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Rapid propagation method for suspension cell culture of Millettia speciosa Champ

A suspended cell and powerful technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as affecting yield and quality, increasing production costs, and small reproduction coefficients, so as to improve quality, yield, and production costs. Low, fast multiplication effect

Inactive Publication Date: 2017-02-15
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to solve the contradiction between supply and demand, people try to expand the drug source by artificial cultivation. Some successful research reports have been found so far, but there are still many problems restricting its large-scale production. It is very unstable, the survival rate of cutting seedlings is low, and it is very difficult for tissue culture seedlings to take root, etc.
Moreover, the growth cycle of cattle is long (generally 5 years before the production is formed), and it takes a long time to breed or raise seedlings by conventional methods. Due to the small reproduction coefficient and large seed consumption, the development speed is very slow and the production cost increases. The damage of plant diseases and insect pests seriously leads to degradation, which seriously affects the yield and quality

Method used

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  • Rapid propagation method for suspension cell culture of Millettia speciosa Champ
  • Rapid propagation method for suspension cell culture of Millettia speciosa Champ

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A rapid propagation method for bovine vigorous suspension cell culture, using the young cotyledons taken from the bovine vigorous pods 50 to 60 days after flowering as the culture material, through induction culture and subculture to obtain subculture callus, and then through Suspension cell culture Obtain stable bovine vigorous suspension cell culture fluid.

[0029] Wherein, the method for obtaining the culture material is as follows: select the pods of Niu Dali pods 50 to 60 days after flowering, use cotton to dip in the volume concentration of 75% ethanol to wipe the pods on the ultra-clean workbench, and carry out surface sterilization on the pods. Then carefully peel off the fruit pods, use sterilized tweezers to pick up the immature seeds inside the fruit pods, place them on a sterile plate or sterile filter paper, then use a sterile scalpel to cut the seed coat, and use sterilized tweezers to pick up The cotyledon is the culture material.

[0030] Wherein, the ...

Embodiment 2

[0039] A rapid propagation method for bovine vigorous suspension cell culture, using the young cotyledons taken from the bovine vigorous pods 50 to 60 days after flowering as the culture material, through induction culture and subculture to obtain subculture callus, and then through Suspension cell culture Obtain stable bovine vigorous suspension cell culture fluid.

[0040] Wherein, the method for obtaining the culture material is as follows: select the pods of Niu Dali pods 50 to 60 days after flowering, use cotton to dip in the volume concentration of 75% ethanol to wipe the pods on the ultra-clean workbench, and carry out surface sterilization on the pods. Then carefully peel off the fruit pods, use sterilized tweezers to pick up the immature seeds inside the fruit pods, place them on a sterile plate or sterile filter paper, then use a sterile scalpel to cut the seed coat, and use sterilized tweezers to pick up The cotyledon is the culture material.

[0041] Wherein, the ...

Embodiment 3

[0049] A rapid propagation method for bovine vigorous suspension cell culture, using the young cotyledons taken from the bovine vigorous pods 50 to 60 days after flowering as the culture material, through induction culture and subculture to obtain subculture callus, and then through Suspension cell culture Obtain stable bovine vigorous suspension cell culture fluid.

[0050] Wherein, the method for obtaining the culture material is as follows: select the pods of Niu Dali pods 50 to 60 days after flowering, use cotton to dip in the volume concentration of 75% ethanol to wipe the pods on the ultra-clean workbench, and carry out surface sterilization on the pods. Then carefully peel off the fruit pods, use sterilized tweezers to pick up the immature seeds inside the fruit pods, place them on a sterile plate or sterile filter paper, then use a sterile scalpel to cut the seed coat, and use sterilized tweezers to pick up The cotyledon is the culture material.

[0051] Wherein, the ...

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Abstract

The invention discloses a rapid propagation method for suspension cell culture of Millettia speciosa Champ. According to the rapid propagation method, young and tender cotyledons taken from Millettia speciosa Champ pods 50-60 days after flowering are utilized as culture materials, subcultured callus tissue is obtained through inducing culture and subculture, and a stable Millettia speciosa Champ suspension cell culture fluid is obtained through the suspension cell culture. With the adoption of the method, stable Millettia speciosa Champ suspension cells with good quality and high propagation yield can be obtained, and a novel way and method are provided for development and utilization of effective components of the Millettia speciosa Champ and creation of new germplasm resources from the Millettia speciosa Champ with biotechnology methods such as somatic hybridization, genetic transformation, mutant induction and the like.

Description

technical field [0001] The invention relates to the technical field of bovine vigorous suspension cell culture. More specifically, the present invention relates to a rapid propagation method of bovine vigorous suspension cell culture. Background technique [0002] Niu Dali is a plant of the genus Millettia speciosa Champ. in the leguminous family, also known as Dali potato and mountain lotus root. ) local medicinal materials. The root of Niu Dali is used as medicine, which is sweet and flat. Return lung, kidney channel. It has the effect of tonifying deficiency and nourishing the lungs, strengthening tendons and activating collaterals. It has been clinically proven to have good curative effect on chronic diseases such as lumbar muscle strain, rheumatoid arthritis, lung deficiency cough, tuberculosis, chronic bronchitis, and chronic hepatitis. The folks of the Zhuang people are often used for the treatment of lumbago and leg pain, fever (rheumatic bone pain), tuberculosis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 姚绍嫦白隆华蓝祖栽翟勇进黄浩韦荣昌
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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