44results about How to "Good material basis" patented technology

The invention provides a compound Chinese medicinal oral preparation for treating alopecia areata and alopecia and its preparing method, wherein the preparation comprises astragalus root, white atractylodes rhizome, arborviate seed, prepared fleece flower root, root of herbaceous peony, cherokee rose, Chinese angelica root, Ligusticum wallichii, eclipta, ligustrum japonicum, psoralea fruit, mulberry, root of red rooted saliva, black sesame seeds, dodder seed.

The invention discloses a transverse-furrow and sequential-ridge mellow soil anti-corrosion farming method of a purple soil slope farmland and particularly relates to the field of a soil erosion management technique of the purple soil slope farmland. In the method disclosed by the invention, a transverse furrow is excavated along the contour line on the purple soil slope farmland at the slope length interval which is less than the rill erosion generation critical length of the modified slope surface; the cross section of the transverse furrow is in an arc shape; the formed transverse furrow divides the whole slope farmland plot into multiple small farming units in the transverse slope direction; the two ends of the transverse furrow are connected with the original drainage furrows at the two ends of the slope farmland plot; and the integral farming terrain conditions with alternate furrows and ridges are formed by adopting a sequential-slope ridging farming mode, i.e. alternately farming and ridging in the sequential slop direction and reverse slop direction. The method disclosed by the invention can reduce the water and soil loss and prevent and control the soil erosion of the slope farmland, is beneficial to the enhancement of the water content in the soil of the farmland plot in the descending direction of the transverse furrow, improves the drought resistance of crops, maintains the agricultural productivity of the slope farmland and realizes the sustainable utilization of slope farmland water and soil resources.

The invention discloses a method for preparing nattokinase by a solid state fermentation method. The method comprises unit operations such as soybean pretreatment, culture medium preparation, seed preparation, inoculation, temperature-variable fermentation, drying and smashing. The method is characterized in that the techniques such as raw material pretreatment, culture medium and seed preparation, inoculation, fermentation, drying and smashing are integrated; the whole process is performed in clean or bacteria-free environment; poor phenomena such as flowing, overflowing, dripping and leaking cannot be generated; the GMP (good manufacturing practice) requirements can be easily met. Compared with a traditional method, the method has the following characteristics that the ingenious raw material pretreatment and multicomponent culture medium composition design provides a good material base for the growth of nattokinase producing bacteria; a surfactant is used, so that the generation and the secretion of the nattokinase are obviously improved; through the application of a temperature-variable fermentation method and process, the nattokinase yield is improved by 10 to 45 percent; the method is scientific; the process is reasonable and advanced; the environment protection is facilitated. The method can be widely applied to development and production of nattokinase and other relative products.

The invention discloses a method for preparing nattokinase by a liquid fermentation method. The method comprises strain activation, culture medium preparation, seed preparation, inoculation, temperature change fermentation and post-treatment. The method combines strain activation, culture medium preparation, seed preparation, inoculation, fermentation and post-treatment. The whole process is carried out in a clean or sterile environment. The method does not produce escape, overflow, dropping and leakage and satisfies GMP requirements. Compared with the traditional method, the method provided by the invention utilizes an ingenious multi-component medium design and provides a good material basis for growth of nattokinase production bacteria. Through use of a surfactant, nattokinase production and secretion are significantly improved. Through use of the temperature change fermentation method and process, a nattokinase yield is improved by 6-38%. The method has reasonable and advanced processes, is suitable for expanding reproduction, significantly improves a yield and product quality, is environmentally friendly and can be widely used for research and development of nattokinase and other related products.

The invention provides a process for preparing a feed by adopting lactobacillus acidophilus pomace. The process comprises two steps of a strain expanding propagation process and a pomace fermentation step, wherein the strain expanding propagation process is as follows: carrying out activation, cultivation, liquid cultivation and inoculating expanding cultivation onto lactobacillus acidophilus freeze-dried powder in sequence to obtain the lactobacillus acidophilus strain. The pomace fermentation step is as follows: after removing impurities for the fresh pomace, uniformly mixing with the lactobacillus acidophilus strain obtained by expanding propagation, sealing and fermenting for 7-10 days under a temperature condition of 15DEG C-30 DEG C to obtain the wet pomace fermented feed. After fermenting apple pulp by adopting the lactobacillus acidophilus, substances such as various alcohols, aldehyde, acetone, acids, esters, and the like with fragrance are produced, so that palatability of the pomace fermented feed is strengthened, and therefore, the lactobacillus acidophilus pomace fermented feed has effect of improving animal feed intake; and moreover, nutrient characteristics of the apple pulp are effectively improved, so that good material basis is provided for dairy cow yield increase. As a beneficial bacterium, the lactobacillus acidophilus improves anti-stress ability of animals and resistance to diseases.

