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Induction method of megakaryocyte progenitor cells

A technology of megakaryotic progenitor cells and cells, which is applied to animal cells, vertebrate cells, blood/immune system cells, etc., can solve the problems of low differentiation and amplification efficiency, achieve less ethical disputes, lower chances of infection and transmission, The effect of improving amplification efficiency

Active Publication Date: 2018-12-18
HUZHOU COLLEGE +1
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Initially, umbilical cord blood CD34+ cells or Lin- / CD126- cells were expanded mainly by adding different combinations of cytokines in vitro, but the cytokines added in vitro need to be continuously supplemented to maintain cell culture for a long time. and low amplification efficiency

Method used

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  • Induction method of megakaryocyte progenitor cells
  • Induction method of megakaryocyte progenitor cells
  • Induction method of megakaryocyte progenitor cells

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Embodiment Construction

[0046] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0047] combine figure 1 , describe in detail the method for inducing megakaryotic progenitor cells of the present invention, such as figure 1 As shown, it includes the following steps:

[0048] S11: Separation and screening of cord blood CD34+CD126- cells;

[0049] S12: Isolate and culture umbilical cord mesenchymal stem cells;

[0050] S13: Cord blood CD34+CD126- cells were co-cultured with umbilical cord mesenchymal stem cells to expand and induce differentiation of megakaryotic progenitor cells.

[0051] The method for inducing megakaryotic progenitor cells in this example is to induce and differentiate mega...

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Abstract

The invention discloses an induction method of megakaryocyte progenitor cells. The method includes: isolating and screening cord blood CD34+CD126- cells; isolating and culturing umbilical cord mesenchymal stem cells; co-culturing the cord blood CD34+CD126- cells with the umbilical cord mesenchymal stem cells, and conducting amplification and induced differentiation of megakaryocyte progenitor cells. The induction method of megakaryocyte progenitor cells provided by the invention performs isolation and screening of cord blood CD34+CD126- cells and adopts the way of co-culture with the umbilicalcord mesenchymal stem cells for induced differentiation of megakaryocyte progenitor cells, increases the induced differentiation efficiency of in vitro megakaryocyte progenitor cells, and also enhances the amplification efficiency.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for inducing megakaryotic progenitor cells. Background technique [0002] At present, the main clinical treatment for patients with severe thrombocytopenia is transfusion of donated platelets, but there are defects such as short shelf life of platelets, harsh storage conditions, repeated transfusions, easy to produce platelet antibodies, and increased risk of blood-borne diseases, which seriously restrict the use of platelets. Clinical application. Inducing the differentiation of umbilical cord blood hematopoietic stem cells into megakaryocytes in vitro opens up new research ideas for broadening the source of platelets. [0003] As another rich source of hematopoietic stem cells besides bone marrow and peripheral blood, umbilical cord blood has the advantages of easy collection, low immunogenicity and high expansion efficiency. Megakaryotic progenitor cells from di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0789
CPCC12N5/0647C12N2502/137
Inventor 徐志国钮移坤郝超刘超杨旭巍任振辉肖舒瑜
Owner HUZHOU COLLEGE
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