In vitro culture method of cat feces

A technology of in vitro culture and cat feces, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problem of low reproduction coefficient, achieve an increase in reproduction coefficient, broad application prospects, and strong ability to induce differentiation Effect

Inactive Publication Date: 2019-03-12
CHINA THREE GORGES UNIV +1
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, for the research on tissue culture of cat feces, there are only sterile seedlings and axillary buds used as explants to induce adventitious buds (Yang Jingtian et al. 2009; Guo Bangli 2013). Effects of combination and ratio on induction of axillary and adventitious buds, but with low reproductive coefficients

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • In vitro culture method of cat feces
  • In vitro culture method of cat feces
  • In vitro culture method of cat feces

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1, the in vitro culture of cat feces

[0053] MS basal medium: It can be prepared by referring to the literature (Murashige T, Skoog F.A revised medium for rapid groove and bioassays with tobacco tissue cultures. Physiol. Plant, 1962, 15:473-497).

[0054] The in vitro culture method of cat feces of the present invention comprises the following steps:

[0055] (1) Sterilization of young fruit explants

[0056] Take the young and tender fruits of cat feces, soak them for 30 minutes in the cleaning liquid with a concentration of 6-10 drops per 100ml, then scrub the surface, then rinse them with tap water for 2 hours, put them in an ultra-clean workbench, and use 75% Soak in alcohol for 60s, then use 0.1% HgCl containing 4 drops of soil temperature 40 per 100ml 2 The solution was sterilized for 15 minutes and washed 5 times with sterile water. Wear sterile gloves to peel off the young seeds in the fruit, squeeze out the young seed embryos, rinse with sterile ...

Embodiment 2

[0072] Embodiment 2, the in vitro culture of cat feces

[0073]MS basal medium: It can be prepared by referring to literature (Murashige T, Skoog F. Arevised medium for rapidgrouth and bioassays with tobacco tissue cultures. Physiol. Plant, 1962, 15:473-497).

[0074] The in vitro culture method of cat feces of the present invention comprises the following steps:

[0075] (1) Sterilization of young fruit explants

[0076] Take the young and tender fruits of cat feces, soak them in the cleaning solution containing 4-8 drops per 100ml for 20 minutes, then scrub the surface, then rinse them with tap water for 1 hour, and put them in the ultra-clean workbench. Soak in alcohol for 40s, then use 0.1% HgCl containing 3 drops of soil temperature 40 per 100ml 2 The solution was sterilized for 15 minutes and washed 4 times with sterile water. Wear sterile gloves to peel off the young seeds in the fruit, squeeze out the young seed embryos, rinse with sterile water and inoculate them a...

Embodiment 3

[0092] Embodiment 3, the in vitro culture of cat feces

[0093] MS basal medium: It can be prepared by referring to literature (Murashige T, Skoog F. Arevised medium for rapidgrouth and bioassays with tobacco tissue cultures. Physiol. Plant, 1962, 15:473-497).

[0094] The in vitro culture method of cat feces of the present invention comprises the following steps:

[0095] (1) Sterilization of young fruit explants

[0096] Take the young and tender fruits of cat feces, soak them for 30 minutes in the cleaning liquid with a concentration of 6-10 drops per 100ml, then scrub the surface, then rinse them with tap water for 2 hours, put them in an ultra-clean workbench, and use 75% Soak in alcohol for 60s, then use 0.1% HgCl containing 5 drops of soil temperature 40 per 100ml 2 The solution was sterilized for 15 minutes and washed 5 times with sterile water. Wear sterile gloves to peel off the young seeds in the fruit, squeeze out the young seed embryos, rinse with sterile water...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a Decaisnea insignis isolated culture method. The method comprises that an immature fruit seed embryo as an explant is orderly subjected to callus induction, adventitious bud differentiation induction, adventitious bud enrichment culture and rooting induced culture so that a Decaisnea insignis regeneration plant is obtained. Before callus induction, the method also comprises explant disinfection: taking tender Decaisnea insignis fruits, immersing the tender Decaisnea insignis fruits in a cleaning solution for 20-30min, wherein each 100ml of the cleaning solution contains 6-10 droplets of a cleaning agent, then scrubbing the surfaces of the Decaisnea insignis fruits, washing the Decaisnea insignis fruits through water for 1-2h, carrying out disinfection treatment on the tender Decaisnea insignis fruits through alcohol with a concentration of 75% in an aseptic environment for 30-60s, carrying out disinfection on the Decaisnea insignis fruits through a HgCl2 solution with a concentration of 0.1% for 15min, wherein each 100ml of the HgCl2 solution contains 3-4 droplets of Tween-40, cleaning the Decaisnea insignis fruits through sterile water 4-6 times, for 1-3min every time, taking out tender seeds, extruding tender seed embryos and rinsing the tender seed embryos through sterile water to obtain explants. The method realizes high frequency induction of a lot of Decaisnea insignis clustered adventitious buds and has a high transplanting survival rate. The method lays a good foundation of Decaisnea insignis propagation coefficient improvement and specie germplasm preservation, promotion and use.

Description

technical field [0001] The invention relates to a method for in vitro cultivation of cat feces. Background technique [0002] Decaisnea insignis (Griff.) Hook.f.et Thoms.) is an upright deciduous shrub of the genus Decaisnea in the family Lardizabalaceae. It is widely distributed in the southwestern to central regions of my country, growing at an altitude of 900 -3600m hillside shrubs or damp places under miscellaneous forests in valleys (Ying Junsheng et al., 2001). Maoershi, also known as catshit melon, comes from the fact that its fruit is blue-purple long strips, slightly curved, resembling catshit (Hou Yunhe et al., 2011). [0003] The fruit of cat feces is a berry, which is rich in juice, rich in sugar, vitamins and mineral nutrients, etc. It can be eaten as fruit, and can also be used to produce fruit juice, wine and sugar; the peel contains a lot of rubber components, and its proportion It can reach 10%-20%, and can be used for the production of rubber products (Hou...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 梁宏伟陈发菊魏文桃陈芸芸杨敬元杨林森
Owner CHINA THREE GORGES UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap