The ssr primer set developed based on the transcriptome sequencing information of A. tomentosa and its application in germplasm identification

Abstract

The invention discloses an SSR (Simple Sequence repeats) primer group based on Fraxinus Velutina Torr. transcriptome sequencing information development and an application of the primer group in germplasm identification, belonging to the technical field of molecular biology. The SSR primer group comprises 42 pairs of primers, wherein the nucleotide sequences of the first pair to the forty-second pair of primers are shown in SEQ ID No.1 to SEQ ID No.84. Experiments verify that the SSR primer group disclosed by the invention has the advantages of rich polymorphism, good repeatability, clear electrophoretic band and the like. The SSR primer group can be effectively used for researches on germplasm identification, DNA fingerprint establishment and the like. A germplasm identification method is sensitive and reliable, can be used for rapidly and accurately realizing and finishing distinguishment and identification of Fraxinus chinensis germplasm, and has important significances in identification and intellectual property protection of Fraxinus chinensis germplasm resources and genetic breeding of genetic breeding molecules.

Description

technical field [0001] The invention relates to a set of SSR primers developed based on the sequence information of the transcriptome of A. tomentosa and its application in germplasm identification, belonging to the field of biotechnology. Background technique [0002] Fraxinus Velutina Torr. is a deciduous tree of the genus Fraxinus Linn. in the family Leaceae. It is native to the United States and was introduced to Jinan, Shandong Province in 1911 (Meng Zhaohe et al., 2001). It is fast-growing, beautiful, and stress-resistant. It has become the main landscaping tree species in the coastal cities of North China and East China and excellent saline-alkali land afforestation tree species (Shi Mingzhi, 1996; Wang Qin et al., 2000; Ni Guoxiang et al., 1995; Wang Youping, 2007), and it is also the only large tree species in saline-alkali land. Due to the lack of a large number of polymorphic molecular markers, the research on its genetics and genomics lags behind the tree species...

AI Technical Summary

Problems solved by technology

Compared with woody plants such as poplar and tea tree, white wax has only European white wax SSR markers (only 11 pairs), and Wang Jianbing et al. have developed 3 pairs of polymorphic white wax SSR markers through the EST database (2014 ), the amount is very small, far from meeting the needs of white wax research, so developing a large number of SSR markers is still one of the important tasks of white wax research

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