Tissue engineering material construction method based on amniotic mesenchymal stem cells

Abstract

The invention provides a tissue engineering material construction method based on amniotic mesenchymal stem cells, and relates to a stem cell tissue engineering material. The construction method comprises: 1) obtaining and separating fresh human amnion, 2) carrying out in situ amplification on the obtained amniotic mesenchymal stem cells, 3) adopting different cell differentiation culture mediums to induce differentiation of the amniotic mesenchymal stem cells toward cells with specific types, and 4) constructing tissue engineering materials with different forms. According to the present invention, the in vitro tissue culture technology is adopted to carry out in situ amplification on the amniotic mesenchymal stem cells, the differentiation of the mesenchymal stem cells toward nerve cells, liver cells and other types of the cells is induced, and the tissue engineering material having the corresponding cell type is constructed, and can be used for corresponding disease treatments.

Description

technical field [0001] The invention relates to a stem cell tissue engineering material, in particular to a method for constructing a tissue engineering material based on amniotic mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells widely exist in various adult tissues. A large number of studies have confirmed that mesenchymal stem cells can differentiate into a variety of somatic cells and can be used for clinical treatment of various diseases. At present, people mainly obtain mesenchymal stem cells from bone marrow, fat and other tissues, but there are some defects: either the process of obtaining stem cells causes mechanical invasive damage to the body (such as bone marrow), or the obtained stem cells have limited proliferation and differentiation ability ( such as fat), or insufficient sources of material (such as dental pulp). These defects limit the clinical application of mesenchymal stem cells. [0003] The amniotic membrane is the innermos...

AI Technical Summary

Problems solved by technology

At present, people mainly obtain mesenchymal stem cells from bone marrow, fat and other tissues, but there are some defects: either the process of obtaining stem cells causes mechanical invasive damage to the body (such as bone marrow), or the obtained stem cells have limited proliferation and differentiation ability ( such as fat), or an insufficient source of material (such as dental pulp)

One is that mesenchymal stem cells have left their original microenvironment, and their stem cell characteristics are prone to change during in vitro expansion, leading to terminal differentiation of cells; the other is that after mesenchymal stem cells are expanded in single-cell culture, if For in vivo transplantation, it is often necessary to further construct tissue engineered products, such as bone tissue, cartilage tissue, muscle tissue, tendon tissue, nerve tissue, etc., which pose great difficulties to the application of such cells

So far, there have been no reports on the successful construction of tissue engineering products derived from amniotic mesenchymal stem cells

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