23results about How to "Great research value" patented technology

The invention provides a preparation method and application of a biochar-based microbial inoculant, the biochar-based microbial inoculant is prepared from bacillus megatherium L2 with the preservation number of CCTCC NO: M2012381 and rice hull biochar, and the preparation method of the biochar-based microbial inoculant comprises the steps of bacillus megatherium L2 seed liquid culture and liquid fermentation, biochar proportion mixing adsorption, standing and drying. The biochar-based microbial agent prepared by the invention has a good effect of preventing and treating the tea tree anthracnose, effectively reduces the infection ability of tea tree anthracnose related pathogenic bacteria, can also effectively recruit beneficial microorganisms such as chloromentholum, actinomycelum and thick-wall mycolum in soil, further improves the prevention and treatment effect on the tea tree pathogenic bacteria, and has a good application prospect in the field of prevention and treatment of tea tree anthracnose. The method has huge application potential in protection and prevention of the tea trees and is beneficial to good growth of the tea trees.

The present invention discloses an intestinal microbiota Bacteroides fragilis toxin-neutralizing nanobody and its application. By deeply studying the enterotoxigenic Bacteroides fragilis and the toxin BFT secreted by it in the bodies of patients with colorectal cancer and breast cancer, nanobodies against BFT are expressed by phage display technology; nanobodies with high binding affinity to the antigen are screened out through biopanning technology; a nanobody with the ability to neutralize the toxicity of BFT, also known as Nb2.43, is obtained. The present invention expresses and optimizes to obtain a BFT nanobody protein with high affinity, stability and uniformity, opening up a new field for the treatment of colorectal cancer and breast cancer with nanobodies targeting BFT, and having good research value and application prospects.

The invention provides a cascaded gas target higher harmonic enhancement device and method based on Gouy phase regulation and control. According to the higher harmonic coherent amplification method based on Gouy phase regulation and control and adopting the multiple cascaded gas targets, a cascaded gas target module scheme is adopted, and the Gouy phase of specific-order gas higher harmonics is regulated and controlled by regulating and controlling the relative distance between the multiple gas targets, so that the high-order gas higher harmonics are amplified. And finally, gas higher harmonics, especially specific-order harmonics, are enhanced. The method is not limited by driving laser single pulse energy, and the energy of the generated attosecond pulse sequence can be improved. Compared with a traditional multi-gas-target scheme, the method has the advantages that the phase of specific-order gas higher harmonics can be accurately regulated and controlled, enhancement of different-order harmonics is achieved, and help is provided for research in the fields of ultrafast electron dynamics, attosecond nonlinear physics and the like.

This invention discloses a methanogenic lyase homologous to PeiR lyase and its applications. The PeiR lyase protein, targeting the peptide bonds of the methanogenic cell wall, participates in the biological process of hydrolyzing the archaea cell wall, effectively killing methanogens and reducing methane production. Proteins homologous to this lyase possess potential methane-reducing potential. This invention utilizes a series of bioinformatics software to identify protein sequences encoded by methanogenic viruses from a large amount of rumen microbial virome sequencing data, and analyzes the structural homology between these proteins and PeiR. Ultimately, this invention successfully obtained a series of methanogenic lyases homologous to PeiR. To achieve the expression of these lyases, this invention designed multiple primers to synthesize the target sequences and successfully expressed them in a prokaryotic expression system. In vitro gas production experiments with the crude enzyme solution showed that these lyases are effective in reducing methane production.

The application discloses a pyridine-yne-benzene-yne diaromatic yne precursor, which is the first aromatic yne precursor capable of continuously generating pyridine-yne-benzene-yne. The diaromatic yne precursor can be compatible with multiple substituted alkyl, aryl, silicon, alkoxy, halogen and other functional groups. The diaromatic yne precursor can have a high-selectivity nucleophilic reaction with oxygen, nitrogen, sulfur and other nucleophilic reagents to quickly construct carbon-heteroatom bonds, and can have a [4+2] cycloaddition reaction with diene to quickly construct double carbon-carbon bonds, so that the diaromatic yne precursor can be used for efficiently and quickly preparing multiple substituted pyridine, benzene ring and other derivatives, and has a wide application prospect.

