Porous chamber for tissue culture in vivo
a tissue culture and porous chamber technology, applied in the field of tissue culture in vitro, can solve the problems of difficult to overcome the seeding of cells, the loss of original cells and functions, and the burden on families and societies
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[0025]The method for cartilage tissue culture in vitro:
[0026]1. Preparing the Biologically Absorbable Porous Chamber 10 Having hollow Cavity 2
[0027]The dissoluble polymer material chosen in this embodiment is the PLGApolymer prepared through ring scission polymerization (the molecular weight of polymer is approximately 200,000). By crushing the block-shaped PLGA polymer material in the crushing machine, the polymer particles with diameters ranging from 177 to 250 μm are to be obtained after sifting through the sifting net having 60 to 80 meshes. The water-soluble material chosen to add in the scaffold for creating the porous structure is sodium chloride particles with the diameters of approximately 250 μm; acetone is chosen to be the organic solvent used for dissolving polymer particles. The PLGA polymer particles and the sodium chloride particles, based on the weight ratio of 10 / 90, are to be well-proportionally mixed through way of spinning, following which the PLGA polymer partic...
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