In-vitro tissue culture and rapid propagation method for viburnum burejaeticum

A viburnum and tissue culture technology is applied in the field of rapid propagation of pruning viburnum in vitro tissue culture, and can solve the problems of low survival rate and high pollution rate

Inactive Publication Date: 2019-01-08
JILIN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of high pollution rate and low survival rate in the existing viburnum pruning tissue culture and realize large-scale and rapid breeding of high-quality seedlings of viburnum pruning, the present invention provides a rapid propagation method of viburnum pruning in vitro tissue culture

Method used

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  • In-vitro tissue culture and rapid propagation method for viburnum burejaeticum
  • In-vitro tissue culture and rapid propagation method for viburnum burejaeticum
  • In-vitro tissue culture and rapid propagation method for viburnum burejaeticum

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Experimental program
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Effect test

Embodiment 1

[0031] Example 1 Disinfection and primary culture of explants

[0032] Choose pruned viburnum young twigs or sprouting strips that are robust and free from diseases and insect pests as explants, take materials from the field, and bring them back to the laboratory for pre-treatment of explants: first soak in detergent for 30 minutes, and cut Remove the leaves on the young branches, leave the petiole 0.3~0.5cm, and then rinse continuously with tap water for 2h, and then enter the sterile operation room for sterilization: sterilize with 0.1% mercury for 10min, rinse with sterile water 4 times to obtain sterility Explants: Cut the germ-bearing stem segments of the sterile explants into about 2cm and inoculate them in the primary medium (the primary medium is to add 6-BA (1.0mg / L), IBA ( 0.1mg / L) sucrose (30g / L), agar powder (8~10g / L), pH value adjusted to 5.8~6.0) for primary culture to make the axillary buds germinate and grow. The culture conditions are: keep light for 14h ( Every...

Embodiment 2

[0037] Example 2 Tissue culture seedling multiplication culture

[0038] Transfer the viable shoots from the primary culture to a proliferation medium for subsequent proliferation and culture, with 14 hours of light per day (from 8:00 am to 22:00 every day), the light intensity is 2000Lx, and the culture temperature is about 25℃. . The suitable proliferation medium formula for tissue culture seedlings is to add 6-BA (2.0mg / L), IBA (0.2mg / L), GA at an appropriate concentration in MS medium. 3 (0.4mg / L), agar powder (8-10g / L), sucrose (30g / L), pH value adjusted to 5.8-6.0. After many subcultures, cluster sprouts are formed, such as figure 2 As shown, the succession cycle is about 30 days. See Table 2 for the effects of different basic media on the proliferation and growth of tissue cultured seedlings. The effects of different types and concentrations of auxin on the proliferation and growth of tissue cultured seedlings are shown in Table 3.

[0039] Table 2 The effect of differen...

Embodiment 3

[0047] Example 3 Rooting culture in bottle

[0048] Inoculate the rootless tissue culture seedlings with a height of about 2~3cm and strong growth on the rooting medium for rooting culture. The culture temperature is controlled at about 25℃, the light intensity is about 2000Lx, and the light time is from 8:00 to evening every day. Tissue cultured seedlings were induced to take root at 22:00 (14h). The best rooting medium is to add appropriate concentration of IBA (2.0mg / L), sucrose (30g / L) and agar powder (8-10g / L) to 1 / 2MS medium, and adjust the pH value to 5.8-6.0. After 15-20 days of cultivation, rooting will begin, and 3-8 thick adventitious roots can be produced within 30 days, such as image 3 As shown, the rooting rate is over 90%. The effects of different types and concentrations of hormones on the rooting of tissue cultured seedlings are shown in Table 4.

[0049] Table 4 The effects of different types and concentrations of hormones on the rooting of tissue cultured seed...

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Abstract

The invention discloses an in-vitro tissue culture and rapid propagation method for viburnum burejaeticum and belongs to the technical field of plant tissue culture. The problems that the pollution rate is high and the survival rate is low in the conventional viburnum burejaeticum tissue culture are solved. The method disclosed by the invention comprises the following steps: adopting a plant tissue culture technology, taking semi-lignified stems of viburnum burejaeticum as explants, obtaining explants with high survival rate and relatively low death rate and pollution rate by using an explantsterilization method, taking MS as a minimal medium, adding different plant hormones of appropriate types and concentrations to induce germination of axillary buds, and performing enrichment culture,rooting culture and transplanting under appropriate culture conditions, thereby obtaining lots of high-quality regenerated plants in a short time. A certain foundation is laid for tissue culture and rapid propagation of the viburnum burejaeticum, and necessary technical support is provided. The method disclosed by the invention can provide technical reference for large-scale propagation of high-quality seedlings of the viburnum burejaeticum, and has significances on comprehensive development and utilization of the viburnum burejaeticum.

Description

Technical field [0001] The invention belongs to the technical field of plant tissue culture, and specifically relates to a method for rapid propagation of pruned viburnum in vitro tissue culture. Background technique [0002] Pruning viburnum, also known as warm wood striped viburnum, is a deciduous shrub of Caprifoliaceae, Viburnum, and naked bud group. The height of the tree is 1-2m, generally not more than 5m, and the bark is dark gray. Only annual branchlets are covered with short hairs, and the paper-like oval or elliptical leaves have short hairs. Umbrella-like tidbits, white radiating corolla. The stone fruit is red in the first ripe period and black in the mature period. The seeds are oblong with grooves on the surface. The flowering period of pruning viburnum is from May to June, and the fruit maturity period is from August to September. The plant is not only for ornamental purposes, but also has edible fruits and seeds for squeezing oil, which is a kind of oil plant ...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G24/28A01G24/12
CPCA01G24/12A01G24/28A01H4/001A01H4/008
Inventor 李金英赵春莉张志东
Owner JILIN AGRICULTURAL UNIV
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