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Plant in-vivo somatic cell hybridization method

A somatic and plant technology, applied in the field of plant improvement, can solve unseen problems and achieve the effect of wide application prospects

Active Publication Date: 2022-06-07
深圳前海觉民科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yet, before the present invention, also do not see the report of plant and microorganism, plant and animal somatic cell hybridization success

Method used

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  • Plant in-vivo somatic cell hybridization method
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  • Plant in-vivo somatic cell hybridization method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Selection and breeding of oyster maize in somatic hemisphere hybridization 1 breeding variety

[0035] The inbred line of the test corn is 4131 (selected from the hybrid maize Xianyu 335 isolation generation), and the parent body cells are from Zhanjiang oysters and purchased from Zhanjiang Seafood Market. First according to the volume of the desired somatic hybridizer, respectively, 200mg / L pectinase, 500mg / L cellulase, 150mg / L gibberellin, 155mg / L auxin, 2300μl / L enzyme lysate and 2500mg / L complex amino acids to the measuring cup, add distilled water to stabilize the volume and stir well, refrigerate and spare (the complex amino acids purchased from Shanghai Haring Biotechnology Co., Ltd. compound amino acid powder, the enzyme solution is a complete glycosyl decomposition of animal glycoproteinase digestion of enzymatic solution, The enzymatic solution used in the present embodiment is specifically purchased from the glycoprotein complete glycosyl decomposition...

Embodiment 2

[0057] Example 2 in somatic hemisphere hybridization 1 breeding variety - selection and breeding of birch mushroom rice

[0058]The rice cultivar (mother) for test is Super Rice Y Liangyou 900, provided by Hunan Hybrid Rice Research Center. The functional ingredient donor for the test is birch mushroom (Chaga brown foraminifera), quoted from the russian city of Blagoveshchensk. Birch mushrooms are propagated with improved Yao Jing birch mushrooms (Yao Jing. Test of domestication conditions of the medicinal fungus Chaga[J].Edible and medicinal fungi, 2018.26(2):99-100) activated and expanded, and then added 150 mg / L pectinase, 230 mg / L cellulase, 110 mg / L gibberellin, 235 mg / L auxin, 2000 μl / L enzyme solution and 3100 mg / L complex amino acids to a measuring cup according to the volume of the desired somatic hybrid agent, add distilled water to sizing and stir well, and refrigerate and set aside ( The complex amino acid is purchased from the compound amino acid powder of Shanghai Ha...

Embodiment 3

[0090] Example 3 In somatic hemisphere hybridization 1 breeding variety functional fern new variety - birch mushroom kidney fern breeding

[0091] First cut off the upper part of the neck egg organ of the excellent fern breed kidney fern gamete, remove the part of the sperm-generating organ, cultivate in the new incubator until the neck egg organ is mature, and then prepare the birch mushroom semen according to the preparation method of the birch mushroom semen in Example 2, drop 1 to several drops of enzymatic solution with a concentration of 2700 μl / L on the neck egg organ, so that the neck egg organ is wet with a brush to brush the prepared sperm, and finally isolate it with a sheepskin paper bag tightly bagged. This is continuously inseminated for 6-12 days, once a day, until successful fertilization and the acquisition of birch kidney fern sporophytes.

[0092] After the birch kidney fern sporozoites were cultured into seedlings, the chromosomes were doubled with 0.3% colchic...

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Abstract

The invention relates to a plant in-vivo somatic cell hybridization method which comprises the following steps: selecting a female organ of a good variety of a plant type expected to be obtained and a somatic cell cluster of a reproduction unit as a hybridization female parent receptor, performing treatment before insemination to remove reproduction isolation, so that the hybridization female parent receptor is easy to fertilize; individuals, organs, tissues, cells, chromosomes or chromosome segments, DNA or DNA segments and genes of animals or plants, microorganisms and other organisms with the characters of the expected variety are selected as male parent donors, a somatic cell hybridization agent is added and stirred evenly, a sperm is obtained, the sperm is used for insemination of a receptor under mediation of the somatic cell hybridization agent, and the variety is obtained. And embryo rescue and seedling culture are carried out after the fertilization embryo is taken back. The bottleneck of distant hybridization is broken through, high-yield and high-quality varieties can be cultivated, and the method has wide application prospects.

Description

Technical field [0001] The present invention belongs to the field of plant improvement. Specifically, it involves a method of hybridizing plants in somatic cells. Background [0002] Although as early as 1972, plant ex vivo cell hybridization has been successful, but because somatic cell hybridization is carried out indoors, the number of receptors is greatly limited, and more importantly, due to the departure of the mother, the hybridization positive rate is too low, the population for offspring to choose excellent individuals is too small, and the probability of breeding excellent varieties is too low, so there is rarely a large area used in the production of somatic cell hybrid breeding varieties. Somatic cell hybridization can make up for most of the above deficiencies. However, prior to the present invention, there have been no reports of successful hybridization in somatic cells. Therefore, sexual hybridization is still the most commonly used method of plant improvement. ...

Claims

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Application Information

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IPC IPC(8): A01H1/02C12N5/04C12N15/05
CPCA01H1/02C12N5/04C12N5/0025C12N15/02
Inventor 何觉民曾松亭何仪唐林华周建伟何蓉
Owner 深圳前海觉民科技有限公司
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