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Seaweed breeding method based on induction polyploid gametocyte

A gametophyte and polyploid technology, which is applied in the field of kelp breeding based on induced polyploid gametophytes to achieve the effects of improving economic traits, enhancing physiological activities and increasing gene content

Active Publication Date: 2011-12-14
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the current kelp breeding work generally adopts hybrid breeding technology, the breeding materials are all haploid kelp gametophytes or kelp gametophyte clones, and the hybrid kelp obtained is still diploid

Method used

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  • Seaweed breeding method based on induction polyploid gametocyte

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Embodiment Construction

[0012] The present invention is further illustrated below by specific examples.

[0013] The experimental material of this embodiment is Laminaria female gametophytic cells. The Laminaria species with sporangia are cleaned with sterile seawater, dried in the shade and stimulated for 3-4 hours, and then put into low-temperature sterile seawater to release spores. Filter the spore water to collect spores on glass slides. The collected spores are cultivated at a temperature of 15-20°C, with a light intensity of about 1500-2000lx, and a light time of 10h / day. is 0.4ppm). After the spores are attached to the slide, after 5-7 days of cultivation, the male and female gametophytes can be clearly distinguished. The female gametophytes are unicellular, with larger cells and darker pigments; the male gametophytes are multicellular individuals with smaller cells and lighter pigments. The male and female gametophytes are peeled off from the glass slide and placed in the culture medium. ...

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Abstract

The invention discloses a seaweed breeding method based on an induction polyploid gametocyte, which is characterized by comprising the following steps: firstly processing haploid seaweed gametophyte cells by utilizing mitosis hinder agent to lead the chromosome in the cells to be doubled, so as to induce the haploid seaweed gametophyte cells into polyploid seaweed gametophyte cells, and then breeding polyploid seaweed thalluses by utilizing a seaweed gametophyte cell hybridization technique. The method of the invention has the advantages that the seaweed gene content can be improved through polyploid breeding; and the seaweed physiological activity can be strengthened by the increased quantitative character gene, so as to improve economic character thereof. The chromosome doubling method of the invention can be applied for breeding homology polyploids and allopolyploids.

Description

technical field [0001] The invention relates to an algae breeding method, in particular to a kelp breeding method based on inducing polyploid gametophytes. Background technique [0002] There is no natural kelp polyploid in nature, and the kelp species cultivated by the current kelp breeding work are all diploid kelp. Although the current kelp breeding work generally adopts hybrid breeding technology, its breeding materials are all haploid kelp gametophytes or kelp gametophyte clones, and the obtained hybrid kelp is still diploid. The expression of economic traits in kelp mainly depends on its own quantitative trait genetic genes. In diploid kelp, the genetic content of quantitative traits is limited, and the range of content variation among different species is not large. Therefore, there is room for further improvement of kelp economic traits on this basis. is limited. Contents of the invention [0003] In view of the above problems, the object of the present invention...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08A01H1/02
CPCA01H1/08
Inventor 刘涛
Owner OCEAN UNIV OF CHINA
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