Decaisnea insignis isolated culture method

Abstract

The invention discloses a Decaisnea insignis isolated culture method. The method comprises that an immature fruit seed embryo as an explant is orderly subjected to callus induction, adventitious bud differentiation induction, adventitious bud enrichment culture and rooting induced culture so that a Decaisnea insignis regeneration plant is obtained. Before callus induction, the method also comprises explant disinfection: taking tender Decaisnea insignis fruits, immersing the tender Decaisnea insignis fruits in a cleaning solution for 20-30min, wherein each 100ml of the cleaning solution contains 6-10 droplets of a cleaning agent, then scrubbing the surfaces of the Decaisnea insignis fruits, washing the Decaisnea insignis fruits through water for 1-2h, carrying out disinfection treatment on the tender Decaisnea insignis fruits through alcohol with a concentration of 75% in an aseptic environment for 30-60s, carrying out disinfection on the Decaisnea insignis fruits through a HgCl2 solution with a concentration of 0.1% for 15min, wherein each 100ml of the HgCl2 solution contains 3-4 droplets of Tween-40, cleaning the Decaisnea insignis fruits through sterile water 4-6 times, for 1-3min every time, taking out tender seeds, extruding tender seed embryos and rinsing the tender seed embryos through sterile water to obtain explants. The method realizes high frequency induction of a lot of Decaisnea insignis clustered adventitious buds and has a high transplanting survival rate. The method lays a good foundation of Decaisnea insignis propagation coefficient improvement and specie germplasm preservation, promotion and use.

Description

technical field [0001] The invention relates to a method for in vitro cultivation of cat feces. Background technique [0002] Decaisnea insignis (Griff.) Hook.f.et Thoms.) is an upright deciduous shrub of the genus Decaisnea in the family Lardizabalaceae. It is widely distributed in the southwestern to central regions of my country, growing at an altitude of 900 -3600m hillside shrubs or damp places under miscellaneous forests in valleys (Ying Junsheng et al., 2001). Maoershi, also known as catshit melon, comes from the fact that its fruit is blue-purple long strips, slightly curved, resembling catshit (Hou Yunhe et al., 2011). [0003] The fruit of cat feces is a berry, which is rich in juice, rich in sugar, vitamins and mineral nutrients, etc. It can be eaten as fruit, and can also be used to produce fruit juice, wine and sugar; the peel contains a lot of rubber components, and its proportion It can reach 10%-20%, and can be used for the production of rubber products (Hou...

AI Technical Summary

Problems solved by technology

In recent years, for the research on tissue culture of cat feces, there are only sterile seedlings and axillary buds used as explants to induce adventitious buds (Yang Jingtian et al. 2009; Guo Bangli 2013). Effects of combination and ratio on induction of axillary and adventitious buds, but with low reproductive coefficients

Images