Sterile line rapid transformation breeding method

Abstract

The present invention discloses a sterile line rapid transformation breeding method, and sterile line rapid transformation breeding is performed through combining of somatic cell hybridization and molecular marker selection. The sterile line rapid transformation breeding method belongs to the technical field of crop breeding. First, donor (sterile) cytoplasm and receptor (fertile) protoplast are separated; then iodoacetamide (IOA) is used for processing the receptor protoplast for inactivation of the cytoplasm, then the donor cytoplasm and receptor protoplast are mixed in the ratio of 2: 1 (v / v ), an electric fusion method or a chemical fusion method is used for donor and receptor fusion; and finally, under appropriate culture conditions, the fusion body is regenerated, a sterile regenerated plant with genotype and phenotype consistent with that of the receptor is selected, and the plant which can seed normally after pollinated by receptor pollen is a receptor sterile line.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows for better control over how crops grow or develop without requiring much human effort or resources. Its benefits include shorter cycles with fewer generations needed, less space required by male fertilizers that last longer than usual, increased yield from seedlings due to their faster growing process, improved quality through regular pollination processes, and enhanced combination capabilities when combined together into one crop variety called tomato (Solanum lycopersicum).

Problems solved by technology

This patented describes how men cannot produce seeds due to their ability to reproduce themselves naturally or accidently without being able to control them effectively during breeding cycles. Current techniques involve manually removing unwanted parts from an already grown part called stamens that needs to be transferred later on when new ones start growing again. However this process may require upwards of three months' time before successful reproduction occurs. Additionally current methods often result in low yields because they rely heavily upon human effort and materials resource allocation.

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