The invention relates to 5-(1H-indolyl-3-methylene)-1,3-thiazolidinyl-4-one derivatives, and a synthesis method and application thereof. By using ethanol and / or water as a solvent, substituted 2-substituted-imino-1,3-thiazolidinyl-4-one and 1H-indolyl-3-formaldehyde are subjected to reflux reaction under the catalytic condition of piperidine through intermolecular dehydration condensation reaction to form methylene linking group, thereby obtaining the 5-(1H-indolyl-3-methylene)-1,3-thiazolidinyl-4-one derivatives. The intermediate 2-substituted-iminothiazolidinyl-4-one is prepared by carrying out cyclization reaction on various monosubstituted ethyl thiocarbamide chloroacetates or chloroacetic acids in a low-boiling solvent under reflux conditions, and the intermediate 2-substituted-imino-3-substituted-1,3-thiazolidinyl-4-one is prepared by carrying out a green environment-friendly synthesis technique on various disubstituted symmetric thiocarbamides and chloroacetic acids. The bioactivity preliminary screening experiment result of all the target compounds on the enzyme molecular level indicates that the target products have certain inhibition activity on PTP1B and CDC25B to different degrees.

A traditional Chinese medicine extract for preventing hypertrophic Sclerotinia sclerotiorum adopts the methods of heating alcohol, continuous refluxing for extracting, etc. The extract is obtained from the cortex fraxini through a series of process flows. The antimicrobials prepared by the extract can effectively prevent the hypertrophic Sclerotinia sclerotiorum. The efficacy test indicates that the control rate to hypertrophic Sclerotinia sclerotiorum is above 95% and the total control rate can obtain more than 85%. The preparation belongs to the pure traditional Chinese medicine preparation and has the advantages of prevention of application of chemical pesticide, reduced environment pollution, abroad source of raw material for production and low cost for manufacturing.

The invention discloses long-shelf-life fermented soybean. The long-shelf-life fermented soybean is prepared from black soybeans, salt, peppers, Fructus Litseae, Fructus Zanthoxyli, borneol, Radix Scutellariae, Cortex Pseudolaricis and purified water. The long-shelf-life fermented soybean is free of colorants, additives and the like and low in harmful substance content and belongs to purely natural fermented green safe food; a finished production of the long-shelf-life fermented soybean is black brown, oily, glossy, tasty, tender, free of astringent taste, refreshing and rich in flavor and imperishable in aroma after long-time storage; by addition of the Chinese herbal medicines, the shelf life of the fermented soybean is remarkably prolonged while the fermented soybean has dietary therapy efficacies of Qi regulating, stasis removing, liver protection, diuresis promoting, antibacterial and anticancer efficacies and the like, and high edible and healthcare values are achieved. In addition, the preparation method is scientific and reasonable in process, preparation time is shortened by 60-80% as compared with that of a traditional preparation method, and the preparation method is suitable for large-scale factory production and high in promotion value.

The invention discloses an efficient extraction method of selenium-enriched mushroom powder selenium polysaccharide, which comprises the following steps: mixing selenium-rich mushroom powder with ethanol, refluxing, carrying out suction filtration, and carrying out vacuum drying to obtain degreased mushroom powder; mixing the dried degreased mushroom powder with distilled water, adjusting the pH value, adding cellulase, carrying out ultrasonic enzymolysis, heating for enzyme deactivation, carrying out high-temperature digestion, and centrifuging to obtain an extract liquid; carrying out rotaryevaporation concentration, alcohol precipitation and centrifugation on the extract liquid to obtain precipitate, and carrying out vacuum drying to obtain crude selenium polysaccharide; and redissolving the crude selenium polysaccharide with distilled water, adding neutral protease for enzymolysis, centrifuging, removing protein from the supernatant with a Sevage reagent, centrifuging, dialyzing,and freeze-drying to obtain the mushroom powder selenium polysaccharide. According to the method, the selenium polysaccharide is extracted by adopting an ultrasonic synergistic cellulase method assisted by high-temperature digestion, so that the extraction rate of the selenium polysaccharide is greatly improved; protease-Sevage reagent combined protein removal is adopted, the protein removal effect is good, and selenium polysaccharide loss is small. The extraction method is simple and feasible, the extraction rate of the selenium polysaccharide is high, impurities are few, and the method has good application value and market potential.

