Method for endangered Chinese azalea in vitro tissue culture propagation and storage

Abstract

The invention discloses a method for endangered Chinese azalea in vitro tissue culture propagation and storage. The best rapid propagation system and storage system are built in the Chinese azalea tissue culture process. According to the method, an optimum explant selection method, an efficient multiple sterilization method, optimum culture medium components, adventive bud rooting, acclimatizating and transplanting condition control for Chinese azalea in vitro growth propagation and storage and other key technological problems are included, and finally large-quantity, rapid and high-quality propagation of Chinese azalea in a short time is achieved. The Chinese azalea test tube plantlet differentiation rate can reach 100%, the adventive bud growth coefficient month proliferation rate can reach 8-10.5, and the rooting rate and the acclimatizating survival rate are 85% or above after 40 d, so that the increasing requirement of people for Chinese azalea can be effectively met, then, damage by people to wild Chinese azalea is reduced, protection of the endangered wild Chinese azalea is facilitated, and huge scientific research value, economic value and ecologic value are achieved.

Description

technical field [0001] The invention belongs to the technical field of plant biotechnology and cell engineering, and relates to a method for in vitro tissue culture, reproduction and preservation of endangered sheep deer. Background technique [0002] Rhododendronmolle (Rhododendronmolle G.Don), also known as Naoyanghua and Balima, is the only native species in the subgenus Rhododendronmolle in China. It blooms in spring and has bright yellow flowers. It can be used for viewing. It is a rhododendron molle color variety. An important resource for improvement. In addition, the organs of the whole plant of the goat roe contain various active ingredients, and the flowers, roots, and fruits are all available for medicinal purposes. Its extracts can treat rheumatoid, febrile malaria, chronic glomerulonephritis, and hypertension. It is used as an anesthetic and analgesic in industry, and used as an insecticide in agriculture. It has a very good killing effect on schistosomiasis an...

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for in vitro tissue culture, reproduction and preservation of endangered sheep roe deer, aiming at the sharp reduction in the area and population of sheep roe deer distributed in the wild, and the small seeds are difficult to germinate under natural conditions, and artificially Propagation by cuttings or divisions is also difficult to survive, there are explant sterilization difficulties in the tissue culture process, the acquisition rate of sterile explants is low, and the explants are difficult to differentiate and multiply. The present invention provides an endangered The method of in vitro large-scale reproduction and preservation of sheep roe deer provides technical support for realizing large-scale, rapid and high-quality reproduction of sheep roe deer in a short period of time, and provides tissue culture seedlings to meet people's growing demand for sheep roe deer, Provide technical support and reference for the protection of wild endangered sheep roe

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