Application of carboxylated nitrogen-containing multi-walled carbon nanotubes to weight gain and immunity enhancement of mouse

A technology of multi-walled carbon nanotubes and immune enhancement, applied in the field of biomedicine, can solve problems that have not been seen in research so far.

Active Publication Date: 2019-02-26
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the metabolism and immunity of organisms has not been seen so far.
In particular, studies on the role in weight gain and immune enhancement in mice have not been reported

Method used

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  • Application of carboxylated nitrogen-containing multi-walled carbon nanotubes to weight gain and immunity enhancement of mouse
  • Application of carboxylated nitrogen-containing multi-walled carbon nanotubes to weight gain and immunity enhancement of mouse
  • Application of carboxylated nitrogen-containing multi-walled carbon nanotubes to weight gain and immunity enhancement of mouse

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: MWCNTs N - Effect of COOH on body weight and immune level of mice

[0029] 1. Test method:

[0030] Prepare 180 Kunming white male mice free of specific pathogenic microorganisms (SPF grade), weighing 20 ± 1 g, and randomly divide them into six groups (including: five test groups and a control group), 30 in each group.

[0031] MWCNT N -COOH was prepared into different dosage concentrations with sterile physiological saline, and injected into the mice of the above six groups by way of intramuscular injection of the legs, among which the five test groups were injected with 0.3mg / Kg, 0.6mg / Kg, and 1.0mg respectively / Kg, 3.0mg / Kg and 6.0mg / Kg dosage concentrations of MWCNT N -COOH; the control group was injected with MWCNT at a dose concentration of 0.0mg / Kg N -COOH.

[0032] On the 2nd day, 7th day and 14th day after the injection, the body weight of the mice, and the liver and spleen weights of the mice were collected and collected. The blood of the mic...

Embodiment 2

[0037] Example 2: MWCNTs N -The impact of COOH on the protective effect of vaccines

[0038] 1. Test method:

[0039] Prepare 60 SPF-grade Kunming white male mice, weighing 20±2g, and randomly divide them into control group and test group, 30 in each group.

[0040] Chicken embryos were used to amplify H9N2 avian influenza virus (H9N2AIV), and the hemagglutination inhibition test detected that the virulence HA of the virus was 10 8 .

[0041] Formaldehyde was added to the toxicity of HA at a volume ratio of 0.1% to 10 8 In the virus liquid in the medium, the virus was inactivated in a shaker at 37°C for 24 hours.

[0042] The dose (1.0mg / Kg) of MWCNT N -COOH was used as an immune adjuvant in combination with formaldehyde-inactivated H9N2AIV virus to prepare H9N2AIV nano-vaccine.

[0043] The above-prepared H9N2AIV nano-vaccine was injected into the above-mentioned test group of mice through leg muscle injection. Physiological saline and formaldehyde-inactivated H9N2AIV ...

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Abstract

The invention relates to novel application of carbon nanotubes, in particular to application of carboxylated nitrogen-containing multi-walled carbon nanotubes to weight gain and immunity enhancement of a mouse. Studies in the invention find that MWCNTN-COOH with the using dose of 1.0mg / Kg can significantly increase the weight of the mouse, has a significant effect of enhancing the immune level ofthe mouse and can be used as a candidate material for a vaccine adjuvant, so that a good material basis is provided for development and utilization of a vaccine and gaps of the MWCNTN-COOH in biological metabolism and immunological research are filled.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a new application of carbon nanotubes, in particular to the application of a carboxylated nitrogen-containing multi-walled carbon nanotube in weight gain and immune enhancement of mice. Background technique [0002] Carbon nanotubes (CNTs), as a new nano-scale carbon material, have been widely used in electronic components, composite materials and energy utilization due to their unique electrical, magnetic, thermal and mechanical properties. In recent years, the exploration aimed at the application of biological properties of CNTs has become a new research field of this nanomaterial. Single wall carbon nanotubes (SWCNTs) are tubular structures (1-2 nm in diameter) composed of a single layer of graphite; while multi-wall carbon nanotubes (Multi wall carbon nanotubes, MWCNTs) are composed of multiple graphite cylinders Concentric circular structures (diameter 2-25nm) composed of body. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/44A61K47/54A61K39/39A61P37/04A61P3/00
CPCA61K33/44A61K39/39A61K2039/55505A61K2039/575A61K47/542A61P3/00A61P37/04
Inventor 唐熠刁有祥于观留
Owner SHANDONG AGRICULTURAL UNIVERSITY
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