The invention relates to a grafting method, in particular to a walnut top grating method. Scions are collected in January during the dormancy period and stored in freshness-retaining air-conditioned cold store with the temperature of 3 DEG C, bark grafting or bark and whip grafting are carried out during the last ten days of the March and the middle ten days of the April, stock sprouts are removed in time after grafting, and pinching is carried out when young sprouts grow to be 60-80cm. The top grafting survival rate can exceed 85% all the year around. According to the walnut top grafting method, the scions are refrigerated. Thus, the contents of soluble protein, the total organic carbon and potassium in the scions can be improved, the contents of tannin, plant polyphenol, soluble sugar and sodium in the scions can be reduced, the contents of water, total nitrogen and magnesium in the scions are not obviously affected, and good material foundation for improving the grafting survival rate is improved. The grafting is carried out during the last ten days of the March and the middle ten days of the April, the water content and the bleeding exudation of the stocks are the lowest, the temperature is favorable for healing wound of cell division, the early spring with large temperature fluctuation is avoided, and the favorable environmental conditions for improving the grafting survival rate is provided. The appropriate top grafting method of bark grafting or bark and whip grafting is chosen. The bark grafting method or the bark and whip grafting method is simple and easy to learn, so that the technical guarantee for improving the grafting survival rate is provided.

The invention discloses a tail gas recycling device for transforming a hot air grain dryer. A tail gas recycling system is formed by a tail gas recycling cover, a dust collecting hopper, a tail gas recycling pipeline, an axial flow fan and a purified tail gas cover. A tail gas purifying system is formed by a gravity dust settling chamber, a scraper purifier, a dust removing fan and an air supply tail gas pipeline. A cold air preheating system is formed by converting the third set of heat exchangers of an original hot air grain dryer into an air preheater. A flue gas recycling system is formedby a first flue gas pipeline, a flue gas adjusting valve, a second flue gas pipeline, a flue gas check valve, a cold air pipeline, a cold air adjusting valve and a mixed flue gas pipeline. The tail gas recycling device has the advantages of reducing the drying cost, ensuring the grain drying yield, improving the drying efficiency, reducing the pollution and the like.

The invention relates to a method for the facility TanTsing of tea leaves, which is realized by specific equipment and a matching process thereof in a heating chamber. By the control of the conditions of an air conditioner, a dehumidifier, an ultrasonic atomizer and a fan, the precise regulation and control of the humiture condition of the TanTsing chamber and the effective regulation of environmental gas, the purpose of the proper TanTsing of the fresh leaves is achieved. The invention can realize the precise regulation and control of the humiture, the gas circulation and the like of a TanTsing space, further regulate and control the approach and the degree of a physical and chemical reaction of the fresh leaves under a specific micro-area environmental condition and realize the regulation of gas in a micro-area environment, thereby inducing the beneficial transformation of the contained components and providing a better material basis for a subsequent processing procedure.

The invention discloses a bio-organic fertilizer which is prepared from the following components in parts by weight: 30-40 parts of cow dung, 20-30 parts of pig manure, 20-40 parts of straws, 10-20 parts of weeds, 10-15 parts of peanut shell, 10-20 parts of chicken feather, 5-10 parts of algae mud, 5-10 parts of rapeseed meal, 3-6 parts of quick lime, 10-15 parts of mint leaf, 1-3 parts of EM strain, 40-60 parts of water, 2-5 parts of sugar and 8-12 parts of lavender. The bio-organic fertilizer disclosed by the invention is capable of increasing beneficial microorganisms in soil and reducing pathogenic bacteria, so as to improve the fertility of bio-organic fertilizer and reduce the preparation cost of bio-organic fertilizer; the bio-organic fertilizer is capable of improving soil while expelling injurious insects in soil, so as to boost the growth of crops; the bio-organic fertilizer is capable of improving pH value, permeability and water retention capacity of soil, so as to effectively improve physicochemical biological characters of soil, establish an excellent material basis for promoting growth and yield of crops and bring a better use prospect.

