38 results about "Somatic Cell Hybridization" patented technology

The provided specific site can integrates exogenous gene to stable express and affect little to the acceptor. Besides, it also provides a composite gene structure, which can be integrated by rice gene DNA sequence near specific site and DNA sequence of exogenous gene, and be used to culture new rice series by sexual hybridization and somatic hybridization.

Detection of mutations associated with hereditary diseases is complicated by the diploid nature of mammalian cells. Mutations present in one allele are often masked by the wild-type sequence of the other allele. Individual alleles can be isolated from every chromosome within somatic cell hybrids generated from a single fusion. Nucleic acids from the hybrids can be analyzed for mutations in an unambiguous manner. This approach was used to detect two cancer-causing mutations that had previously defied genetic diagnosis. One of the families studied, Warthin Family G, was the first kindred with a hereditary colon cancer syndrome described in the biomedical literature.

The invention belongs to the technical field of genetic engineering for rice, and particularly relates to a method for cultivating insect-resistant transgenic rice T1c-19. The method includes transferring target genes into excellent rice restorer lines minghui 63 by the aid of synthesized novel insecticidal genes cry1C, bar genes and agrobacterium tumefaciens mediated genetic transformation processes to obtain novel transgenic rice materials T1C-19 with high rice Lepidoptera insect resistance. The novel insecticidal genes cry1C are shown as SEQ ID NO:1 and are used as the target genes, and the bar genes are used as selectable marker genes for transferring the target genes into the excellent rice restorer lines minghui 63. The method has the advantages that the insect-resistant genes of T19-1 further can be recombined into novel rice germ plasma by the aid of sexual hybridization and somatic hybridization technologies, flanking sequences of insertion fragments of exogenous genes of transgenic plants of the rice are 5'-end flanking sequences shown as SEQ ID NO:3 and or 3'-end flanking sequences shown as SEQ ID NO:5, and the method for completely cultivating novel insect-resistant varieties (strains) of rice is provided; the T1C-19 is a novel genetic resource.

The invention discloses a rubber tree protolast culture plant regeneration method which comprises the following steps of: establishing an embryonic cell suspension system, separating and purifying protoplast, nursing and culturing the protoplast, carrying out mulitiplication in protoplast regeneration cell clone, inducing somatic embryos and regenerating plants. The rubber tree protolast culture plant regeneration method disclosed by the invention is simple and easy to carry out, the components of a nursing and culture medium employed to nursing and culturing the protoplast are greatly simplified, and the suspension cell systems of the varieties of the rubber tree self are used as the nursing cells, so that the production cost is greatly lowered, the efficiency that the rubber tree protolast is split to form cell clone and the efficiency that the somatic embryos are generated subsequently are effectively improved. A good receptor system is provided for rubber tree variety improvement and quality innovation by utilizing biotechnological breeding modes with significant application prospects, such as protoplast fusion (namely somatic hybridization) and genetic transformation.

The invention discloses a seaweed breeding method based on an induction polyploid gametocyte, which is characterized by comprising the following steps: firstly processing haploid seaweed gametophyte cells by utilizing mitosis hinder agent to lead the chromosome in the cells to be doubled, so as to induce the haploid seaweed gametophyte cells into polyploid seaweed gametophyte cells, and then breeding polyploid seaweed thalluses by utilizing a seaweed gametophyte cell hybridization technique. The method of the invention has the advantages that the seaweed gene content can be improved through polyploid breeding; and the seaweed physiological activity can be strengthened by the increased quantitative character gene, so as to improve economic character thereof. The chromosome doubling method of the invention can be applied for breeding homology polyploids and allopolyploids.

The process of transferring Tibetan medicinal effective component into receptor cell by means of somatic cell cross breeding technology includes the following main steps: 1) selecting parent material gene types; 2) dissociating the receptor protoplaster; 3) UV irradiating donor protoplaster; 4) fusing the donor protoplaster and the receptor protoplaster and culturing; 5) identifying hybrid callus and regenerated plant; and 6) detecting target characteristic in somatic cell hybrid. The present invention obtains cell line with high medicinal component content and fast growth by means of somatic cell cross breeding technology and may be used in obtaining famous and rare Tibetan medicinal effective component.

The invention belongs to the technical field of cell culture, and relates to a catalpa bungei embryonal cell suspension culture method, wherein the method comprises a step of taking embryos of immature fruits of catalpa bungei as explants, and sequentially comprises the steps of inducing embryonal callus, inducing an initial suspension culture product, establishing an embryonal cell suspension system, and regenerating plants; the induction culture medium of the initial suspension culture product is 1 / 2MS; and, the culture solution of the catalpa bungei embryonal cell suspension system is that polyethylene glycol (PEG) 6000 is added based on the 1 / 2MS culture medium. By means of the method disclosed by the invention, the steady catalpa bungei embryonal cell suspension system can be established; furthermore, the plantlet survival rate of embryo regeneration plants is high; emerged seedlings are tidy and normal; and the method disclosed by the invention can provide a steady material source and a platform for catalpa bungei embryo development, somatic hybridization, genetic transformation, and storage and research of plant germplasm resources, and provide important theoretical and experimental basis for industrial breeding of a bioreactor technology based on suspension cell culture.

The invention relates to an isolated culture method for peony ovules. After peony ovaries which are fertilized for 15 to 90 days are disinfected by mercuric chloride, the ovules are taken out and divided into a 15-to-48-day ovule group and a 49-to-90-day ovule group; the 15-to-48-day ovule group is inoculated into MS culture medium, cultured in the dark for 3 to 15 days, then inoculated into MS culture medium II, cultured in the dark for 10 days and then cultured in light for 7 to 27 days; afterwards, the 15-to-48-day ovule group is inoculated into the MS culture medium II again and cultured in light for 8 to 28 days, so that mature peony seeds I are obtained; and after being first cultured in the dark for 10 days, the 49-to-90-day ovule group is cultured in light for 7 to 27 days, then inoculated in the MS culture medium II again and cultured in light for 8 to 28 days, so that mature peony seeds II are obtained. The isolated culture method for peony ovules can be used in the isolated research on peony embryo development, and solves the problems of the early abortion of the embryos of good-looking peony varieties and the abortion of distant somatic hybrid peonies, and by way of ovule culture, the improved research on peony varieties can be carried out as well.

The invention belongs to the technical field of plant somatic cell hybridization, in particular to a method for sugarcane somatic cell hybridization, comprising the following steps: (1) preparation of parental protoplasts; (2) passivation of protoplasts; (3) protoplast fusion induction The addition of thing: wherein a kind of parent protoplast is cultured with silicate aqueous solution, mixes with another kind of parent protoplast equal volume to obtain mixed protoplast; (4) fusion of protoplast: the step (3) obtains Mix protoplasts for electrofusion. The invention utilizes the principle that silicon has a high affinity with glycolipids on the surface of plant cell membranes. The two parental protoplasts are fused under the induction of silicon to increase the one-to-one fusion rate. The hybrid offspring also has high yield and high sugar in ROC22 sugarcane , high drought tolerance genes, and high sugar cold tolerance genes of GT28 sugarcane.