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Isolated culture method for peony ovules

A technology of in vitro culture and ovules, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of poor orientation of variety selection, low breeding effectiveness, long cycle, etc., to avoid regeneration and transplantation of peony The effect of planting difficulties

Inactive Publication Date: 2013-10-09
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional breeding method of new peony varieties has low reproduction rate, long cycle, poor orientation of variety selection, low breeding effectiveness, and early abortion of embryos in many ornamental peony varieties; while peony obtained from vegetative organs through tissue culture There are also technical bottlenecks in both intact plants or transplants, which has become an obstacle to the adoption of biotechnology for peony breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of in vitro culture method of peony ovule, its concrete steps are:

[0025] Step 1. Take the peony ovary 15 days after fertilization and the peony ovary 49 days respectively, use mercury chloride to disinfect the peony ovary, take out the ovules, and divide the ovules into two groups, namely the 15-day ovule group and the 49-day ovary group. Day ovule group, spare;

[0026] Step 2. Take the 15-day-old ovule group in step 1, inoculate it into MS medium I with a pH of 5.4, culture at 15° C., and culture in the dark for 15 days, and set aside;

[0027] Step 3: Take the 15-day ovule group after dark culture in step 2, inoculate it into MS medium II with a pH of 5.4, culture at a temperature of 15° C., and culture it in the dark for 10 days, then the 15-day ovule group will be The MS medium II was placed in a light intensity of 1600 lux, 16 hours of light per day, cultured for 27 days, and set aside;

[0028] Step 4: Take the 15-day ovule group after 27 days of expo...

Embodiment 2

[0033] A kind of in vitro culture method of peony ovule, its concrete steps are:

[0034] Step 1. Take the peony ovary 27 days after fertilization and the peony ovary 70 days respectively, use mercury chloride to disinfect the peony ovary, take out the ovules, and divide the ovules into two groups, namely the 27-day ovule group and the 70-day ovary group. Day ovule group, spare;

[0035] Step 2. Take the 27-day ovule group in step 1, inoculate it into MS medium I with a pH of 5.7, culture at 20° C., and culture in the dark for 8 days, and set aside;

[0036] Step 3: Take the 27-day ovule group after dark culture in step 2, inoculate it into MS medium II with a pH of 5.7, culture at 20° C., and culture it in the dark for 10 days, then inoculate the 27-day ovule group The MS medium II was placed in a light intensity of 1700 lux, 16 hours of light per day, cultured for 15 days, and set aside;

[0037] Step 4: Take the 27-day ovule group after 15 days of exposure to light in ste...

Embodiment 3

[0042] A kind of in vitro culture method of peony ovule, its concrete steps are:

[0043] Step 1. Take the peony ovary 48 days after fertilization and the 90-day peony ovary respectively, use mercury chloride to disinfect the peony ovary, take out the ovules, and divide the ovules into two groups, namely the 48-day ovule group and the 90-day ovary group. Day ovule group, spare;

[0044] Step 2. Take the 48-day ovule group in step 1, inoculate it into MS medium I with a pH of 6.0, culture at 25° C., and culture in the dark for 3 days, and set aside;

[0045]Step 3: Take the 48-day ovule group after dark culture in step 2, inoculate it into MS medium II with a pH of 6.0, culture at 25°C, and culture it in the dark for 10 days, then the ovule group containing 48 days The MS medium II was placed in a light intensity of 1800 lux, 16 hours of light per day, cultured for 7 days, and set aside;

[0046] Step 4: Take the 48-day ovule group after 7 days of exposure to light in step 3,...

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Abstract

The invention relates to an isolated culture method for peony ovules. After peony ovaries which are fertilized for 15 to 90 days are disinfected by mercuric chloride, the ovules are taken out and divided into a 15-to-48-day ovule group and a 49-to-90-day ovule group; the 15-to-48-day ovule group is inoculated into MS culture medium, cultured in the dark for 3 to 15 days, then inoculated into MS culture medium II, cultured in the dark for 10 days and then cultured in light for 7 to 27 days; afterwards, the 15-to-48-day ovule group is inoculated into the MS culture medium II again and cultured in light for 8 to 28 days, so that mature peony seeds I are obtained; and after being first cultured in the dark for 10 days, the 49-to-90-day ovule group is cultured in light for 7 to 27 days, then inoculated in the MS culture medium II again and cultured in light for 8 to 28 days, so that mature peony seeds II are obtained. The isolated culture method for peony ovules can be used in the isolated research on peony embryo development, and solves the problems of the early abortion of the embryos of good-looking peony varieties and the abortion of distant somatic hybrid peonies, and by way of ovule culture, the improved research on peony varieties can be carried out as well.

Description

technical field [0001] The invention relates to a method for cultivating peony ovules, in particular to a method for in vitro culturing of peony ovules. Background technique [0002] Peony is a perennial deciduous subshrub belonging to the family Paeoniaceae. It is a traditional famous flower in China. cherish. And peony not only has ornamental value, but also has high medicinal value. [0003] As the basis for the development of the entire industry, peony varieties need to be continuously introduced to cultivate new varieties with different flower colors, flower shapes, flower fragrances, and flowering periods. The traditional breeding method of new peony varieties has low reproduction rate, long cycle, poor orientation of variety selection, low breeding effectiveness, and early abortion of embryos in many ornamental peony varieties; while peony obtained from vegetative organs through tissue culture There are also technical bottlenecks in whole plants or transplanting, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张改娜冯书营史国安
Owner HENAN UNIV OF SCI & TECH
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