The invention belongs to the technical field of tissue cryopreservation, discloses a serum-free tissue vitrification cryopreservation solution and application thereof, and particularly discloses a serum-free vitrification cryopreservation solution which comprises a permeable cryoprotectant, a non-permeable cryoprotectant, an energy metabolism regulator, an antioxidant and a culture medium. The tissue serum-free vitrification cryopreservation liquid provided by the invention is invented based on a vitrification preservation principle, is simple in procedure, short in time consumption and low in cost, does not need an expensive programmed cooling instrument to strictly control a cooling step, can effectively avoid damage caused by generation of ice crystals, and has a cryopreservation effect far better than that of slow quick freezing. The serum-free tissue vitrification cryopreservation liquid does not need to add unsafe components such as animal-derived serum, reduces the possibility of animal pathogen pollution, can be used for cryopreservation of tissues such as liver, kidney, skin and tumor, and has a recovery rate of more than 90%.

The utility model discloses a device for realizing continuous collapse of a cantilever pile support foundation pit in a centrifuge test, and relates to the technical field of foundation pit support, the device comprises a plurality of support piles, all the support piles are pre-buried in a simulation preset position, each support pile is provided with a preset section, and each support pile is internally provided with an accommodating cavity; according to the utility model, through the cooperation of the support piles and the support shafts, the action of automatically destroying the plurality of support piles at the same time can be realized, specifically, all the support piles are pre-buried in a simulation preset position, and if a continuous collapse experiment needs to be carried out, the continuous collapse experiment can be carried out, so that the continuous collapse experiment can be carried out. During use, each supporting shaft only needs to slide to be separated from the corresponding containing cavity, and then soil at the preset position is simulated to apply pressure to all the supporting piles so as to drive each supporting pile to be fractured from the preset fracture surface at the same time.

The invention discloses application of a metabolic marker in preparation of a product for monitoring PB neurotoxicity and the product, which can be used for early diagnosis of PB neurotoxicity and have the advantages of high sensitivity, rapidness, convenience and accurate and reliable result. The metabolic marker comprises LysoPE 20: 4 / 0: 0, creatine, SM d18: 1 / 24: 0, SM d18: 0 / 24: 1, LysoPC 18: 0, palmitoyl sphingomyelin, phosphocholine, PC 14: 0 / 22: 1, creatinine, PC 18: 3 / 16: 0, SM d18: 1 / 16: 0, sphingosine, choline, LysoPC 18: 1, L-carnitine, palmitic acid, PC 18: 0 / 20: 4, 3-hydroxy-2-octyl glutaric acid, L-acetyl carnitine, LysoPC 20: 4, LysoPC 16: 0, and PC 20: 1 / 14: 1.

The application discloses a biochar-mediated polyethylene microplastic photoaging method, and belongs to the technical field of microplastic degradation. The method comprises the following steps: taking biochar as an aging medium, degrading and aging polyethylene microplastics through a photoaging light source, and the mass ratio of the biochar to the polyethylene microplastics is 1:1.5-2.5. The method can effectively improve the aging effect of the polyethylene microplastics.

The present invention belongs to the technical field of lithium-ion batteries, and provides a battery health state estimation method and system based on a pre-trained large language model. The technical solution is to fuse multi-cycle charging information to perform candidate voltage combinations to efficiently obtain candidate features. By establishing a voltage-charge capacity curve in a constant current charging stage, effective candidate features can be extracted without the need for complete charging and discharging data or the need to establish a complex mathematical model or electrochemical model. At the same time, with the help of a pre-trained large language model and model fine-tuning technology, continuous, reliable and accurate estimation of the battery health state is achieved.

