Fermentation medium and fermentation method for Enterococcus faecalis

A fermentation medium and enterococcus faecalis technology, applied in the field of microbial fermentation, can solve the problems of troublesome separation, purification and drying process, increase production cost, low fermentation level, etc. Effect

Inactive Publication Date: 2019-02-01
湖北华扬科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional Enterococcus faecalis fermentation medium formula, in order to increase the number of viable bacteria in the fermentation liquid, generally contains non-water-soluble components such as soybean meal and bran, which brings troubles to the subsequent separation, purification and drying process and increases production costs
However, the formula of serum medium based on the formula of MRS medium has high raw material cost and low fermentation level

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) Preparation of primary seed liquid

[0051]Prepare in parts by weight, weigh 1 part of glucose, 3 parts of yeast extract, and 1 part of peptone, dissolve in 95 parts of water, adjust the pH to 7.0-7.5 with sodium hydroxide, and steam sterilize at 115°C for 20 minutes. The temperature was lowered to 37° C., and 1 wt % Enterococcus faecalis stock seed solution was added. Put it in a shaker, shake the flask and cultivate it for 15-20 hours, which is the first-class seed liquid.

[0052] (2) Preparation of secondary seed liquid

[0053] According to the parts by weight, take 2 parts of yeast extract, 20 parts of brown sugar, 5 parts of bone peptone and 900 parts of water, and add them into the seed fermentation tank. Use NaOH to adjust the pH to 7.5-7.7. Steam sterilization at 115-118°C for 20 minutes. Cool down to 37°C, insert 1wt% primary seed solution, and cultivate with aeration, the ventilation rate is 0.05VVM, the stirring speed is 100r / min, the cultivation tem...

Embodiment 2

[0058] (1) Preparation of primary seed liquid

[0059] Prepare in parts by weight, weigh 1 part of glucose, 3 parts of yeast extract, and 1 part of peptone, dissolve in 95 parts of water, adjust the pH to 7.0-7.5 with sodium hydroxide, and steam sterilize at 115°C for 20 minutes. The temperature was lowered to 37° C., and 1 wt % Enterococcus faecalis stock seed solution was added. Put it in a shaker, shake the flask and cultivate it for 15-20 hours, which is the first-class seed liquid.

[0060] (2) Preparation of secondary seed liquid

[0061] According to the parts by weight, take 2 parts of yeast extract, 20 parts of brown sugar, 5 parts of bone peptone and 900 parts of water, and add them into the seed fermentation tank. Use NaOH to adjust the pH to 7.5-7.7. Steam sterilization at 115-118°C for 20 minutes. Cool down to 37°C, insert 1wt% primary seed solution, and cultivate with aeration, the ventilation rate is 0.05VVM, the stirring speed is 100r / min, the cultivation te...

Embodiment 3

[0066] (1) Preparation of primary seed liquid

[0067] Prepare in parts by weight, weigh 1 part of glucose, 3 parts of yeast extract, and 1 part of peptone, dissolve in 95 parts of water, adjust the pH to 7.0-7.5 with sodium hydroxide, and steam sterilize at 115°C for 20 minutes. The temperature was lowered to 37° C., and 1 wt % Enterococcus faecalis stock seed solution was added. Put it in a shaker, shake the flask and cultivate it for 15-20 hours, which is the first-class seed liquid.

[0068] (2) Preparation of secondary seed liquid

[0069] According to the parts by weight, take 2 parts of yeast extract, 20 parts of brown sugar, 5 parts of bone peptone and 900 parts of water, and add them into the seed fermentation tank. Use NaOH to adjust the pH to 7.5-7.7. Steam sterilization at 115-118°C for 20 minutes. Cool down to 37°C, insert 1wt% primary seed solution, and cultivate with aeration, the ventilation rate is 0.05VVM, the stirring speed is 100r / min, the cultivation te...

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PUM

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Abstract

The present invention provides a fermentation medium and a fermentation method for Enterococcus faecalis. The fermentation medium for Enterococcus faecalis comprises, by weight, 2-10 parts of yeast extract, 5-20 parts of brown sugar, 0.03-0.1 part of phosphoric acid, 0.01-0.05 part of ammonium carbonate, 1-5 parts of peptone, 0.001-0.005 part of a vitamin B complex, 0.005-0.05 part of Tween and 500-1000 parts of water. The medium does not contain water-insoluble components, so a subsequent purification step for removing the water-insoluble components is omitted; and the medium can improve thefermentation level and reduce the raw material cost by optimizing nutrient composition.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a fermentation medium and a fermentation method for Enterococcus faecalis. Background technique [0002] Enterococcus faecalis is a type of lactic acid bacteria that produces lactic acid. Enterococcus faecalis is a microbial additive listed in the Ministry of Agriculture Announcement No. 2045 "Catalogue of Feed Additives (2013)", and it is also a microorganism widely used in the animal husbandry industry. [0003] Lactic acid bacteria can regulate the intestinal health of animals and improve the immunity of animals. The fermentation process of lactic acid bacteria has always been a research hotspot in the fermentation industry. How to improve the fermentation level, reduce production costs, and thus reduce the cost of use is one of the main directions of lactic acid bacteria research. Enterococcus faecalis is a lactic acid bacterium commonly used in the animal husbandry in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 张东晓詹志春吴学军周樱吴建军凌华云张成杰
Owner 湖北华扬科技发展有限公司
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