Isolated culture method for peony ovules
A technology of in vitro culture and ovules, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of poor orientation of variety selection, low breeding effectiveness, early embryo abortion, etc., to avoid regeneration of peony and the effect of transplanting difficulty
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Embodiment 1
[0024] A kind of in vitro culture method of peony ovule, its concrete steps are:
[0025] Step 1. Take the peony ovary 15 days after fertilization and the peony ovary 49 days respectively, use mercury chloride to disinfect the peony ovary, take out the ovules, and divide the ovules into two groups, namely the 15-day ovule group and the 49-day ovary group. Day ovule group, spare;
[0026] Step 2. Take the 15-day-old ovule group in step 1, inoculate it into MS medium I with a pH of 5.4, culture at 15° C., and culture in the dark for 15 days, and set aside;
[0027] Step 3: Take the 15-day ovule group after dark culture in step 2, inoculate it into MS medium II with a pH of 5.4, culture at a temperature of 15° C., and culture it in the dark for 10 days, then the 15-day ovule group will be The MS medium II was placed in a light intensity of 1600 lux, 16 hours of light per day, cultured for 27 days, and set aside;
[0028] Step 4: Take the 15-day ovule group after 27 days of expo...
Embodiment 2
[0033] A kind of in vitro culture method of peony ovule, its concrete steps are:
[0034] Step 1. Take the peony ovary 27 days after fertilization and the peony ovary 70 days respectively, use mercury chloride to disinfect the peony ovary, take out the ovules, and divide the ovules into two groups, namely the 27-day ovule group and the 70-day ovary group. Day ovule group, spare;
[0035] Step 2. Take the 27-day ovule group in step 1, inoculate it into MS medium I with a pH of 5.7, culture at 20° C., and culture in the dark for 8 days, and set aside;
[0036] Step 3: Take the 27-day ovule group after dark culture in step 2, inoculate it into MS medium II with a pH of 5.7, culture at 20° C., and culture it in the dark for 10 days, then inoculate the 27-day ovule group The MS medium II was placed in a light intensity of 1700 lux, 16 hours of light per day, cultured for 15 days, and set aside;
[0037] Step 4: Take the 27-day ovule group after 15 days of exposure to light in ste...
Embodiment 3
[0042] A kind of in vitro culture method of peony ovule, its concrete steps are:
[0043] Step 1. Take the peony ovary 48 days after fertilization and the 90-day peony ovary respectively, use mercury chloride to disinfect the peony ovary, take out the ovules, and divide the ovules into two groups, namely the 48-day ovule group and the 90-day ovary group. Day ovule group, spare;
[0044] Step 2. Take the 48-day ovule group in step 1, inoculate it into MS medium I with a pH of 6.0, culture at 25° C., and culture in the dark for 3 days, and set aside;
[0045]Step 3: Take the 48-day ovule group after dark culture in step 2, inoculate it into MS medium II with a pH of 6.0, culture at 25°C, and culture it in the dark for 10 days, then the ovule group containing 48 days The MS medium II was placed in a light intensity of 1800 lux, 16 hours of light per day, cultured for 7 days, and set aside;
[0046] Step 4: Take the 48-day ovule group after 7 days of exposure to light in step 3,...
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