The invention relates to a novel medical instrument, in particular to a multifunctional quantitative percussion hammer which has five core functions of equipment master control, strength detection, motion parameter detection, body state detection and automatic knocking. The five core devices comprise a main control chip device, a piezoresistor device, a laser ranging device, a motion parameter sensor and a steering engine. The force, displacement, speed and acceleration of deep reflection can be comprehensively evaluated, and the purpose of effective and accurate quantification of deep reflection is truly achieved from the perspective of multiple variables. In addition, the tendon is automatically beaten by a machine, errors caused by different manual forces can be eliminated, and meanwhile, the device has the characteristics of power utilization safety and electromagnetic compatibility, meets the special requirements of the clinical neurology department, does not cause the change of muscle local current, does not cause the obvious change of cell discharge, and can be evaluated and verified by an electromyogram. The method possibly has great clinical applicability and research value, and particularly has great significance in differential diagnosis of nervous system diseases.

The application discloses an antibacterial peptide designed by a generative artificial intelligence framework and targeting gram-negative bacteria and an application thereof, and belongs to the fields of artificial intelligence and biotechnology. The application designs and successfully obtains a novel antibacterial peptide by a generative artificial intelligence framework, the antibacterial peptide targeting gram-negative bacteria and having good antibacterial activity. It is verified by experiments that the antibacterial peptide MTAMP003 has good bacteriostatic capacity on E. coli, S. typhimurium, P. aeruginosa and A. baumannii, exhibits low cytotoxicity on human gastric mucosa epithelium and low hemolyticity on rabbit red blood cells, and has good application prospect and research value.

The present invention discloses a preparation method and application of targeted nanovesicles derived from cells. The intracellular nanovesicles of the present invention have a large yield, a small particle size, a narrow particle size distribution range, and have a higher encapsulation rate and drug loading rate when used as a carrier to load drugs. The distribution range and degree are wider under the vitreous cavity injection mode. For example, the loaded drug can be absorbed faster under the vitreous cavity injection form, and has very good application and research value in the medical field. In particular, the present invention gives the intracellular nanovesicles targeting, and obtains targeted intracellular nanovesicles by modifying Lamp2b to carry a targeting peptide. The nanovesicles provided by the present invention have a large yield and strong targeting, can more efficiently play a therapeutic role or a drug carrier role, and have very good application prospects and research value.

The invention belongs to the technical field of rapid detection of biochemical oxygen demand (BOD) of a water body, and particularly relates to a preparation method of a compound microbial agent. The compound microorganism bacterium agent is formed by compounding according to the bacterium content, the effective viable count of the clostridium butyricum is larger than or equal to 2.0 * 10 < 10 > cfu / g, the effective viable count of the bacillus subtilis is larger than or equal to 1.0 * 10 < 10 > cfu / g, and the compound microorganism bacterium agent has the main advantages that (1) the compound microorganism bacterium agent is suitable for a wide scene range of rapid detection of BOD in a water body; (2) the compound microorganism can realize rapid detection of BOD (Biochemical Oxygen Demand) in a water body; (3) the compound microbial agent has the advantages of higher microorganism loading capacity, higher microorganism activity, quicker and more sufficient reaction with organic substances in the water body and wider detection upper and lower limit ranges; (4) the compound microbial agent is cheap in raw materials, convenient to store and transport and simple in process; and (5) the compound microorganism has good scientific research value and good market application prospect.

This application discloses a polar fluorescent probe, its preparation, and its application. The structural formula of the polar fluorescent probe is shown in formula (I): (I). When the polarity of the environment decreases, the electrons of the probe (I) are transferred from the electron donor to the electron acceptor through a conjugated pathway, emitting red fluorescence. Experiments have shown that the probe has a high fluorescence fold (267-fold) for polarity recognition, a large Stokes shift (202 nm), and its detection is not affected by ions or pH. It also exhibits optical stability and can be used to prepare reagents for qualitative detection of polarity in living cells. Furthermore, the probe possesses a tert-butyl ester group structure, exhibiting strong structural derivatization capabilities, and thus demonstrating strong scalability and applicability.

