The invention relates to a cleaning sterilization agent of a recycled rubber. The cleaning sterilization agent comprises, by weight, 20 parts of tobacco, 8 parts of pepper leaf, 10 parts of garlic, 5 parts of soybean leaf, 10 parts of zanthoxylum bungeanum leaf, 5 parts of mulberry leaf, 5 parts of centipede, 10 parts of folium artemisiae argyi, 10 parts of ginkgo leaf, 5 parts of heartleaf houttuynia herb, 10 parts of indigowoad root, 6 parts of scutellaria baicalensis georgi, 8 parts of mariguana, 10 parts of cortex phellodendri chinensis leaf, 5 parts of common motherwort herb, 10 parts of peppermint, 5 parts of clove, 5 parts of thlaspi arvense linn, 5 parts of sophora flavescens ait, 5 parts of sophora japonica linn, 10 parts of ailanthus altissima bark, and 10 parts of bitter peach branch. During preparation, the components are weighed according to the weight ratio, crushed and mixed, water or 70-75% ethanol or ether is adopted to extract twice to obtain the extract, and drying, powder grinding and storage are performed. During use, dissolving is performed with an alcohol solution, and then 800-1200 times water spray is blended. The cleaning sterilization agent has characteristics of simple component, convenient preparation, good cleaning sterilization effect, removal of stain, oil stain and other debris on the rubber surface, rubber recycling quality improving, product processing quality improving, and effective resource utilization.