Seaweed breeding method based on induction polyploid gametocyte

A gametophyte and polyploid technology, which is applied in the field of kelp breeding based on induced polyploid gametophytes to achieve the effects of improving economic traits, enhancing physiological activities and increasing gene content

Active Publication Date: 2010-12-15
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the current kelp breeding work generally adopts hybrid breeding technology, the breeding materials are all haploid kelp gametophytes or kelp gametophyte clones, and the hybrid kelp obtained is still diploid

Method used

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  • Seaweed breeding method based on induction polyploid gametocyte

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Embodiment Construction

[0012] The present invention is further illustrated by specific examples below.

[0013] The experimental material of this embodiment is kelp female gametophyte cells. The kelp with sporangia is cleaned with disinfected seawater, dried in the shade for 3-4 hours, and then placed in low-temperature disinfected seawater to release the spores. Filter the spore water to collect the spores on the glass slide. The collected spores are cultured at a temperature of 15-20℃, with a light intensity of about 1500-2000lx, and a light time of 10h / day. The culture medium is filtered and disinfected seawater, plus nutrient inorganic nitrogen (N content is 4ppm) and inorganic phosphorus (P content) Is 0.4ppm). After the spores are attached to the glass slide, after 5-7 days of culture, the male and female gametophytes can be clearly distinguished. Female gametophytes are single-celled, with larger cells and darker pigments; male gametophytes are multicellular individuals with smaller cells and ...

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Abstract

The invention discloses a seaweed breeding method based on an induction polyploid gametocyte, which is characterized by comprising the following steps: firstly processing haploid seaweed gametophyte cells by utilizing mitosis hinder agent to lead the chromosome in the cells to be doubled, so as to induce the haploid seaweed gametophyte cells into polyploid seaweed gametophyte cells, and then breeding polyploid seaweed thalluses by utilizing a seaweed gametophyte cell hybridization technique. The method of the invention has the advantages that the seaweed gene content can be improved through polyploid breeding; and the seaweed physiological activity can be strengthened by the increased quantitative character gene, so as to improve economic character thereof. The chromosome doubling method of the invention can be applied for breeding homology polyploids and allopolyploids.

Description

Technical field [0001] The invention relates to an algae breeding method, in particular to a kelp breeding method based on inducing polyploid gametophytes. Background technique [0002] There is no natural kelp polyploid in nature, and the kelp varieties cultivated by the current kelp breeding work are all diploid kelp. Although hybrid breeding techniques are commonly used in kelp breeding, the breeding materials are haploid kelp gametophytes or kelp gametophyte clones, and the hybrid kelp obtained is still diploid. The expression of economic traits of kelp mainly depends on its own genetic genes for quantitative traits. In diploid kelp, the genetic content of quantitative traits is limited, and the content of different species varies little. Therefore, on this basis, there is room for further improvement of economic traits of kelp Is limited. Summary of the invention [0003] In view of the above-mentioned problems, the purpose of the present invention is to provide a kelp bree...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H1/02
CPCA01H1/08
Inventor 刘涛
Owner OCEAN UNIV OF CHINA
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