Method for increasing production efficiency of hybrid maize seeds

Abstract

The invention disclose a method for increasing the production efficiency of hybrid maize seeds. The method includes the steps of S1, culturing maize seeds to obtain maize calluses; S2, separating themaize calluses into a maize protoplast solution; S3, removing the supernate of the maize protoplast solution, and performing precipitation and resuspension; S4, adding maize protoplast into PEG1000, slowly adding 9.5ml of Hanks solution into the test tube 90 seconds later, standing the test tube in a water bath pot, centrifuging and abandoning supernate, adding 1ml of cell culture liquid into thecell precipitates, gently evenly mixing, and culturing in water bath of 37 DEG C for 30 minutes to obtain activated maize protoplast fused cells; S5, using a plant in-vitro tissue culture method to culture the maize protoplast fused cells into regenerated hybrid maize plants. The method has the advantages that maize seeds are bred through a somatic hybridization method, breeding cycle is shortenedgreatly, breeding quality is increased, breeding time is shortened, and the targeting performance of breeding objects is increased.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a method for improving the production efficiency of corn hybrid seeds. Background technique [0002] Commercial seed production is a complex process involving many steps. In the case of corn, inbred plants must first be crossed in the field under conditions that allow male pollen to fertilize the silk on the female ear in order to produce the seed that will be sold to farmers. Seeds, but now corn seed hybrids are all pollinated by female flowers in the field. The pollination time is long, and the production cycle to produce a stable hybrid seed is long. [0003] Therefore, we propose a method to improve the production efficiency of maize hybrid seeds to solve the above problems. Contents of the invention [0004] The purpose of the present invention is to solve the shortcoming of long growth cycle of stable corn hybrid seeds in the prior art, and propose a method for im...

AI Technical Summary

Problems solved by technology

[0002] Commercial seed production is a complex process involving many steps. In the case of corn, inbred plants must first be crossed in the field under conditions that allow male pollen to fertilize the silk on the female ear in order to produce the seed that will be sold to farmers. seeds, but now corn seed hybrids are pollinated by female flowers on the field. This kind of pollination takes a long time, and the production cycle to produce a stable hybrid seed is long.