The invention relates to the field of daily necessities and particularly relates to a mattress. The mattress comprises a first 3D fabric layer, an inflatable elastic cushion layer, a second 3D fabric layer and a side wrapping layer wrapping a side face of the mattress, wherein the first 3D fabric layer, the inflatable elastic cushion layer and the second 3D fabric layer are arranged in a stacked mode, the side wrapping layer wraps a side face of the mattress, and the inflatable elastic cushion layer is clamped between the first 3D fabric layer and the second 3D fabric layer and is of an extractable structure; the side coating layer comprises an upper side coating layer connected with the first 3D fabric layer and a lower side coating layer connected with the second 3D fabric layer, the upper side coating layer is connected with the lower side coating layer through a connecting unit, the connecting unit is arranged on the side face of the mattress, thickness of the first 3D fabric layer is 5-8 cm, thickness of the second 3D fabric layer is 0.8-1cm, and the upper side coating layer and the first 3D fabric layer as well as the lower side coating layer and the second 3D fabric layer are respectively provided with an edge covering structure. The mattress is used for solving a problem that the comfort, air permeability and logistics cost of the mattress cannot be considered at the same time.

The invention provides a method for judging vegetation recovery of plots as wetlands returned from grain plots in plain areas, a vegetation recovery method and a vegetation recovery effect evaluatingsystem, relates to the method for judging vegetation recovery of plots as wetlands returned from grain plots in plain areas, the vegetation recovery method and the vegetation recovery effect evaluating method, and aims to solve the technical problems that in the prior art, a method for judging recovery of plots as wetlands returned from grain plots in plain areas and a method for evaluating effects do not exist. The method for judging vegetation recovery of plots as wetlands returned from grain plots in plain areas and the evaluating method comprise the following steps of I, determining the plots to be returned from grain plots, and collecting soil samples; II, executing a method for judging recovery; III, performing communication of water systems, establishing 40-60cm micro water level gradients of which the slope is smaller than 5 degrees, and performing wetland recovery; and IV, evaluating the recovery effects. When the methods disclosed by the invention are used, after the wetlandsare recovered at the plots as wetlands returned from grain plots, fixed quadrats are established, vegetation investigation is continuously performed, compared with a contrast wetland, the recovered wetland has the progress that the biological diversity is recovered to 72%, and the biomass is recovered to 90%, and the wetland recovery effects of the plots as wetlands returned from grain plots areideal. The invention belongs to the field of returning the grain plots to wetlands.

The invention discloses a recombinant MSG1 protein monoclonal antibody and a blocked ELISA (Enzyme-linked Immuno Sorbent Assay) method for detecting a haemophilus parasuis mycoplasma specific antibody. A hybridoma cell line 1A7 of an excretion haemophilus parasuis mycoplasma recombinant MSG1 protein monoclonal antibody is preserved with preservation number of CGMCC NO. 4860 and the preservation date of May 13th, 2011. The monoclonal antibody of the haemophilus parasuis mycoplasma recombinant MSG1 protein is excreted by the hybridoma cell line 1A7 with preservation number of CGMCC NO. 4860. The monoclonal antibody as well as the haemophilus parasuis mycoplasma MSG1 recombinant protein and an etiology protein are provided with good reaction specificities. According to the invention, the monoclonal antibody is applied to the blocked ELISA (Enzyme-linked Immuno Sorbent Assay) method for detecting the haemophilus parasuis mycoplasma specific antibody; and the detection condition is optimized through a large quantity of experiments so that the method has the advantages of good repeatability, sensitivity and specificity.

The invention discloses a method which is used for naturally simulating a community and based on construction of a scaffold, and relates to the technical field of ecological restoration. The method includes the following steps of: investigating a landform of a pit, performing data mapping, cleaning the pit, setting up fixing holes, performing insertion and connection on fixing columns, overlappingcrossbars, laying a screen mesh, performing filling with planting soil, planting plants and performing regular maintenance. According to the topography and landform of the pit, construction of the scaffold can be performed in the pit, good external conditions for plant growth can be provided by laying the screen net and transplanting the planting soil, so that the process of community successionis simulated, green returning of the pit is achieved, and the ecological environment of the pit is changed.

