A method for creating germplasm resources of disease-resistant and high-quality head cabbage

Abstract

The invention discloses a method for creating germplasm resources of disease-resistant and high-quality head cabbage, which belongs to the technical field of cabbage breeding. This method selects the CMS male sterile line / maintainer line of cabbage with excellent comprehensive traits to be crossed with fertile cabbage plants with excellent comprehensive traits, and then adopts in vitro tissue culture from the hybrid offspring to recombine and screen for similar spherical shape, similar ripening period, and color. The sterile lines with similar comprehensive traits are backcrossed with similar fertile plants for 3 generations, and the fertile plants are self-crossed for one generation and then interplanted to breed new CMS male sterile lines with different ripeness, spherical shape and excellent color comprehensive traits / Maintainer lines, and other new excellent fertile strains. Through the improved culture of callus protoplasts, the frequency of protoplast regenerated plants can be increased, chimeras and polyploids can be eliminated, excellent fertile plants can be retained, and more and better new germplasm resources can be created.

Description

technical field [0001] The invention belongs to the technical field of cabbage breeding, and in particular relates to a method for creating germplasm resources of disease-resistant and high-quality head cabbage. Background technique [0002] Heading cabbage (Brassica oleacea L.var.capitata L.) is a variety of Brassica plants in the family Brassica, also known as cabbage, cabbage, etc. It originated from the Mediterranean coast and has a mild climate. It is the main vegetable crop in China. One of them was introduced into China in the 16th century. Heading cabbage (hereinafter referred to as cabbage) has the characteristics of disease resistance, strong adaptability, easy storage and transportation, high yield, and good quality. It is widely cultivated in various parts of our country and has developed rapidly. One of the main vegetables in summer, autumn and winter, with an annual planting area of ​​3 million hectares 2 All of the above plays an important role in the annual...

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Problems solved by technology

However, the use of self-incompatible lines for seed production requires a lot of manpower and time for parental breeding (through artificial pollination at the bud stage), which is very costly, and the promotion area is limited by the number of seeds produced.

What's more unfavorable is that the long-term continuous self-trading viability of the parents degrades, so that some excellent characters are lost, and the hybridization rate is difficult to reach 100%. However, when the cytoplasmic male sterile line (CMS male sterile line) is used to prepare excellent varieties of cabbage, the hybridization rate reaches 100%. %, do not need the restoration line, only the male parent line

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