Efficient extraction method of selenium-enriched mushroom powder selenium polysaccharide

A technology of selenium-enriched mushroom powder and extraction method, which is applied in the field of polysaccharide extraction, can solve the problems of unsatisfactory absorption and utilization, ineffectiveness, and low intake, and achieve the best application value and market potential with low usage , The effect of increasing the dissolution rate

Inactive Publication Date: 2020-10-09
湖南万臻生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, supplementing selenium with inorganic selenium salts is not ideal in terms of absorption and utilization, and the intake is small, which does not work, and a slight excess will have toxic side effects

Method used

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  • Efficient extraction method of selenium-enriched mushroom powder selenium polysaccharide
  • Efficient extraction method of selenium-enriched mushroom powder selenium polysaccharide
  • Efficient extraction method of selenium-enriched mushroom powder selenium polysaccharide

Examples

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Effect test

Embodiment 1

[0039] A high-efficiency extraction method for selenium-enriched oyster mushroom powder selenium polysaccharide, comprising degreasing of the mushroom powder, extraction of selenium polysaccharide with ultrasonic synergy with cellulase, alcohol precipitation, protein removal, dialysis, etc. The specific steps are:

[0040] 1) Weigh 15 g of selenium-enriched oyster mushroom powder, add 80% ethanol according to the ratio of material to liquid 1:8, reflux with stirring at 60°C for 4 hours, filter, and vacuum dry the solid at 45°C to obtain degreasing bacteria powder;

[0041]2) Weigh 10g of defatted bacteria powder, add distilled water according to the ratio of material to liquid 1:30, adjust the pH to 5, add 1mg of cellulase, ultrasonicate at 120W, 50°C for 1h, take it out and place it at 100°C for 10min, then Stir and soak at 85°C for 2 hours, centrifuge at 5000r / min for 15min to obtain the supernatant, repeat the operation twice, combine the supernatant, and condense the supern...

Embodiment 2

[0047] A high-efficiency extraction method of selenium-enriched shiitake mushroom powder selenium polysaccharides, comprising bacterial powder degreasing, ultrasonic extraction of selenium polysaccharides with cellulase, alcohol analysis, protein removal, dialysis, etc., the specific steps are:

[0048] 1) Weigh 20 g of selenium-enriched shiitake mushroom powder, add 85% ethanol according to the ratio of material to liquid 1:10, reflux and stir at 65°C for 3 hours, filter, and dry the solid in vacuum to obtain defatted bacteria powder;

[0049] 2) Weigh 12g of defatted bacteria powder, add distilled water according to the ratio of solid to liquid 1:32, adjust the pH to 5, add 2mg of cellulase, ultrasonicate at 110W, 50°C for 1h, take it out and place it at 100°C for 12min, 90°C Stir and soak at ℃ for 2 hours, centrifuge at 5000r / min for 15 minutes to obtain a supernatant, which is concentrated into a viscous liquid by rotary evaporation, which is the selenium-enriched shiitake ...

Embodiment 3

[0055] A high-efficiency extraction method of selenium polysaccharides from selenium-enriched Flammulina velutipes fungus powder, comprising degreasing the bacteria powder, extracting selenium polysaccharides with ultrasound and cellulase, alcohol analysis, protein removal, dialysis, etc., the specific steps are:

[0056] 1) Weigh 16g of selenium-enriched Flammulina velutipes bacteria powder, add 70% ethanol according to the ratio of material to liquid 1:12, reflux and stir at 65°C for 3 hours, filter, and dry the solid in vacuum to obtain defatted bacteria powder;

[0057] 2) Weigh 10g of defatted bacteria powder, add distilled water according to the ratio of material to liquid 1:40, adjust the pH to 5, add 1.2mg of cellulase, ultrasonicate at 150W, 50°C for 0.5h, take it out and place it at 98°C for 15min , stirred and soaked at 85°C for 2 hours, centrifuged at 5500r / min for 15 minutes to obtain a supernatant, which was concentrated into a viscous liquid by rotary evaporation...

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Abstract

The invention discloses an efficient extraction method of selenium-enriched mushroom powder selenium polysaccharide, which comprises the following steps: mixing selenium-rich mushroom powder with ethanol, refluxing, carrying out suction filtration, and carrying out vacuum drying to obtain degreased mushroom powder; mixing the dried degreased mushroom powder with distilled water, adjusting the pH value, adding cellulase, carrying out ultrasonic enzymolysis, heating for enzyme deactivation, carrying out high-temperature digestion, and centrifuging to obtain an extract liquid; carrying out rotaryevaporation concentration, alcohol precipitation and centrifugation on the extract liquid to obtain precipitate, and carrying out vacuum drying to obtain crude selenium polysaccharide; and redissolving the crude selenium polysaccharide with distilled water, adding neutral protease for enzymolysis, centrifuging, removing protein from the supernatant with a Sevage reagent, centrifuging, dialyzing,and freeze-drying to obtain the mushroom powder selenium polysaccharide. According to the method, the selenium polysaccharide is extracted by adopting an ultrasonic synergistic cellulase method assisted by high-temperature digestion, so that the extraction rate of the selenium polysaccharide is greatly improved; protease-Sevage reagent combined protein removal is adopted, the protein removal effect is good, and selenium polysaccharide loss is small. The extraction method is simple and feasible, the extraction rate of the selenium polysaccharide is high, impurities are few, and the method has good application value and market potential.

Description

technical field [0001] The invention relates to the technical field of polysaccharide extraction, in particular to an efficient extraction method of selenium-enriched mushroom powder selenium polysaccharide. [0002] technical background [0003] Selenium is an essential micronutrient element for animals and plants. It can form the active center of several oxides. It can promote the decomposition of peroxides in the body and protect the structure and function of cell membranes. Immunity, antagonism of heavy metal toxicity and other biological functions. Selenium affects important physiological functions of the human body by regulating the expression of at least 30 selenoproteins, some of which have been well characterized chemically and biologically, such as glutathione peroxidase (GPXs), thioredox Protein reductases (TRs). Many diseases of the human body are related to selenium deficiency, such as Keshan disease, cardiovascular and cerebrovascular diseases, cancer, diabete...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 钟世安张云山张卓敏邓丙之孟宪存
Owner 湖南万臻生物科技有限公司
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