The invention discloses a dual-release microsphere which comprises a PLGA (poly (lactic-co-glycolic acid)) microsphere loaded with a medicine and an alginic acid microcapsule, and the alginic acid microcapsule is an alginic acid microcapsule loaded with stem cell exosome or / and stem cell derived vesicles. The invention also discloses a preparation method of the dual-release microspheres, which comprises the following steps: loading stem cell exosomes or / and stem cell derived vesicles through a W / O emulsification method to prepare alginic acid microcapsules, and using strontium ions as a cross-linking agent in the preparation process; and loading the drug and the alginic acid micro-capsule into PLGA through an S / O / W emulsification method to prepare the dual-release microspheres. The invention also discloses an application of the dual-release microsphere in preparation of drugs for treating spinal cord injury. The preparation method provided by the invention is simple and easy to control; when the double-release microsphere is applied to preparation of drugs for treating spinal cord injury, the treatment substances wrapped in the inner layer and the outer layer in the double-release microsphere are released into tissues at different time to play a treatment role, and finally the repair and regeneration of the damaged tissues are promoted through the synergistic effect.

The invention discloses a method for preparing a titanium-based high-entropy amorphous alloy with high thermal stability, and belongs to the technical field of high-entropy alloy preparation. The titanium-vanadium-zirconium-niobium-tantalum-molybdenum-boron high-entropy amorphous alloy film of the invention comprises the following chemical components in molar parts: 47.3 parts of Ti, 25 parts of V, 11 parts of Zr, 14 parts of Nb, 2 parts of Ta, 0.5 parts of Mo and 0.2 parts of B. The preparation method comprises the following steps: mixing the chemical components in proportion, smelting, and obtaining a high-entropy alloy target: using a single crystal silicon wafer as a substrate, sputtering the high-entropy alloy target on the surface of the single crystal silicon wafer by a magnetron sputtering method, and obtaining the titanium-vanadium-zirconium-niobium-tantalum-molybdenum-boron high-entropy amorphous alloy film. The invention provides a method for preparing a high-entropy amorphous alloy film with high thermal stability, which solves the problem that the high strength of amorphous alloys cannot be maintained at high temperatures, and has excellent practical value and research value.

The invention relates to a polyanion positive electrode material as well as a preparation method and application thereof. The preparation method of the polyanion positive electrode material comprises the following steps: in a solvent, carrying out a hydrothermal reaction on an iron source, a phosphorus source, a sodium source, a titanium source and a carbon source, and freeze-drying the reaction product to prepare a precursor; under the protection of inert gas, the precursor is subjected to a carbonization reaction, and the polyanion positive electrode material is prepared; wherein the titanium source is selected from an organic titanium compound or a polymer of the organic titanium compound. The polyanion positive electrode material obtained by the preparation method disclosed by the invention has excellent electrochemical performance and very strong expansibility and application value.

The invention discloses lactococcus lactis with tolerance and high yield of nisin as well as a preparation method and application of the lactococcus lactis. The strain is named as Lactococcus lactis in taxonomy. The obtained strain can normally grow under the condition of the nisin with the concentration of 2800IU / mL, the yield of the nisin is increased by 15%, and theoretical basis and practical experience can be provided for subsequent development of application of excellent strains in the field of fermented food bacteriostasis.

The application belongs to the technical field of biological medicine, and relates to a BCLAF1 protein Ser564 site phosphorylation antigen peptide, a specific antibody and a preparation method and application thereof. The application identifies BCLAF1 protein Ser564 as a key phosphorylation site through SIK2 kinase screening, and designs and synthesizes a phosphorylation antigen peptide based on the site. After immunizing animals, a polyclonal antibody specifically recognizing BCLAF1 Ser564 site phosphorylation modification is successfully prepared. The antibody has high sensitivity and strong specificity, is suitable for various detection platforms such as immunohistochemistry and immunoblotting, and can be used for evaluating tumor immunotherapy efficacy. The application provides a key tool for analyzing BCLAF1 phosphorylation regulation mechanism, and lays a theoretical foundation for developing an immune combined therapy strategy targeting a SIK2-BCLAF1-CCL5 axis.