The invention discloses pretreated carbonaceous gold concentrate, a preparation method thereof and a gold extraction method. The preparation method and gold extraction method comprise the steps that carbonaceous gold concentrate powder is pressed into carbonaceous gold concentrate blocks; the carbonaceous gold concentrate blocks are subjected to roasting in vacuum, organic carbon in the carbonaceous gold concentrate blocks is converted into graphite carbon, and cooling is performed after roasting is completed; the pretreated carbonaceous gold concentrate is ground into pretreated carbonaceousgold concentrate powder; and cyanidation leaching is performed on the pretreated carbonaceous gold concentrate powder. According to the pretreated carbonaceous gold concentrate, the preparation methodthereof and the gold extraction method provided by the invention, a vacuum system is combined with roasting, and the carbonaceous gold concentrate can be effectively pretreated when the vacuum pressure is controlled not to be greater than 20 Pa, the temperature is controlled to be 1000-1200 DEG C and the roasting time is controlled to be 50-90 min; through reaction, the organic carbon with a goldintercepting effect can be converted into the graphite carbon without a gold intercepting effect, the gold intercepting capability of the carbonaceous gold concentrate is weakened, and influences ofthe concentrate on cyanidation gold extraction are eliminated; and gold wrapped with sulphide ore can be further dissociated, a good material basis is provided for cyanidation gold extraction, the gold leaching efficiency is improved, and the leaching duration is shortened.

The present invention relates to a coal base nutrient agent for improving soil, belonging to the field of organic and inorganic composite fertilizer technology. Its composition includes (by wt%) 30%-70% of danty, 20%-60% of turf, 10%-30% of microbial fungus root and chemical reagent after which is added, its soil nutrient concentration includes N element 200-300 mg / kg, K element 200-300 mg / kg and p element 50-100 mg / kg. Said invention can raise capability of plant to absorb nutrients, can raise adverse resistance of plant and can obviously raise yield of crops.

The invention discloses a transverse-furrow and sequential-ridge mellow soil anti-corrosion farming method of a purple soil slope farmland and particularly relates to the field of a soil erosion management technique of the purple soil slope farmland. In the method disclosed by the invention, a transverse furrow is excavated along the contour line on the purple soil slope farmland at the slope length interval which is less than the rill erosion generation critical length of the modified slope surface; the cross section of the transverse furrow is in an arc shape; the formed transverse furrow divides the whole slope farmland plot into multiple small farming units in the transverse slope direction; the two ends of the transverse furrow are connected with the original drainage furrows at the two ends of the slope farmland plot; and the integral farming terrain conditions with alternate furrows and ridges are formed by adopting a sequential-slope ridging farming mode, i.e. alternately farming and ridging in the sequential slop direction and reverse slop direction. The method disclosed by the invention can reduce the water and soil loss and prevent and control the soil erosion of the slope farmland, is beneficial to the enhancement of the water content in the soil of the farmland plot in the descending direction of the transverse furrow, improves the drought resistance of crops, maintains the agricultural productivity of the slope farmland and realizes the sustainable utilization of slope farmland water and soil resources.

The invention belongs to the field of extraction of traditional Chinese medicines, and in particular relates to a method for simultaneous preparation of 7-hydroxy-4 '-methoxy flavan and pterostilbene. The method includes the steps of: 1) taking dracaena cochinchinensis grease-containing wood or ethanol extract thereof, grinding, adding petroleum ether to extract, filtering to obtain filter residue for standby use; 2) using chloroform for reflux extraction of the obtained filter residue, and recovering the chloroform to obtain chloroform extract; 3) using methanol to dissolve the obtained chloroform extract, performing column chromatography, eluting with methanol with every 250ml being one flow part, and merging 2-8 flow parts; 4) concentrating the merged 2-8 flow parts to dry, dissolving with methanol, using a reverse phase silica gel column for column chromatography, eluting with methanol with every 100ml being one flow part, and respectively merging 14-18 flow parts and 20-27 flow parts; and 5) concentrating the respectively merged 14-18 flow parts and 20-27 flow parts to dry, and respectively recrystallizing to obtain the two compounds of 7-hydroxy-4'-methoxy flavan and pterostilbene. The two compounds of 7-hydroxy-4 '-methoxy flavan and pterostilbene are firstly and simultaneously prepared from dracaena cochinchinensis by the method.

