Method for genetic transformation of soybean chloroplast

A technology of genetic transformation and chloroplast, applied in the field of agricultural planting, can solve the problems of small number of chloroplasts, difficulty in transformation, etc., and achieve the effect of normal growth and development
CN103290044AInactive Publication Date: 2013-09-11JILIN ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JILIN ACAD OF AGRI SCI
Publication Date
2013-09-11
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention discloses a method for genetic transformation of soybean chloroplasts, belonging to the technical field of agriculture planting. On the basis that multiple factors such as target material treatment, gene gun bombardment parameters, screening pressure and screening period are optimized, homogeneous chloroplast transgenic soybean plants are ultimately obtained by sufficiently utilizing the characteristic that soybean embryonic somatic cell clusters can be continuously multiplied, by carrying out appropriate natural dehydration treatment on a target material and by carrying out continuous, multiple and high-pressure screening. The soybean chloroplast genetic transformation method disclosed by the invention not only has important practical application values in soybean transgenic improvement and biologic reactor study, but also has an important reference significance in genetic transformation of other crop chloroplasts generated in a somatic embryogenesis mode.
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Description

Technical field

[0001] The invention belongs to the field of agricultural planting technology and relates to a genetic transformation technology. Background technique

[0002] Since the birth of the world’s first transgenic plant in 1983, nuclear genome transformation has always been the main research direction in the field of plant genetic engineering. However, with the deepening of research, the limitations of nuclear genome transformation technology have also attracted increasing attention. Due to the large nuclear genome of plant cells and the complicated genetic background, it is difficult to control the integration site and copy number of foreign genes in the nuclear genome. As a result, the expression efficiency of foreign genes is low and the expression is unstable, and "gene silencing" and " At the same time, exogenous transgenes easily spread with pollen to nearby relative species, leading to gene escape and endangering species diversity and ecological safety in nature...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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