Method for establishing catalpa bungei embryonal cell suspension system

An embryogenic cell and establishment method technology, which is applied in the field of establishment of catalpa tree embryogenic cell suspension line, can solve the problems of few, unseen, difficulty in reproduction and rooting, etc., and achieves the promotion of cell proliferation, avoidance of browning and death, and induction of differentiation. powerful effect

Active Publication Date: 2017-04-19
CHINA THREE GORGES UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Because catalpa is self-fertile, it often blooms but does not bear fruit, the germination rate of seeds is very low, and it is difficult to propagate and take root by cutting method, which causes great obstacles to the propagation and popularization of excellent strains of catalpa. The development and utilization of catalpa, resulting in the lack of catalpa resources
[0004] At present, there are not many studies on the somatic embryogenesis of Catalpa, which mainly focus on the study of cell morphology, physiology and biochemistry, and the initial research on the induction of somatic embryogenesis in the process of somatic embryogenesis. A Report on Suspension Culture of Somatic Embryos

Method used

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  • Method for establishing catalpa bungei embryonal cell suspension system
  • Method for establishing catalpa bungei embryonal cell suspension system
  • Method for establishing catalpa bungei embryonal cell suspension system

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Embodiment 1

[0030] MS Basic Medium: It can be prepared by referring to the literature (Murashige T, Skoog F. A revised medium for rapidgrouth and bioassays with tobacco tissue cultures. Physiol. Plant, 1962, 15:473-497).

[0031] A method for establishing a Chinese catalpa embryogenic cell suspension line, comprising the following steps:

[0032] 1) Sterilization of explants and acquisition of embryogenic callus of Catalpa chinensis:

[0033] For explant sterilization, take the immature seeds of Catalpa chinensis, after being sterilized by 75% alcohol and 2% sodium hypochlorite solution successively, strip off the young seed embryos as explants; place the explants in a Induced in a series of solid medium, pale yellow embryogenic callus was obtained.

[0034] The step 1) method for obtaining embryogenic callus of Chinese catalpa comprises: ① induction of callus: using sterilized young seed embryos of Chinese catalpa as explants, placing it on the callus induction medium Induce callus; in...

Embodiment 2

[0041] MS Basic Medium: It can be prepared by referring to the literature (Murashige T, Skoog F. A revised medium for rapidgrouth and bioassays with tobacco tissue cultures. Physiol. Plant, 1962, 15:473-497).

[0042] The method that catalpa suspension culture system of the present invention is established comprises the following steps:

[0043] 1) Sterilization of explants and acquisition of embryogenic callus

[0044] For explant sterilization, take the immature seeds of Catalpa chinensis, after being sterilized by 75% alcohol and 2% sodium hypochlorite solution successively, strip off the young seed embryos as explants; place the explants in a Induced in a series of solid medium, pale yellow embryogenic callus was obtained.

[0045] The step 1) method for obtaining embryogenic callus of Chinese catalpa comprises: ① induction of callus: using sterilized young seed embryos of Chinese catalpa as explants, placing it on the callus induction medium Induce callus; induction med...

Embodiment 3

[0054] MS Basic Medium: It can be prepared by referring to the literature (Murashige T, Skoog F. A revised medium for rapidgrouth and bioassays with tobacco tissue cultures. Physiol. Plant, 1962, 15:473-497).

[0055] The method that catalpa suspension culture system of the present invention is established comprises the following steps:

[0056] 1) Sterilization of explants and acquisition of embryogenic callus

[0057] For explant sterilization, take the immature seeds of Catalpa chinensis, after being sterilized by 75% alcohol and 2% sodium hypochlorite solution successively, strip off the young seed embryos as explants; place the explants in a Induced in a series of solid medium, pale yellow embryogenic callus was obtained.

[0058] The step 1) method for obtaining embryogenic callus of Chinese catalpa comprises: ① induction of callus: using sterilized young seed embryos of Chinese catalpa as explants, placing it on the callus induction medium Induce callus; induction med...

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Abstract

The invention belongs to the technical field of cell culture, and relates to a catalpa bungei embryonal cell suspension culture method, wherein the method comprises a step of taking embryos of immature fruits of catalpa bungei as explants, and sequentially comprises the steps of inducing embryonal callus, inducing an initial suspension culture product, establishing an embryonal cell suspension system, and regenerating plants; the induction culture medium of the initial suspension culture product is 1 / 2MS; and, the culture solution of the catalpa bungei embryonal cell suspension system is that polyethylene glycol (PEG) 6000 is added based on the 1 / 2MS culture medium. By means of the method disclosed by the invention, the steady catalpa bungei embryonal cell suspension system can be established; furthermore, the plantlet survival rate of embryo regeneration plants is high; emerged seedlings are tidy and normal; and the method disclosed by the invention can provide a steady material source and a platform for catalpa bungei embryo development, somatic hybridization, genetic transformation, and storage and research of plant germplasm resources, and provide important theoretical and experimental basis for industrial breeding of a bioreactor technology based on suspension cell culture.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and relates to a method for establishing a suspension line of embryogenic cells of Chinese catalpa. Background technique [0002] Catalpa bungei C.A. Meyer. belongs to Bignoniaceae (Bignoniaceae) catalpa (Catalpa), native to China, is a unique precious tree species in my country, known as the "King of Wood". There are about 13 species of plants in the genus Asteraceae in the world, mainly distributed in America and East Asia (Guo Congjian et al., 1994). In my country, catalpa is distributed from the warm temperate zone to the subtropical zone, and is mostly distributed in Shandong, Jiangsu, Henan, and Anhui provinces (Shi Xin et al., 2011). [0003] Catalpa has a long history of utilization, and it is a precious timber tree species and a famous garden ornamental tree species (Tang Ling et al., 2007). Catalpa is listed as an important timber species by the country. Its wood has the characte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 梁宏伟陈发菊高晗王长兰王玉兵张德春沈祥陵刘文
Owner CHINA THREE GORGES UNIV
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