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Method for extracting mesenchymal stem cells from pluripotent stem cells of people or animals

A pluripotent stem cell and mesenchymal technology, applied in the field of cell biology, can solve the problems of rapid aging, low purity, and low yield of mesenchymal stem cells, and achieve the effect of solving the quality difference and strengthening the proliferation and differentiation ability.

Inactive Publication Date: 2012-01-18
深圳市诗得美生物医学科技发展有限公司
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Problems solved by technology

[0004] The invention provides a method for extracting mesenchymal stem cells from human or animal pluripotent stem cells, so as to solve the problems of low output rate, small yield, low purity, fast aging and inter-batch quality of mesenchymal stem cells existing in the prior art unstable problem

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  • Method for extracting mesenchymal stem cells from pluripotent stem cells of people or animals
  • Method for extracting mesenchymal stem cells from pluripotent stem cells of people or animals
  • Method for extracting mesenchymal stem cells from pluripotent stem cells of people or animals

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Embodiment Construction

[0026] In order to better understand the technical solution of the present invention, the following describes the embodiments provided by the present invention in detail.

[0027] An embodiment of the present invention provides a method for extracting mesenchymal stem cells from human or animal pluripotent stem cells, such as figure 1 As shown, it includes the steps:

[0028] A. Based on the sampling of human or animal pluripotent stem cells, the pluripotent stem cells include: embryonic stem cells (Embryonic Stem Cells, ESC) and artificially induced pluripotent stem cells (induced Pluripotent Stem Cells, iPSC), etc., using enzyme digestion solution to sample The sample is digested, wherein the enzyme digestion solution includes neutral protease and type IV collagenase;

[0029] B. Adding mesenchymal stem cell conditioned medium to the isolated single cells, inoculating and culturing the cells;

[0030] C. Adhesive purification is performed on the cultured cells, and the mes...

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Abstract

The invention provides a method and a medicine for extracting mesenchymal stem cells from pluripotent stem cells of people or animals. The method comprises the following steps of: A, sampling on the basis of the pluripotent stem cells of the people or the animals, and digesting a sample by using an enzymatic digestive fluid, wherein the enzymatic digestive fluid comprises neutral protease or type IV collagenase; B, adding a conditioned culture solution of the mesenchymal stem cells into separated single cells, performing cell vaccination and culturing; and C, performing wall-sticking purification on cultured cells, and obtaining the mesenchymal stem cells through magnetic bead sorting. The problems of low output rate, low yield, low purity and quick aging of the mesenchymal stem cells as well as unstable interassay quality of the cells in the prior art are solved.

Description

technical field [0001] The invention relates to the technical field of cell biology, in particular to a method for extracting mesenchymal stem cells from human or animal pluripotent stem cells (Pluripotent Stem Cells). Background technique [0002] Mesenchymal stem cells (MSCs) are a type of tissue stem cells with multi-differentiation potential and self-renewal ability, which can differentiate into various tissue cells: osteoblast, cartilage, fat, blood vessel, nerve cells, etc. Mesenchymal stem cells were first isolated from bone marrow. In recent years, mesenchymal cells with stem / progenitor cell characteristics have been isolated from bone marrow, peripheral blood, compact bone, cartilage, muscle and other tissues. The common feature of these tissues is that they are composed of mesenchyme and blood vessels derived from extraembryonic mesoderm. In terms of differentiation ability, mesenchymal stem cells can differentiate into various tissue cells: osteoblast, cartilage,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K35/12A61P37/02A61K35/28
Inventor 连祺周
Owner 深圳市诗得美生物医学科技发展有限公司
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