Isolated culture method of human umbilical cord mesenchymal stem cells

A quality stem cell, separation and culture technology, applied in the field of stem cells and regenerative medicine, can solve problems such as difficulty in meeting large-scale experimental research and clinical applications, prolonging the time of in vitro separation of cells, and affecting cell activity, so as to ensure cell proliferation, differentiation, shortening Enzyme action time, the effect of strong proliferation and differentiation ability
CN112646775AInactive Publication Date: 2021-04-13THE PEOPLES HOSPITAL WEIFANG CITY CN0
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THE PEOPLES HOSPITAL WEIFANG CITY CN0
Publication Date
2021-04-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an isolated culture method of human umbilical cord mesenchymal stem cells. The isolated culture method comprises the following steps of: collecting storage and transportation umbilical cords, separating Wharton's jelly tissues, extracting and culturing cells, and carrying out cell passage and breed conservation cryopreservation. According to the isolated culture method disclosed by the invention, umbilical cord adventitia and blood vessels are removed to obtain pure Wharton's jelly tissues, so that the purity of the isolated mesenchymal stem cells is higher and the quantity of the isolated mesenchymal stem cells is larger; a two-step enzyme digestion method is utilized, so that the preparation period of primary cells is greatly shortened; and in addition, the cells are good in activity and strong in multiplication capacity, have good differentiation potential and high separation efficiency, and especially can meet the cell requirements of large-scale experimental research and clinical application.
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Description

technical field

[0001] The invention belongs to the field of stem cells and regenerative medicine, relates to the technology of separating and culturing stem cells, in particular to a method for separating, extracting and culturing mesenchymal stem cells from human umbilical cords. Background technique

[0002] Mesenchymal stem cells are a special type of stem cells with strong self-renewal and multidirectional differentiation potential. Mesenchymal stem cells have received great attention in various fields of medicine because of their great potential in immune regulation and tissue repair. Mesenchymal stem cells can be obtained from a wide range of sources, such as bone marrow, synovium, fat, umbilical cord blood, umbilical cord and other tissues. In addition, after special induction, it can differentiate into various tissue cells such as bone cells, endothelial cells, chondrocytes, cardiomyocytes, and adipocytes. At present, the main research source of mesenchymal stem c...

Claims

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