripper plate and a 
discharge chute are arranged on the bottom of the cylindrical settling chamber. Furthermore, a 
pollen collecting chamber is arranged on the bottom of the double-cylinder cyclone. The method comprises the following steps of: suctioning dust-containing 
pollen by means of an 
airflow, and filtering the dust-containing pollen; performing separation in the cylindrical settling chamber; and collecting the pollen in the cyclone, and purifying the dust-containing 
airflow for recycling. The device and the method of the invention are capable of effectively improving the pollen separation / extraction efficiency and preventing dispersion of pollen, and suitable for being popularized and used.