The invention relates to a plant hydroponic culture method. The method includes steps: stock plant selection, to be more specific, flushing to clean a stock plant, cutting roots, and sterilizing the plant subjected to root cutting; planting, to be more specific, planting the sterilized plant into a matrix; root promoting culture, to be more specific, subjecting the plant planted in the matrix to root promoting culture under appropriate temperature and relative humidity and preset illumination conditions, wherein in a root promoting culture process, nutrient solution is sprayed, and when root system quantity of the plant is larger than or equal to eight, and the length of each root system is larger than or equal to 5cm, root promoting culture is stopped. By the plant hydroponic culture method, utilization of expensive intelligent culture systems in a traditional hydroponic culture process is avoided. In addition, the plant hydroponic culture method is simple and feasible, the plant survival rate can be increased remarkably, production cost in a culture process can be reduced while plant root promoting time is shortened, and accordingly the plant hydroponic culture method is remarkably improved both in terms of cost and economy.