The invention relates to material refining equipment, in particular to a high shear dispersion emulsifying machine. The high shear dispersion emulsifying machine comprises a vertical main shaft connected with a motor at the upper end, wherein the lower end of the main shaft is connected with a rotor; a stator is fitted and mounted on the periphery of the rotor, and is fixed through a bracket arranged on the main shaft and between the lower ends; at least one circle of fixed tooth ring is formed on the stator; tooth grooves of each circle of fixed tooth ring are inclined grooves; the rotor is provided with at least one circle of movable tooth ring correspondingly; tooth grooves of each circle of movable tooth ring are straight grooves; each circle of movable tooth ring is sleeved on the inner side of corresponding fixed tooth ring at radial intervals. Under the high-speed driving of the motor, materials move with high speed in a narrow gap between the rotor and the stator to form turbulent flows, so that effects of dispersion, emulsification and crushing can be achieved, and excellent material foundation is provided for subsequent grinding procedure; at the same time, as the fitting manner of stator as the inclined groove and the rotor as the straight groove is adopted, the material shearing time is improved, and the better shearing effect is achieved.

The invention discloses a preparation method of fresh and fragrant green tea. The preparation method comprises the following steps of S1: spreading fresh tea leaves in a room for 16-24 hours at the temperature of 16-24 DEG C with the spreading thickness being 4-8cm, thereby obtaining fresh leaves with the water content being 50-65%; S2: removing green: taking the fresh leaves treated in the S1, removing green by using a green removing machine with the rotating speed being 28-32r / min, the temperature being 340-440 DEG C and green removing time being 3-8min, thereby obtaining green-removed leaves with the water content being 45-55%; S3: modeling: kneading and twisting for modeling the green-removed leaves by using a tea kneading machine; S4: drying: by using a tea-leaf drying machine, treating the modeled tea leaves with the temperature of an air inlet of the drying machine being 150-200 DEG C, the upper-leaf thickness being 3-6cm and the drying time being 8-15 min, thereby obtaining the formed leaves with the water content being 15-25%; S5: extracting fragrance: putting leaves treated by S4 into a box body type fragrance extracting machine for extracting fragrance, spreading the leaves till the thickness if 1-3cm, firstly drying for 10-20 min by adopting the temperature of 80-90 DEG C, then drying for 40-60 min by adopting the temperature of 60-70 DEG C, thereby obtaining the tea leaves with the water content being 4.5-6.5%. The preparation method disclosed by the invention is simple in process, short in production period, low in cost and suitable for industrial and large-scale production.

The invention relates to 5-(1H-indolyl-3-methylene)-1,3-thiazolidinyl-4-one derivatives, and a synthesis method and application thereof. By using ethanol and / or water as a solvent, substituted 2-substituted-imino-1,3-thiazolidinyl-4-one and 1H-indolyl-3-formaldehyde are subjected to reflux reaction under the catalytic condition of piperidine through intermolecular dehydration condensation reaction to form methylene linking group, thereby obtaining the 5-(1H-indolyl-3-methylene)-1,3-thiazolidinyl-4-one derivatives. The intermediate 2-substituted-iminothiazolidinyl-4-one is prepared by carrying out cyclization reaction on various monosubstituted ethyl thiocarbamide chloroacetates or chloroacetic acids in a low-boiling solvent under reflux conditions, and the intermediate 2-substituted-imino-3-substituted-1,3-thiazolidinyl-4-one is prepared by carrying out a green environment-friendly synthesis technique on various disubstituted symmetric thiocarbamides and chloroacetic acids. The bioactivity preliminary screening experiment result of all the target compounds on the enzyme molecular level indicates that the target products have certain inhibition activity on PTP1B and CDC25B to different degrees.

The invention discloses a synthetic method of an organic DSTMS crystal high-purity growth raw material. The method comprises the following steps of: 1, reacting mesitylene sulfonyl chloride and methanol serving as reactants and organic alkali piperidine serving as an acid-binding agent at room temperature to obtain 2, 4, 6-trimethylbenzenesulfonate; 2, dissolving 4-methylpyridine and the 2, 4, 6-trimethylbenzenesulfonate in absolute ethyl alcohol, and carrying out condensation reflux reaction to generate an intermediate product 4-methyl-N-methylpyridine- 2, 4, 6-trimethylbenzenesulfonate; and3, mixing p-dimethylaminobenzaldehyde with the intermediate product generated by the reaction by taking absolute ethyl alcohol as a solvent and organic alkali piperidine as a catalyst, putting the mixture into a stirrer, stirring, condensing, refluxing and reacting to finally obtain the DSTMS raw material. The DSTMS raw material synthesis process provided by the invention has the advantages of high yield, high purity, low cost and simple steps. Through adjustment of technological parameters, the DSTMS raw material with the yield of 75%-89% and the purity of 90-96% is finally obtained.

