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Method for preparing amaranthus protoplast and application of amaranthus protoplast

A technology of protoplasts and amaranth, applied in the field of preparation of amaranth protoplasts, can solve problems such as immaturity and transformation difficulties of amaranth, and achieve the effect of promoting development

Pending Publication Date: 2022-01-07
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the genetic transformation system of amaranth is still immature, and transformation is still relatively difficult.

Method used

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  • Method for preparing amaranthus protoplast and application of amaranthus protoplast
  • Method for preparing amaranthus protoplast and application of amaranthus protoplast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Cultivation of sterile vaccines:

[0039]The seeds of amaranth variety (Jingzhu No. 3) were taken, disinfected with 10% Kao disinfectant for 15 minutes, and washed 5 times with sterile water. The MS medium was sterilized by high pressure steam and dispensed into culture bottles. Inoculate the sterilized seeds evenly on the MS medium, seal the bottle mouth, and place it in a sterile tissue culture room for cultivation. The culture conditions are: 25°C, 16 hours of light and 8 hours of darkness every day. Cultivate until the seedlings grow true leaves (it takes about four weeks, and the seedlings are about 10 cm long), to obtain aseptic seedlings of amaranth.

[0040] 2, prepare amaranth protoplast:

[0041] 1) Take 1g of the young true leaves of aseptic seedlings of amaranth, and cut them into no more than 1mm with a sharp double-sided knife 2 fragments. Immediately soak the fragments in a 0.4M mannitol solution to prevent tissue dehydration.

[0042] 2) Transfe...

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Abstract

The invention provides a method for preparing amaranthus protoplast. The method is characterized in that tender true leaves of amaranthus sterile seedlings are used as materials, filter residues are extruded during filtration after enzymolysis, a large number of amaranthus protoplast with normal form, few impurities and no damage is prepared, the experimental requirements of subsequent conversion, subcellular localization and the like can be met, and important technical support can be provided for fundamental researches and application researches such as dual-fluorescence complementation, somatic cell hybridization and genetic transformation, so that the development of genetic breeding and fundamental researches of the edible amaranthus is promoted.

Description

technical field [0001] The invention specifically relates to a method for preparing amaranth protoplasts and its application. Background technique [0002] Amaranth (Amaranthus spp.) is a general term for various seedlings and shoots of the genus Amaranth in the family Amaranthaceae that can be used as vegetables, and there are many ways to eat it. The cultivated amaranth is mainly amaranth (Amarnanthus tricolor). Because amaranth contains betalain, many varieties have unique red and green colors in their leaves. In recent years, it has become more and more popular in the market, and the market demand has gradually increased. Therefore, the research on amaranth and its relatives has more and more important biological significance and industrial value. [0003] A typical plant cell has a cell wall. Plant protoplasts refer to the naked cells that are only wrapped by the plasma membrane and can carry out certain life activities by removing the plant cell wall by artificial m...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12N5/14C12N15/82G01N21/64
CPCC12N5/04C12N15/8206C12N5/14G01N21/6486
Inventor 李大勇刘雪张彬
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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