The invention belongs to the technical field of feed science and feed additives, and relates to a compound additive for laying hen feed and a preparation method of the compound additive. The compound additive for the laying hen feed is prepared from the following components in percentage by weight: 2 to 10 percent of DHA (docosahexaenoic acid), 2 to 6 percent of hexahydroxy flavone, 2 to 6 percent of herba epimedii total flavonoids, 5 to 15 percent of vegetable jute, 5 to 15 percent of vernonia esculenta, 2 to 10 percent of selenium-containing antioxidant enzyme, 2 to 10 percent of beta-carotene degrading bacteria, 2 to 10 percent of marine oligosaccharide probiotics, 5 to 15 percent of dried danbei powder, 3 to 10 percent of jerusalem artichoke polysaccharide, 3 to 15 percent of functional amino acid and 2 to 10 percent of polyphenol derivatives. The compound additive for the laying hen feed, disclosed by the invention, is added into a basic feed of laying hens, so that the effects of DNA, flavonoids, vegetable jute, vernonia esculenta, the freeze-dried powder of danbei, selenium-containing antioxidant enzyme, probiotics and the like can be fully exerted, the immunity of the laying hens is enhanced, the egg yield is increased, the egg laying interval of the aged laying hens is shortened, the economic value of the aged laying hens is improved, and the laying hens have good economic benefits. The higher-level development of the laying hen breeding industry is promoted.

The application discloses a preparation method of a drug sustained-release hydrogel support and a three-dimensional osteogenic induction application of hiPSCs. Firstly, a CHIR99021@BSA-DEX@PLGA time-sequential double sustained-release composite microsphere of a core-shell structure is prepared, then the freeze-dried GelMA powder is dissolved in a PBS solution containing a photoinitiator LAP, the CHIR99021@BSA-DEX@PLGA time-sequential double sustained-release composite microsphere is added after stirring and cooling, and a drug-loaded hydrogel precursor is obtained; the precursor is injected into a mold and irradiated by a specific wavelength light source to initiate free radical polymerization of a methacryl group into glue, and a drug-loaded GelMA porous hydrogel support is obtained after demolding, freezing and drying; the drug-loaded GelMA porous support is covalently grafted with VN polypeptide through EDC / NHS chemical coupling to obtain a VN-GelMA drug-loaded porous hydrogel support. The application simulates the signal time sequence regulation law in the embryonic bone formation process and significantly improves the osteogenic differentiation efficiency and mineralization quality by constructing a biomimetic three-dimensional osteogenic microenvironment. The VN polypeptide enhances the specific recognition of stem cells and significantly improves the three-dimensional cell compatibility. The application can reduce the dependence on exogenous induction factors and is safe, low in cost and stable in induction.

The invention belongs to the technical field of biological medicines, and relates to a BCLAF1 protein Ser564 site phosphorylated antigen peptide, a specific antibody, and a preparation method and an application of the BCLAF1 protein Ser564 site phosphorylated antigen peptide. According to the invention, a BCLAF1 protein Ser564 is screened and identified as a key phosphorylation site through SIK2 kinase, a phosphorylation antigen peptide is designed and synthesized based on the site, and after an animal is immunized, a polyclonal antibody capable of specifically recognizing phosphorylation modification of the BCLAF1 Ser564 site is successfully prepared. The antibody has high sensitivity and strong specificity, is suitable for various detection platforms of immunohistochemistry, immunoblotting and the like, and can be used for evaluating the curative effect of tumor immunotherapy. According to the invention, a key tool is provided for analyzing a BCLAF1 phosphorylation regulation mechanism, and a theoretical basis is laid for developing an immune combination therapy strategy of a targeted SIK2-BCLAF1-CCL5 axis.