The invention relates to novel application of carbon nanotubes, in particular to application of carboxylated nitrogen-containing multi-walled carbon nanotubes to weight gain and immunity enhancement of a mouse. Studies in the invention find that MWCNTN-COOH with the using dose of 1.0mg / Kg can significantly increase the weight of the mouse, has a significant effect of enhancing the immune level ofthe mouse and can be used as a candidate material for a vaccine adjuvant, so that a good material basis is provided for development and utilization of a vaccine and gaps of the MWCNTN-COOH in biological metabolism and immunological research are filled.

The invention discloses an enzymolysis method of a sea bass byproduct and an application thereof in an emulsion product, belonging to the field of marine product deep processing. The enzymolysis method comprises the following steps: performing pretreatment to obtain a skim sea bass processing byproduct; regulating the material-water ratio and the temperature to 45-60 DEG C and pH to 8-10; adding alkaline protease for sufficient enzymolysis; without enzyme deactivation, further regulating the temperature to 40-55 DEG C and pH to 6-8; and adding flavorzyme for sufficient enzymolysis to obtain an enzymolysis product. In the invention, the alkaline protease and flavorzyme are adopted for step composite enzymolysis treatment of the sea bass leftovers, and the difference of two proteases in digestion site is fully utilized to realize mutual action and promotion. The obtained four enzymolysis products A, B, C and D have double advantages of rich nutrition and good emulsifying property and can partially replace sodium caseinate in a full nutriment; and the obtained formula meets the requirements of clinical emulsion products while more abundant polypeptide and amino acid can be provided.

The invention discloses a recombinant MSG1 protein monoclonal antibody and a blocked ELISA (Enzyme-linked Immuno Sorbent Assay) method for detecting a haemophilus parasuis mycoplasma specific antibody. A hybridoma cell line 1A7 of an excretion haemophilus parasuis mycoplasma recombinant MSG1 protein monoclonal antibody is preserved with preservation number of CGMCC NO. 4860 and the preservation date of May 13th, 2011. The monoclonal antibody of the haemophilus parasuis mycoplasma recombinant MSG1 protein is excreted by the hybridoma cell line 1A7 with preservation number of CGMCC NO. 4860. The monoclonal antibody as well as the haemophilus parasuis mycoplasma MSG1 recombinant protein and an etiology protein are provided with good reaction specificities. According to the invention, the monoclonal antibody is applied to the blocked ELISA (Enzyme-linked Immuno Sorbent Assay) method for detecting the haemophilus parasuis mycoplasma specific antibody; and the detection condition is optimized through a large quantity of experiments so that the method has the advantages of good repeatability, sensitivity and specificity.

The invention belongs to the field of extraction of traditional Chinese medicines, and in particular relates to a method for simultaneous preparation of 7-hydroxy-4 '-methoxy flavan and pterostilbene. The method includes the steps of: 1) taking dracaena cochinchinensis grease-containing wood or ethanol extract thereof, grinding, adding petroleum ether to extract, filtering to obtain filter residue for standby use; 2) using chloroform for reflux extraction of the obtained filter residue, and recovering the chloroform to obtain chloroform extract; 3) using methanol to dissolve the obtained chloroform extract, performing column chromatography, eluting with methanol with every 250ml being one flow part, and merging 2-8 flow parts; 4) concentrating the merged 2-8 flow parts to dry, dissolving with methanol, using a reverse phase silica gel column for column chromatography, eluting with methanol with every 100ml being one flow part, and respectively merging 14-18 flow parts and 20-27 flow parts; and 5) concentrating the respectively merged 14-18 flow parts and 20-27 flow parts to dry, and respectively recrystallizing to obtain the two compounds of 7-hydroxy-4'-methoxy flavan and pterostilbene. The two compounds of 7-hydroxy-4 '-methoxy flavan and pterostilbene are firstly and simultaneously prepared from dracaena cochinchinensis by the method.

A multipurpose nutrient used as both food and cosmetic for preventing overfat or overthin is prepared from nucleic acid compounds, hormone compound, carbon-group compound and vitamine compounds, and features that the excessive fat and energy substances can be transferred to the important structural matters of body.